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| Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis |
| LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa |
1 Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R.China
2 College of Life Sciences, Beijing Normal University, Beijing 100875, P.R.China
3 COE For Neuroscience, Departments of Anesthesiology, Biomedical Sciences, Texas Tech University Health Sciences Center, Texas
79905, USA |
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摘要 Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5´-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.
Abstract Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5´-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.
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Received: 10 November 2012
Accepted:
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| Fund: This work was kindly supported by the National Natural Sciences Foundation of China (30970286, 31270362 and 31070289). |
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Corresponding Authors:
Correspondence ZHANG Gen-fa, Tel: +86-10-58809453, Fax: +86-10-58807720, E-mail: gfzh@bnu.edu.cn
E-mail: gfzh@bnu.edu.cn
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| About author: LIU Xiao-fei, E-mail: lxf541@163.com |
Cite this article:
LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa.
2013.
Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis. Journal of Integrative Agriculture, 12(10): 1722-1730.
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| [1]Apel K, Hirt H. 2004. Reactive oxygen species: metabolism,oxidative stress, and signal transduction. Annual Reviewof Plant Biology, 55, 373-399[2]Clough S J, Bent A F. 1998. Floral dip: a simplifiedmethod for Agrobacterium-mediated transformation ofArabidopsis thaliana. The Plant Journal, 16, 735-743[3]le Dreau Y, Dupuy N, Gaydou V, Joachim J, Kister J. 2009.Study of jojoba oil aging by FTIR. Aalytica ChimicaActa, 642, 163-170[4]Dunn M A, White A J, Vural S, Hughes M A. 1998.Identification of promoter elements in a low-temperatureresponsivegene (blt4.9) from barley (Hordeum vulgareL.). Plant Molecular Biology, 38, 551-564[5]El-Mallah M H, El-Shami S M. 2009. Investigation of liquidwax components of Egyptian jojoba seeds. Journal ofOleo Science, 58, 543-548[6]Foyer C H, Noctor G. 2005. Redox homeostasis andantioxidant signaling: a metabolic interface betweenstress perception and physiological responses. The PlantCell, 17, 1866-1875[7]Fridovich I. 1995. Superoxide radical and superoxidedismutases. Annual Review of Biochemistry, 64, 97-112[8]Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M,Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K,Yamaguchi-Shinozaki K. 2005. AREB1 is a transcriptionactivator of novel ABRE-dependent ABA signaling thatenhances drought stress tolerance in Arabidopsis. The Plant Cell, 17, 3470-3488[9]Geng H W, Shi L, Li W, Zhang B, Chu C C, Li H J, Zhang G F. 2008. Gene expression of jojoba (Simmondsia chinensis) leaves exposed to drying. Environmental and Experimental Botanyt, 63, 137-146[10]Han Y J, Cho K C, Hwang O J, Choi Y S, Shin A Y, Hwang I, Kim J I. 2012. Overexpression of an Arabidopsis beta-glucosidase gene enhances drought resistance with dwarf phenotype in creeping bentgrass. Plant Cell Reports, 31, 1677-1686[11]Hu T, He S, Yang G, Zeng H, Wang G, Chen Z, Huang X. 2011. Isolation and characterization of a rice glutathione S-transferase gene promoter regulated by herbicides and hormones. Plant Cell Reports, 30, 539-549[12]Jefferson R A, Kavanagh T A, Bevan M W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal, 6, 3901-3907[13]Kim J S, Mizoi J, Yoshida T, Fujita Y, Nakajima J, Ohori T, Todaka D, Nakashima K, Hirayama T, Shinozaki K, et al. 2011. An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiology, 52, 2136-2146[14]Mbah C J. 2007. Studies on the lipophilicity of vehicles (or co-vehicles) and botanical oils used in cosmetic products. Pharmazie, 62, 351-353[15]McKersie B D, Murnaghan J, Jones K S, Bowley S R. 2000. Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiology, 122, 1427-1437[16]Miller G, Suzuki N, Rizhsky L, Hegie A, Koussevitzky S, Mittler R. 2007. Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses. Plant Physiology, 144, 1777-1785[17]Mittler R, Vanderauwera S, Gollery M, van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends in Plant Science, 9, 490-498[18]Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410[19]Polle A. 2001. Dissecting the superoxide dismutase-ascorbate-glutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiology, 126, 445-462[20]Prashanth S R, Sadhasivam V, Parida A. 2008. Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance Transgenic Research, 17, 281-291[21]Rizhsky L, Liang H, Mittler R. 2003. The water-water cycle is essential for chloroplast protection in the absence of stress. Journal of Biological Chemistry, 278, 38921- 38925. [22]Takahashi M A, Asada K. 1983. Superoxide anion permeability of phospholipid membranes and chloroplast thylakoids. Archives of Biochemistry and Biophysics, 226, 558-566[23]Thangadurai C, Suthakaran P, Barfal P, Anandaraj B, Pradhan S N, Ramalingam S, Murugan V. 2008. Rare codon priority and its position specificity at the 5’ of the gene modulates heterologous protein expression in Escherichia coli. Biochemical Biophysical Resarch Communications, 376, 647-652[24]Touitou E, Godin B. 2008. Skin nonpenetrating sunscreens for cosmetic and pharmaceutical formulations. American Journal of Clinical Dermatology, 26, 375-379[25]Tran L S, Nakashima K, Sakuma Y, Simpson S D, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2004. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. The Plant Cell, 16, 2481-2498[26]Wan X R, Li L. 2006. Regulation of ABA level and water-stress tolerance of Arabidopsis by ectopic expression of a peanut 9-cis-epoxycarotenoid dioxygenase gene. Biochemical Biophysical Resarch Communications, 347, 1030-1038[27]Wang F Z, Wang Q B, Kwon S Y, Kwak S S, Su W A. 2005. Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. Journal of Plant Physiology, 162, 465-472[28]Wang Y C, Qu G Z, Li H Y, Wu Y J, Wang C, Liu G F, Yang C P. 2010. Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii. Molecular Biology Reports, 37, 1119-1124[29]Xie X B, Li S, Zhang R F, Zhao J, Chen Y C, Zhao Q, Yao Y X, You C X, Zhang X S, Hao Y J. 2012. The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples. Plant Cell and Environment, 35, 1884-1897[30]Xu Z, Zhou G, Shimizu H. 2010. Plant responses to drought and rewatering. Plant Signal Behaviour, 5, 649-654[31]Yamaguchi-Shinozaki K, Shinozaki K. 1994. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. The Plant Cell, 6, 251-264[32]Yamaguchi-Shinozaki K, Shinozaki K. 2005. Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends in Plant Science, 10, 88-94. |
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