|
|
|
|
|
| Using the Phosphomannose Isomerase (PMI) Gene from Saccharomyces cerevisiae for Selection in Rice Transformation |
| WANG Tao, LIU Liang-yu, TANG Yong-yan, ZHANG Xiao-bo, ZHANG Mei-dong, ZHENG Yong-lian, ZHANG Fang-dong |
| National Key Laboratory of Crop Genetic Improvement, Ministry of Science and Technology/Huazhong Agricultural University, Wuhan 430070,P.R.China |
|
|
|
摘要 The phosphomannose isomerase (PMI) gene from Saccharomyces cerevisiae acted as selectable marker and mannose acted as selective agent for the production of transgenic plants of rice (Oryza sativa L.) via Agrobacterium-mediated transformation. The concentration of mannose during the selection was stepwise increased, 5 g L-1 mannose combined with 15 g L-1 sucrose and 500 mg L-1 cefotaxime was used in the initial selection stage, then the concentration of mannose was increased to 11 g L-1, the highest transformation rate was 20.0%. The integration of PMI gene was confirmed by PCR, and the result of RT-PCR assay proved that the intron of PMI gene can be excised correctly during RNA splicing. β- Glucuronidase (GUS) activity analysis confirmed the expression of GUS gene. All those means the PMI gene from yeast can be used as a selectable marker in rice transformation.
Abstract The phosphomannose isomerase (PMI) gene from Saccharomyces cerevisiae acted as selectable marker and mannose acted as selective agent for the production of transgenic plants of rice (Oryza sativa L.) via Agrobacterium-mediated transformation. The concentration of mannose during the selection was stepwise increased, 5 g L-1 mannose combined with 15 g L-1 sucrose and 500 mg L-1 cefotaxime was used in the initial selection stage, then the concentration of mannose was increased to 11 g L-1, the highest transformation rate was 20.0%. The integration of PMI gene was confirmed by PCR, and the result of RT-PCR assay proved that the intron of PMI gene can be excised correctly during RNA splicing. β- Glucuronidase (GUS) activity analysis confirmed the expression of GUS gene. All those means the PMI gene from yeast can be used as a selectable marker in rice transformation.
|
|
Received: 16 May 2011
Accepted:
|
| Fund: This study was supported by the Genetically modified organisms breeding major projects，China（2011ZX08003-003） |
|
Corresponding Authors:
Correspondence ZHANG Fang-dong, Tel: +86-27-87282689, E-mail: fdzhang@mail.hzau.edu.cn
|
| About author: WANG Tao, E-mail: wt2915@163.com; |
Cite this article:
WANG Tao, LIU Liang-yu, TANG Yong-yan, ZHANG Xiao-bo, ZHANG Mei-dong, ZHENG Yong-lian, ZHANG Fang-dong.
2012.
Using the Phosphomannose Isomerase (PMI) Gene from Saccharomyces cerevisiae for Selection in Rice Transformation. Journal of Integrative Agriculture, 12(9): 1391-1398.
|
| [1]Baskin A T I, Remillong E L, Wilson J E. 2001. The impact of mannose and other carbon sources on the elongation and diameter of the primary root of Arabidopsis thaliana. Plant Physiology, 28, 481-488. [2]Boscariol R L, Almeida W A B, Derbyshire M T V C, Mourao F A A, Mendes B M J. 2003. The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck). Plant Cell Reports, 22, 122-128. [3]Briza J, Pavingerova D, Prikrylova P, Gazdova J, Vlasak J, Niedermeierova H. 2008. Use of phosphomannose isomerase-based selection system for Agrobacteriummediated transformation of tomato and potato. Biologia Plantarum, 52, 453-461. [4]Briza J, Ruzickova N, Niedermeierova H, Dusbaskova J, Vlasak J. 2010. Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation. Acta Biochimica Polonica, 57, 63-68. [5]Degenhardt J, Poppe A, Montag J, Szankowski I. 2006. The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants. Plant Cell Reports, 25, 1149-1156. [6]Dellaporta S L, Wood J, Hicks J B. 1983. A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1, 19-21. [7]Gadaleta A, Giancaspro A, Blechl A, Blanco A. 2006. Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. Journal of Cereal Science, 43, 31-37. [8]Gao Z S, Xie X J, Ling Y, Muthukrishnan S, Liang G H. 2005. Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system. Plant Biotechnology Journal, 3, 591-599. [9]Goldsworthy A, Street H E. 1965. The carbohydrate nutrition of tomato roots. VIII. The mechanism of the inhibition by D-mannose of the respiration of excised roots. Annals of Botany, 29, 45-58. [10]Haldrup A, Petersen S G, Okkels F T. 1998. Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry. Plant Cell Reports, 18, 76-81. [11]He Z Q, Duan Z Z, Liang W, Chen F J, Yao W, Liang H W, Yue C Y, Sun Z X, Chen F, Dai J W. 2006. Mannose selection system used for cucumber transformation. Plant Cell Reports, 25, 953-958. [12]He Z Q, Fu Y P, Si H M, Hu G C, Zhang S H, Yu Y H, Sun Z X. 2004. Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium. Plant Science, 166, 17-22. [13]Jain M, Chengalrayan K, Abouzid A, Gallo M. 2007. Prospecting the utility of a PMI/mannose selection system for the recovery of transgenic sugarcane (Saccharum spp. hybrid) plants. Plant Cell Reports, 26, 581-590. [14]Jang J C, Sheen J. 1994. Sugar sensing in higher plants. The Plant Cell, 6, 1665-1679. [15]Jang J C, Sheen J. 1997. Sugar sensing in higher plants. Trends in Plant Science, 2, 208-214. [16]Jeferson R A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter, 5, 387-405. [17]Joersbo M, Donaldson I, Kreiberg J, Petersen S G, Brunstedt J, Okkels F T. 1998. Analysis of mannose selection used for transformation of sugar beet. Molecular Breeding, 4, 111-117. [18]Joersbo M, Okkels F T. 1996. A novel principle for selection of transgenic plant cells: positive selection. Plant Cell Reports, 6, 219-221. [19]Lee B T, Matheson N K. 1984. Phosphomannoisomerase and phosphoglucoisomerasein seeds of Cassia coluteoides and some other legumes that synthesize galactomannan. Phytochemistry, 23, 983-987. [20]Lin Y J, Chen H, Cao Y L, Wu C Y, Wen J, Li Y F, Hua H X. 2002. Establishment of high-efficiency Agrobacteriummediated transformation system of Mudanjiang 8. Acta Agronomica Sinica, 28, 294-300. (in Chinese) [21]Lucca P, Ye X, Potrykus I. 2001. Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Molecular Breeding, 7, 43-49. [22]Miles J, Guest J. 1984. Nucleotide sequence and transcriptional start point of the phosphomannoseisomerase gene (manA) of Escherichia coli. Gene, 2, 41-48. [23]Min B W, Cho Y N, Song M J, Noh T K, Kim B K, Chae W K, Park Y S, Choi Y D, Harn C H. 2007. Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker. Plant Cell Reports, 26, 337-344. [24]Negrotto D, Jolley M, Beer S, Wenck A R, Hansen G. 2000. The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants (Zea mays L.) via Agrobacterium transformation. Plant Cell Reports, 19, 798-803. [25]Pego J V, Weisbeek P J, Smeekens S C M. 1999. Mannose inhibits Arabidopsis thaliana germination via a hexokinase-mediated step. Plant Physiology, 119, 1017-1023. [26]Rook F, Hadingham S A, Li Y, Bevan M W. 2006. Sugar and ABA response pathways and the control of gene expression. Plant Cell and Environment, 29, 426-434. [27]Sheu-Hwa C S, Lewis D H, Walker D A. 1975. Stimulation of photosynthetic starch formation by sequestration of cytoplasmic orthophosphate. New Phytologist, 74, 383-392. [28]Sigareva M, Spivey R, Willits M, Kramer C, Chang Y F. 2004. An efficient mannose selection protocol for tomato that has no adverse effect on the ploidy level of transgenic plants. Plant Cell Reports, 23, 236-245. [29]Todd R, Tague B W. 2001. Phosphomannose isomerase: a versatile selectable marker for Arabidopsis thaliana germ-line transformation. Plant Molecular Biology Reporter, 19, 307-319. [30]Vaccari I, Martinelli L. 2009. Evaluation of the phosphomannose isomerase-based selection system for gene transfer in grape. Vitis, 48, 137-144. [31]Wang A S, Evans R A, Altendorf P R, Hanten J A, Doyle M C, Rosichan J L. 2000. A mannose selection system for production of fertile transgenic maize plants from protoplasts. Plant Cell Reports, 19, 654-660. [32]Wright M, Dawson J, Dunder E, Suttie J, Reed J, Kramer C, Chang Y, Novitzky R, Wang H, Artim-Moore L. 2001. Efficient biolistic transformation of maize (Zea mays L.) and wheat (Triticum aestivum L.) using the phosphomannose isomerase gene, pmi, as the selectable marker. Plant Cell Reports, 20, 429-436. [33]Yuan K, Wysocka-Diller J. 2006. Phytohormone signalling pathways interact with sugars during seed germination and seedling development. Journal of Experimental Botany, 57, 3359-3367. [34]Zhou X L, Shen W, Rao Z M, Wang Z, Xiang Z J. 2004. A rapid method for reparation of fungal chromosome DNA. Microbiology, 31, 89-92. (in Chinese) [35]Zhu Y J, Agbayani R, McCafferty H, Albert H H, Moore P H. 2005. Effective selection of transgenic papaya plants with the PMI/Man selection system. Plant Cell Reports, 24, 426-432. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
