|
Special Issue:
水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources
|
|
|
|
| Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
ZHANG Ting*, YOU Jing*, YU Guo-ling, ZHANG Yi, CHEN Huan, LI Yi-dan, YE Li, YAO Wan-yue, TU Yu-jie, LING Ying-hua, HE Guang-hua, LI Yun-feng |
Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China |
|
|
|
|
Abstract The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice (Oryza sativa L.), aberrant-floral spikelet 1 (afs1), which was derived from treatment of Xinong 1B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion (InDel) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04g32510/ LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets.
|
|
Received: 05 August 2019
Accepted: 04 March 2020
|
| Fund: This work was supported by the National Natural Science Foundation of China (31900612 and 31730063), the Fundamental Research Funds for the Central Universities, China (SWU5330500322), the National Key Research and Development Program of China (2017YFD0100202), and the Natural Science Foundation of Chongqing, China (CSTC2017jcyjBX0062). |
|
Corresponding Authors:
Correspondence LI Yun-feng, Tel: +86-23-68250486, E-mail: liyf1980@swu.edu.cn
|
| About author: ZHANG Ting, E-mail: tingwz@163.com; YOU Jing, E-mail: 809217201@qq.com; * These authors contributed equally to this study.
|
Cite this article:
ZHANG Ting, YOU Jing, YU Guo-ling, ZHANG Yi, CHEN Huan, LI Yi-dan, YE Li, YAO Wan-yue, TU Yu-jie, LING Ying-hua, HE Guang-hua, LI Yun-feng.
2020.
Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.). Journal of Integrative Agriculture, 19(4): 921-930.
|
Bowman J L, Smyth D R, Meyerowitz E M. 1991. Genetic interactions among floral homeotic genes of Arabidopsis. Development, 112, 1–20.
Gao X, Liang W, Yin C, Ji S, Wang H, Su X, Guo C, Kong H, Xue H, Zhang D. 2010. The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development. Plant Physiology, 153, 728–740.
Jeon J S, Jang S, Lee S, Nam J, Kim C, Lee S H, Chung Y Y, Kim S R, Lee Y H, Cho Y G, An G. 2000. leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development. The Plant Cell, 12, 871–884.
Jin Y, Luo Q, Tong H N, Wang A J, Cheng Z J, Tang J F, Li D Y, Zhao X F, Li X B, Wan J M, Jiao Y L, Chu C C, Zhu L H. 2011. An AT-hook gene is required for palea formation and floral organ number control in rice. Developmental Biology, 359, 277–288.
Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174–181.
Lee D Y, An G. 2012. Two AP2 family genes, supernumerary bract (SNB) and Osindeterminate spikelet 1 (OsIDS1), synergistically control inflorescence architecture and floral meristem establishment in rice. The Plant Journal, 69, 445–461.
Lee D Y, Lee J, Moon S, Park S Y, An G. 2007. The rice heterochronic gene SUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meristem. The Plant Journal, 49, 64–78.
Li A, Zhang Y, Wu X, Tang W, Wu R, Dai Z, Liu G, Zhang H, Wu C, Chen G, Pan X. 2008. DH1, a LOB domain-like protein required for glume formation in rice. Plant Molecular Biology, 66, 491–502.
Li H, Liang W, Jia R, Yin C, Zong J, Kong H, Zhang D. 2010. The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Research, 20, 299–313.
Lin X, Wu F, Du X, Shi X, Liu Y, Liu S, Hu Y, Theissen G, Meng Z. 2014. The pleiotropic SEPALLATA-like gene OsMADS34 reveals that the ‘empty glumes’ of rice (Oryza sativa) spikelets are in fact rudimentary lemmas. New Phytologist, 202, 689–702.
Liu H, Guo S, Xu Y, Li C, Zhang Z, Zhang D, Xu S, Zhang C, Chong K. 2014. OsmiR396d-regulated OsGRFs function in floral organogenesis in rice through binding to their targets OsJMJ706 and OsCR4. Plant Physiology, 165, 160–174.
Liu M, Li H, Su Y, Li W, Shi C. 2016. G1/ELE functions in the development of rice lemmas in addition to determining identities of empty glumes. Frontiers in Plant Science, 7, 1006.
Lombardo F, Yoshida H. 2015. Interpreting lemma and palea homologies: A point of view from rice floral mutants. Frontiers in Plant Science, 6, 61.
Luo Z, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. 2007. Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10(t), in rice (Oryza sativa L.). Genome, 50, 811–817.
Michelmore R W, Paran I, Kesseli R V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 88, 9828–9832.
Murray M G, Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321–4326.
Ohmori S, Kimizu M, Sugita M, Miyao A, Hirochika H, Uchida E, Nagato Y, Yoshida H. 2009. MOSAIC FLORAL ORGANS1, an AGL6-Like MADS box gene, regulates floral organ identity and meristem fate in rice. The Plant Cell, 21, 3008–3025.
Ren D Y, Li Y F, Zhao F M, Sang X C, Shi J Q, Wang N, Guo S, Ling Y H, Zhang C W, Yang Z L, He G H. 2013. MULTI-FLORET SPIKELET1, which encodes an AP2/ERF protein, determines spikelet meristem fate and sterile lemma identity in rice. Plant Physiology, 162, 872–884.
Ren D Y, Xu Q K, Qiu Z N, Cui Y J, Zhou T T, Zeng D L, Guo L B, Qian Q. 2019. FON4 prevents the multi-floret spikelet in rice. Plant Biotechnology Journal, 17, 1007–1009.
Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z , Liu Y S, He G H. 2012. CHIMERIC FLORAL ORGANS1, Encoding a monocot-specific MADS box protein, regulates floral organ identity in rice. Plant Physiology, 160, 788–807.
Sun Q, Zhou D X. 2008. Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. Proceedings of the National Academy of Sciences of the United States of America, 105, 13679–13684.
Tabuchi H, Zhang Y, Hattori S, Omae M, Shimizu-Sato S, Oikawa T, Qian Q, Nishimura M, Kitano H, Xie H, Fang X H, Yoshida H, Kyozuka J, Chen F, Sato Y. 2011. LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems. The Plant Cell, 23, 3276–3287.
Tanaka W, Toriba T, Ohmori Y, Yoshida A, Kawai A, Mayama-Tsuchida T, Ichikawa H, Mitsuda N, Ohme-Takagi M, Hirano H Y. 2012. The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet. The Plant Cell, 24, 80–95.
Wang K J, Tang D, Hong L L, Xu W Y, Huang J, Li M, Gu M H, Xue Y B, Cheng Z K. 2010. DEP and AFO regulate reproductive habit in rice. PLoS Genetics, 6, e1000818.
Xiang C Y, Liang X X, Chu R Z, Duan M, Cheng J P, Ding Z Q, Wang J F. 2015. Fine mapping of a palea defective 1 (pd1), a locus associated with palea and stamen development in rice. Plant Cell Reports, 34, 2151–2159.
Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z, He G H, Zhu L H. 2009. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. The Plant Journal, 59, 789–801.
Yan D, Zhang X, Zhang L, Ye S, Zeng L, Liu J, Li Q, He Z. 2015. Curved chimeric palea 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. The Plant Journal, 82, 12–24.
Yoshida H, Nagato Y. 2011. Flower development in rice. Journal of Experimental Botany, 62, 4719–4730.
Yuan Z, Gao S, Xue D W, Luo D, Li L T, Ding S Y, Yao X, Wilson Z A, Qian Q, Zhang D B. 2009. RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiology, 149, 235–244.
Zeng D D, Qin R, Alamin M, Liang R, Yang C C, Jin X L, Shi C H. 2016. DBOP specifies palea development by suppressing the expansion of the margin of palea in rice. Genes & Genomics, 38, 1095–1103.
Zhang J, Cai Y, Yan H G, Jin J, You X M, Wang L, Kong F, Zheng M, Wang G X, Jiang L, Zhang W W, Wan J M. 2018. A critical role of OsMADS1 in the development of the body of the palea in rice. Journal of Plant Biology, 61, 11–24.
Zheng H, Zhang J, Zhuang H, Zeng X Q, Tang J, Wang H L, Chen H, Li Y, Ling Y H, He G H, Li Y F. 2019. Gene mapping and candidate gene analysis of multi-floret spikelet 3
(mfs3) in rice (Oryza sativa L.). Journal of Integrative Agriculture, 18, 2–10.
Zheng M, Wang Y, Wang Y, Wang C, Ren Y, Lv J, Peng C, Wu T, Liu K, Zhao S, Liu X, Guo X, Jiang L, Terzaghi W, Wan J. 2015. DEFORMED FLORAL ORGAN1 (DFO1) regulates floral organ identity by epigenetically repressing the expression of OsMADS58 in rice (Oryza sativa). New Phytologist, 206, 1476–1490. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
