|
|
|
|
|
| Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff. |
| HE Wen-ai, HUANG Da-hui, LI Rong-bai, YANG Hai-ning, HUANG Yue-yue, LIU Chi, MA Zeng-feng, YANG Yong |
1.Guangxi University Library, Guangxi University, Nanning 530004, P.R.China
2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Rice Research Institute, Guangxi Academy of Agricultural Sciences,Nanning 530007, P.R.China
3.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, P.R.China
4.Agricultural College, Guangxi University, Nanning 530004, P.R.China |
|
|
|
摘要 Bacterial leaf streak (BLS) of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, bls1, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 (9311/DP3//9311) population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BC2F2 in 1:3 ratio (χ2=0.009, χ2 0.05, 1=3.84, P>0.05), suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis (BSA), two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as bls1, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6.
Abstract Bacterial leaf streak (BLS) of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, bls1, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 (9311/DP3//9311) population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BC2F2 in 1:3 ratio (χ2=0.009, χ2 0.05, 1=3.84, P>0.05), suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis (BSA), two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as bls1, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6.
|
|
Received: 22 September 2011
Accepted:
|
| Fund: This research was supported by the Science Foundation of Guangxi University, China (XDZ110082), the National Natural Science Foundation of China (31000703), the Guangxi Science and Technology Projects, China (1123001- 3B), the Guangxi Science Foundation of China (0833078) and the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences, China (200801Z and 200918J). |
|
Corresponding Authors:
LI Rong-bai, Mobile: 13517664886, E-mail: lirongbai@126.com; HUANG Da-hui, Mobile: 13878852855, E-mail: hdh1103@163.com
E-mail: lirongbai@126.com
|
| About author: HE Wen-ai, Tel/Fax: +86-771-3271163, Mobile: 13978685143, E-mail: hwamch@163.com |
Cite this article:
HE Wen-ai, HUANG Da-hui, LI Rong-bai, YANG Hai-ning, HUANG Yue-yue, LIU Chi, MA Zeng-feng, YANG Yong .
2012.
Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.. Journal of Integrative Agriculture, 12(6): 962-969.
|
| [1]Cen Z L, Huang S L, Li R B. 2007. Identification of resistance of rice materials to bacterial leaf streak. Journal of Anhui Agricultural Sciences, 35, 6850-6851, 6853. (in Chinese) [2]Chen C H, Zheng W, Huang X M, Zhang D P, Lin X H. 2006. Major QTL conferring resistance to rice bacterial leaf streak. Agricultural Sciences in China, 5, 101-105. [3]Gnanamanickam S, Brindha Priyadarisini V, Narayanan N, Vasudevan P, Kavitha S. 1999. An overview of bacterial blight disease of rice and strategies for its management. Current Science, 77, 1435-1443. [4]He Y Q, Wen Y H, Huang R R, Zeng X P. 1996. Inheritance of resistance to bacterial leaf streak in hybrid rice. Acta Agriculturae Universitatis Jiangxiensis, 16, 62-65. (in Chinese) [5]Huang D H, Cen Z L, Liu C, Chen Y Z, Ma Z F, Yang L, Wei S L, Liu Y L, Huang S L, Yang X Q, et al. 2008. Identification and genetic analysis of resistance to bacterial leaf streak in wild rice. Journal of Plant Genetic Resources, 9, 11-14. (in Chinese) [6]Khush G S. 1977. The genetic basis of epidemics in agriculture. In: Day R P, ed., Conference on the Genetic Basis of Epidemics in Agriculture. New York Academy of Sciences, New York, USA. pp. 296-308. [7]Khush G S, Bacalangco E, Ogawa T. 1991. A new gene for resistance to bacterial blight form O. longistaminata. Rice Genetics Newsletter, 7, 121. [8]Kosambi D D. 1944. The estimation of map distances from recombination values. Annals of Eugenics, 12, 172-175. [9]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. 1987. MAPMAKER: An interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics, 1, 174-181. [10]Michelmore R W, Paran I, Kesseli R V. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 88, 9828-9832. [11]Murray M G, Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321-4325. [12]Niño-Liu D O, Ronald P C, Bogdanove A J. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Molecular Plant Pathology, 7, 303-324. [13]Nong X M, Liao H D, Liu Z M. 1992. Studies on the methodology for resistance identification of rice cultivar to bacterial leaf streak. Guangxi Plant Protection, 3, 5-9. (in Chinese) [14]Oka H I. 1988. Origin of cultivated rice. In: Developments in Crop Science. Japan Scientific Societies Press, Tokyo. [15]Panaud O, Chen X, McCouch S R. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Molecular and General Genetics, 252, 597-607. [16]Peng S Q, Wei Z S, Mao C X. 1982. Identification of multi resistance of O. meyeriana, O. officinalis and O. sativa f. spantanea growing in Yunan Province. Acta Phytopathologica Sinica, 12, 58-60. (in Chinese) [17]Second G. 1982. Origin of the genetic diversity of cultivated rice (Oryza spp.), study of the polymorphism scored at 40 isozyme loci. The Japanese Journal of Genetics, 57, 25-57. [18]Song W Y, Wang G L, Chen L L, Kim H S, Holsten T, Wang B, Zhai W, Zhu L H, Fauquet C, Ronald P C. 1995. The rice disease resistance gene, Xa-21, encodes a receptor Kinase-like protein. Science, 270, 1084-1086. [19]Tang D Z, Wu W R, Li W M, Lu H R, Worland A J. 2000. Mapping of QTLs conferring resistance to bacterial leaf streak in rice. Theoretical and Applied Genetics, 101, 286-291. [20]Tang J B, Zeng W Y, Wang W M, Ma B T, Liu Y L, Hao J, Xia H G, Li P, Zhu L H. 2001. Genetic analysis and gene mapping of a rice few-tillering mutant in early backcross populations (Oryza sativa L.). Science in China (Series C: Life Sciences), 44, 570-575. [21]Tanksley S D, Nelson J C. 1996. Advanced backcross QTL analysis: a method of the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theoretical and Applied Genetics, 92, 191-203. [22]Vaughan D A. 1994. The wild relatives of rice. In: A Genetic Resources Handbook. IRRI, Los Banos, Phillipines. pp. 13-14. [23]Wang Z Y, Second G, Tanksley S D. 1992. Polymorphism and phylogenetic relationships among species in the genus Oryza as determined by analysis of nuclear RFLPs. Theoretical and Applied Genetics, 83, 565-581. [24]Xu J L, Wang H R, Lin Y Z, Xi Y A. 1997. Studies on the inheritance of the resistance of rice to bacterial streak and bacterial leaf blight. Acta Genetica Sinica, 24, 330-335. (in Chinese) [25]Xu X M, Lin B R, Zeng L X. 1991. Identification of common wild rice germplasm resistant to bacterial leaf streak. Plant Protection, 17, 4-5. (in Chinese) [26]Xu Z G, Qian J M. 1995. Research progress on growing and control measures of bacterial leaf streak in rice. Plant Quarantine, 9, 239-244. (in Chinese) [27]Yang K S, He H H, Chen X R. 2005. Advances in researching on excavation and utilization of the advantageous genes in wild rice. Seed, 24, 92-95. (in Chinese) [28]Zhang X K, Xiao L R, Huang H Q, Zhao B L, Li D H, Yang N J, Zhang X Q. 1992. Resistant identification to bacterial leaf streak of rice resource. Hunan Agricultural Sciences, 2, 33-35. (in Chinese) [29]Zhang H S, Lu Z Q, Zhu L H. 1996. Inheritance of resistance to bacterial leaf streak (Xanthomonas oryzae pv. oryzicola) in four indica rice cultivars. Chinese Journal of Rice Science, 10, 193-196. (in Chinese) [30]Zhang Q, Zhao B Y, Zhao K J, Wang C L, Yang W C, Lin S C, Jue G S, Zhou Y S, Li D Y, Zhu L H. 2000. Identifying and mapping a new gene Xa-23(t) for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Acta Agronomica Sinica, 26, 536-542. (in Chinese) [31]Zhao B Y, Ardales E, Brasset E, Claflin L E, Leach J E, Hulbert S H. 2004. The Rxol/Rbal locus of maize controls resistance reactions to pathogenic and nonhost bacteria. Theoretical and Applied Genetics, 109, 71-79. (in Chinese) [32]Zheng J S, Li Y Z, Fang X J. 2005. Detection of QTL conferring resistance to bacterial leaf streak in rice chromosome 2 (O. sativa L. ssp. indica). Scientia Agricultura Sinica, 38, 1923-1925. (in Chinese) [33]Zhong D B, Luo L J, Guo L B, Yang C S. 1997. Studies on resistance to brown planthopper (BPH) of hybrid between Oryza sativa and Oryza officinalis. Southwest China Journal of Agricultural Sciences, 10, 5-9. (in Chinese) [34]Zhou M H, Xu Z G, Su H, You Y, Zhou Y Z. 1999. Inheritance of resistance to bacterial leaf streak in two indica rice cultivars. Journal of Nanjing Agricultural University, 22, 27-29. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
