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Journal of Integrative Agriculture  2018, Vol. 17 Issue (10): 2204-2214    DOI: 10.1016/S2095-3119(17)61897-5
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Overexpression of AmDUF1517 enhanced tolerance to salinity, drought, and cold stress in transgenic cotton
HAO Yu-qiong*, LU Guo-qing*, WANG Li-hua, WANG Chun-ling, GUO Hui-ming, LI Yi-fei, CHENG Hong-mei
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
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Abstract  As abiotic stresses become more severe as a result of global climate changes, the growth and development of plants are restricted. In the development of agricultural crops with greater stress tolerance, AmDUF1517 had been isolated from the highly stress-tolerant shrub Ammopiptanthus mongolicus, and can significantly enhance stress tolerance when inserted in Arabidopsis thaliana. In this study, we inserted this gene into cotton to analyze its potential for conferring stress tolerance. Two independent transgenic cotton lines were used. Southern blot analyses indicated that AmDUF1517 was integrated into the cotton genome. Physiological analysis demonstrated that AmDUF1517-transgenic cotton had stronger resistance than the control when treated with salt, drought, and cold stresses. Further analysis showed that trans-AmDUF1517 cotton displayed significantly higher antioxidant enzyme (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione S-transferase (GST)) activity and less reactive oxygen species (ROS) accumulation, which suggests that overexpression of AmDUF1517 can improve cotton resistance to stress by maintaining ROS homeostasis, as well as by alleviating cell membrane injury. These results imply that AmDUF1517 is a candidate gene in improving cotton resistance to abiotic stress. 
Keywords:  transgenic cotton        stress tolerance        AmDUF1517        Ammopiptanthus mongolicus        reactive oxygen species  
Received: 27 October 2017   Accepted:
Fund: This work was financially supported by the Key Project for Breeding Genetic Modified Organisms, China (2016ZX08005004) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
Corresponding Authors:  Correspondence CHENG Hong-mei, Tel/Fax: +86-10-82106125, E-mail:chenghongmei@caas.cn   
About author:  HAO Yu-qiong, E-mail: haoyuqiong334@163.com; * These authors contributed equally to this study.

Cite this article: 

HAO Yu-qiong, LU Guo-qing, WANG Li-hua, WANG Chun-ling, GUO Hui-ming, LI Yi-fei, CHENG Hong-mei. 2018. Overexpression of AmDUF1517 enhanced tolerance to salinity, drought, and cold stress in transgenic cotton. Journal of Integrative Agriculture, 17(10): 2204-2214.

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