Loading...

Table of Content

    15 June 2011, Volume 44 Issue 12
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Functional Marker Mapping of Protein Phosphatase 2A Structural Subunit Gene TaPP2Aa in Common Wheat
    WANG Zhi-lan;MAO Xin-guo; LIAng; CHANG Xiao-ping; LIU Hui-min; JING Rui-lian
    Scientia Agricultura Sinica. 2011, 44(12):  2411-2421 .  doi:10.3864/j.issn.0578-1752.2011.12.001
    Abstract ( 1409 )   PDF (876KB) ( 618 )   Save
    Related Articles | Metrics

    【Objective】The purpose of the present research was to develop functional markers of protein phosphatase 2A structural subunit gene PP2Aa in common wheat (Triticum aestivum L.) for marker assisted selection in breeding of drought tolerance. 【Method】Based on the single nucleotide polymorphism (SNP) in the sequences of PP2Aa gene in common wheat and its wild relative species, three genome-specific primer pairs and six allele-specific primer pairs were designed. The Chinese Spring nulli-tetrasomic lines, a recombinant inbred line (RIL) population (Opata 85 × W7984) and a doubled haploid (DH) population (Hanxuan 10 hao× Lumai 14) were used for genetic location and mapping of TaPP2Aa. 【Result】The TaPP2Aa gene was located on chromosomes 5A, 5B and 5D. TaPP2Aa-B was mapped in the intervals between markers Xwg909 and Xgwm67 on chromosome 5B in RIL population, 4.0 cM and 3.6 cM from the flanking markers, and between markers Xgwm234 and WMC363 on chromosome 5B in DH population, 7.5 cM from WMC363, 3.6 cM and 11.4 cM from the common marker Xgwm67 in two populations, respectively. TaPP2Aa-D was mapped in the interval between markers Xcmwg770 and Xbarc205 on chromosome 5D in RIL population, 9.8 cM and 10.0 cM from them. 【Conclusion】The present study mapped the TaPP2Aa on the homoeologous chromosomes 5A, 5B and 5D.Through comparative analysis with main effect QTLs for stress resistance in DH and RIL genetic populations, the genetic linkage between TaPP2Aa and stress-resistant QTLs was identified. The functional markers developed for TaPP2Aa-B and TaPP2Aa-D can be used for marker assisted selection to enhance the efficiency of wheat stress-tolerant improvement.

    Construction of a Molecular Linkage Map Using SRAP and Mapping of Three Qualitative Traits in Corchorus olitorius L.
    CHEN Hui; CHEN Mei-xia; TAO Ai-fen; ZHANG Guang-qing; XU Jian-tang; QI Jian-min; FANG Ping-ping
    Scientia Agricultura Sinica. 2011, 44(12):  2422-2430 .  doi:10.3864/j.issn.0578-1752.2011.12.002
    Abstract ( 893 )   PDF (586KB) ( 595 )   Save
    Related Articles | Metrics

    【Objective】Jute (Corchorus olitorius L.) is one of the most economically important crops for fiber production. The present study was aimed to construct a molecular linkage map of jute and locate important qualitative traits, which will provide essential tools for investigating genomic structure and evolution, facilitating QTL mapping, marker-assisted selection and map-based cloning of important gene. 【Method】In this study, a population of F2 generation including 187 individual plants was derived from the cross between Corchorus aestuans L. (wild species) and wild leaf jute (cultivated species). A total of 513 sequence-related amplified polymorphisms (SRAP) primer pairs were used in constructing the molecular genetic linkage map and mapping 3 qualitative traits including color of stipule, color of stipe, and color of leaf margin.【Result】This genetic linkage map containing 10 linkage groups (LGs) was constructed using 122 SRAP loci and these 3 morphological markers, the map covered 2 231.9 cM with the average marker interval of 17.86 cM, every linkage group included 2-38 markers.【Conclusion】All the markers are distributed evenly among the linkage groups without clustering of loci. SRAP markers may be a better type of markers in map construction.

    Stability of Stress-Induced DNA Methylation in Plant

    PENG Ha; XI Ting; ZHANG Jing; WU Xian-jun
    Scientia Agricultura Sinica. 2011, 44(12):  2431-2438 .  doi:10.3864/j.issn.0578-1752.2011.12.003
    Abstract ( 807 )   PDF (282KB) ( 1080 )   Save
    Related Articles | Metrics

    DNA methylation is an important mechanism for plant stress response. It regulates traits such as flowering, yielding and resistance, which are closely related to the stability of species or variety. Most of these related DNA methylation alterations are uniform within population, somewhat heritable between generations and therefore, only slightly at random. Under the biotic stresses, the methylation levels are decreased at the resistance gene loci while increased on genome-wide level. This is consistent with the mechanisms of genetics and evolution, and therefore, should not be at random. Such a phenomenon can be directly or indirectly proved in biotic stresses of tobacco and Arabidopsis, or in abiotic stresses such as cold, salt, heavy metal and polyploidization. The causes of random variation in methylation analysis and the control measures were also discussed.
    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Dynamic Differences of the RVA Profile and Gel Consistency in Two Rice Varieties with Similar Apparent Amylose Content During Grain Filling
    CAI Yi-xia ; LIU Chun-xiang; WANG Wei; ZHANG Hong-xi; ZHANG Zu-jian;YANG Jing; TANG Han-zhong
    Scientia Agricultura Sinica. 2011, 44(12):  2439-2445 .  doi:10.3864/j.issn.0578-1752.2011.12.004
    Abstract ( 836 )   PDF (387KB) ( 693 )   Save
    Related Articles | Metrics

    【Objective】 This study was performed to investigate the reason of the difference in the two rice varieties with similar apparent amylose content (AAC) by analyzing the dynamic formation of rice quality. 【Method】 Two rice cultivars Wuyujing3 and 30you917 with similar apparent amylose content were used as materials. The dynamic changes of gel consistency and RVA profile of the developing grains during the quality formation were studied. 【Result】 The differences were obviously found in the dynamic changes of amylose content, gel consistency and RVA profile of the developing grains. The difference in dynamic change of amylose content in two varieties from 5d to 15d after anthesis was significant, while the contents of amylose were nearly equal at late grain-filling stage. Gel consistency and RVA profile between the two varieties varied in the grain-filling period. The gel consistence of Wuyujing3 was softer than that of 30you917 at 5-10 d after anthesis, while harder than that of 30you917 at 20-40 d after anthesis. During the determination of RVA profile, Wuyujing3 represented higher stickiness such as big breakdown, small positive setback and low pasting temperature compared to 30you917. 【Conclusion】 These results suggested that the differences in the dynamic formation of the main quality indices during grain filling were the representation of the diversities of rices with similar apparent amylose content. It was Wuyujing3 that provided with higher peak viscosity, breakdown and lower positive setback during the formation of rice kernel and had high cooking and eating qualities.

    Studies on the Characteristics of Nitrogen Fertilizer Utilization in Summer Maize Based on Techniques of Soil Column and 15N-label
    WU Yong-cheng; WANG Zhi-min; ZHOU Shun-li
    Scientia Agricultura Sinica. 2011, 44(12):  2446-2453 .  doi:10.3864/j.issn.0578-1752.2011.12.005
    Abstract ( 775 )   PDF (382KB) ( 719 )   Save
    Related Articles | Metrics

    【Objective】For quantitatively studying the characteristics of nitrogen (N) fertilizer utilization and the fate of fertilizer N in summer maize, an experiment was conducted in Wuqiao Experimental Station of China Agricultural University in 2005. 【Method】The experiment design was employed with four N fertilizer application rates (0, 90, 180, 270 kgN·hm-2) and three replications in each treatment, and was carried out by the techniques of soil column and 15N-label under the condition of artificial weather shed. 【Result】Application of N fertilizer significantly improved the dry matter, grain yield, total N uptake rate of maize plant and grain. There was no significant difference in PNUE (physiological N fertilizer use efficiency) but showed a tendency of rising firstly and falling then with the increase of N fertilizer applied. AENF (agronomic efficiency of applied N-fertilizer) and ANRE (apparent N fertilizer recovery efficiency) decreased significantly with the increase of N fertilizer applied. The recovery ratio of 15N was 41.2%-47.8% in maize season and the percentage of residual 15N in 2 m-depth soil body was 40.7%-47.5%. Residual 15N rates of different soil layers increased significantly with the increase of N fertilizer applied (P<0.05) and decreased obviously with the add of soil layer depth. Loss rates of 15N increased significantly with the increase of N fertilizer applied and total 15N loss ratio was 9.0%-13.1%. 【Conclusion】Proper rates of N applied(90-180 kg·hm-2)would be favorable to improve grain yield and N use efficiency, reduce residual 15N rates in soil body (especially in deep-soil layer) and therefore lighten the environment pressure.

    PLANT PROTECTION
    Isolation and Expression Profiles of Rice WRKY30 Induced by Jasmonic Acid Application and Fungal Pathogen Infection
    PENG Xi-xu; HU Yao-jun; TANG Xin-ke; ZHOU Ping-lan; DENG Xiao-bo; WANG Hai-hua
    Scientia Agricultura Sinica. 2011, 44(12):  2454-2461 .  doi:10.3864/j.issn.0578-1752.2011.12.006
    Abstract ( 899 )   PDF (513KB) ( 656 )   Save
    Related Articles | Metrics

    【Objective】The objective of this study is to isolate rice WRKY30 and provide not only a foundation for elucidating the roles of WRKY transcription factors in the regulation of defense responses in rice, but also gene sources for reasonable utilization of pathogen-resistance and genetic modification in rice breeding. 【Method】RT-PCR was used to amplify the full cDNA length of WRKY30 including its largest open reading frame (ORF). Bioinformatical tools were employed to analyze its sequence and coding protein. Copies of WRKY30 in the rice genome were determined by Southern hybridization, and the organ-specific expression characteristics and induced expression profiles under pathogen challenges, hormone treatments and abiotic stresses were investigated by Northern blot or RT-PCR. 【Result】The WRKY30 contained an entire ORF in length of 2 022 bp and was predicted to encode a protein of 674 amino acid residues consisting of two WRKY domains each with a zinc finger motif of C2H2 and a nuclear localization signal, belonging to the WRKY subgroup I. WRKY30 shared high amino acid sequence identity with those from monocots such as wheat (Hordeum vulgare), Sorghum bicolor and Brachypodium sylvaticum. WRKY30 exists as a single copy in the rice genome. The transcript levels of WRKY30 were relatively high in stem, leaf and grain, next were root and panicle, and the least was flower. WRKY30 was rapidly induced in response to challenges with Magnaporthe grisea and Rhizoctonia solani, and also induced by exogenous salicylic acid (SA) and methyl jasmonate (MeJA), two important defense-related hormones. However,ethephon (ET) exerted no obvious effects on its expression. By contrast, abscisic acid inhibited the transcription of WRKY30. On the hand, wounding lead to upregulation of WRKY30 transcription, whereas there were no significant effects on its expression subjected to other abiotic stresses such as cold, high salt and dehydration. 【Conclusion】WRKY30 may be involved in the regulation of rice defense responses to blast and sheath blight fungi through SAand JA, but not ET-dependent signaling pathways.

    Nitrobacteria Community and Diversity in the Rhizosphere Soil of Mature Tobacco
    LI Xiao-lin; GU Yun-fu; ZHANG Xiao-ping; TU Shi-hua; WU Ren-jun
    Scientia Agricultura Sinica. 2011, 44(12):  2462-2468 .  doi:10.3864/j.issn.0578-1752.2011.12.007
    Abstract ( 802 )   PDF (431KB) ( 573 )   Save
    Related Articles | Metrics

    【Objective】The objective of the study is to evaluate the community structure and diversity of nitrobacteria community in rhizosphere and non-rhizosphere soils at tobacco mature stage. 【Method】Five tobacco rhizosphere soil samples and five non-rhizosphere soil samples were collected from the main tobacco-producing areas of Panzhihua Yanbian County and analyzed by PCR-DGGE technology. 【Result】DGGE analysis showed that nitrobacteria community structure and diversity were different among different soils. Overall, the rhizosphere diversity index (H), abundance (S) and evenness (EH) were not all higher than those of non-rhizosphere soil, which might be related to rhizosphere secretion at tobacco mature stage which had an inhibitory effect on nitrobacteria. Moreover, the nitrobacteria community structures in different soil samples had a great difference. The recovery DGGE strip series showed that most of them were similar to Nitrospira sp. of the extreme environment such as glacier, or dissolution rock, while small of them were closely related to uncultivated nitrobacteria.【Conclusion】The diversity of nitrobacteria in the mature tobacco rhizosphere soils was higher than that of non-rhizsphere soils.

    Rapid Screen Formulation for 5% Hexaconazole Suspension Concentrate Using Triangle-coordinate Figure
    ZHANG Da-xia; LIU Feng; ZHANG Gui-sen; CHEN-Bo; YANG Xiao-nan
    Scientia Agricultura Sinica. 2011, 44(12):  2469-2475 .  doi:10.3864/j.issn.0578-1752.2011.12.008
    Abstract ( 986 )   PDF (395KB) ( 692 )   Save
    Related Articles | Metrics

    【Objective】The objective of this study is to build a rapid screening recipe of hexaconazole suspension concentrate. 【Method】The suspension system was divided into wetting dispersants and antisettling agents by using the method, and then used, respectively, to the triangle-coordinate figure. In order to shorten the screening period, the method replaced grinding with shearing to obtain samples, and the important and quality performance indices such as viscosity, water separation ratio, suspensibility and dispersion were selected. The optimum recipe was obtained by further screening, based on determining a better region in the triangle rectangular coordinate.【Result】The optimum formulation of 5% hexaconazole SC was determined: 5% hexaconazole, 0.5% NP-10, 1% 1602,3.5% VSI-B88, 0.025% XG, 2% magnesium aluminum silicatenf, 2.5% Na-Ben, 3% ethylene glycol and fill water to 100%. Throughout the selection process, 1 040 g sample and 13 hours were consumed, which was more efficient than the conventional method. That replaced grinding with shearing could save time 3/4 and save 1/2 or more raw materials.【Conclusion】The technological assay established in this study is a rapid, high efficient and simple tool for screening the formulation of SC.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Effects of Topsoil Water and Nitrogen Control on the Movement and Residual Effect of Nitrate Accumulated in Deep Soil Profile
    DONG Xian-xian;LIU Chen-chen;ZHANG Li-juan;;YUAN Li-jin;JU Xiao-tang;LIU Xin-yu
    Scientia Agricultura Sinica. 2011, 44(12):  2476-2483 .  doi:10.3864/j.issn.0578-1752.2011.12.009
    Abstract ( 746 )   PDF (365KB) ( 658 )   Save
    Related Articles | Metrics

    【Objective】The purpose of this study was to study the effects of topsoil water and nitrogen control on the movement and residual effect of nitrate accumulated in deep soil profile in north China. 【Method】The injection technique of labeled NO3--N was adopted in a field micro-plot experiment. Nitrogen applied at 0 and 150 kgN·hm-2 N and two irrigation modes were designed. The four treatments were N0W1, N0W2, N150W1, N150W2. The labeled NO3--N was injected in 110 cm soil profile.【Result】Under this condition, the distribution of residual amount of 15N in each soil profile after wheat harvest was: N0W2<N150W2<N150W1<N0W1. The labeled 15N moved at vertical distance. It moved 30 cm to the top soil profile and 50 cm to the lower. In addition to N0W2, the other treatments with the cumulative peak position than the mark down 30 cm; After maize harvest, most 15N was detected in 100-160 cm. The residues of 15N of N0W1 reduced significantly; The utilizations of residual 15N by maize were: 5.5%, 2.2%, 1.7% and 1.5%, and N0W1>N150W2>N150W1>N0W2. Maize roots were distributed in 0-20 cm, and N treatment was higher than non-fertilizer treatment, topsoil water and nitrogen control affected root development in the middle and lower.【Conclusion】Less nitrogen and reasonable water stress promoted the growth of maize roots in subsoil layers which accelerated the absorption of nitrate accumulated in deep soil profile. Nitrogen does not promote the use of maize residue N, nitrogen and traditional irrigation of plow layer will enhance the accumulation of nitrate in the deep, and thus resulting in the security threat to groundwater.

    Effects of Long-term Fertilization on Carbon and Nitrogen in Yellow Clayey Soil and Its Nitrogen Utilization
    JI Jian-hua; LIU Xiu-mei; LI Zu-zhang; LIU Yi-ren; HOU Hong-qian; LIU Guang-rong; LUO Qi-xiang
    Scientia Agricultura Sinica. 2011, 44(12):  2484-2494 .  doi:10.3864/j.issn.0578-1752.2011.12.010
    Abstract ( 983 )   PDF (403KB) ( 565 )   Save
    Related Articles | Metrics

    【Objective】Under the same nitrogen application condition, the variation of carbon and nitrogen in yellow clayey soil and its relationship with NO3--N accumulation under long-term fertilization were studied to illustrate the main factors affecting the NO3--N accumulation. 【Method】The changes of soil organic carbon(SOC), total nitrogen(TN), microbial biomass carbon(SMBC), microbial biomass nitrogen(SMBN), recovery efficiency of nitrogen and the correlation among accumulated amount of NO3--N in a yellow clayey soil after fertilization were analyzed based on a 25 year long - term fertilizer experiment in yellow clayey soil in Nanchang County, Nanchang, China. In an experiment carried out in 1984 at this site, 8 different treatments were designed in an early rice-late rice rotation system. The treatments were no fertilizer(CK), nitrogen fertilizers deficiency (PK),potassium fertilizers deficiency (NP), phosphorus fertilizers deficiency (NK), balanced chemical fertilizers application (NPK), 70% chemical fertilizers substituted by 30% organic manure in balanced chemical fertilizers application (70F+30M), 50% chemical fertilizers substituted by 50% organic manure in balanced chemical fertilizers application (50F+50M), 30% chemical fertilizers substituted by 70% organic manure in balanced chemical fertilizers application (70F+30M).【Result】Two main results were found from this experiment. First, in the double rice-cropping syetem areas, combined application of organic manure and chemical fertilizers were better than inorganic fertilizers for improving the SOC, TN, available N, SMBC and SMBN, in which combined application of high amount (70%) or low amount (30%) of organic manure was the best. Second, the content and accumulated amount of NO3--N of the combined high amount or low amount of organic fertilizer treatments were more than chemical fertilizers in the soil profile under the same nitrogen condition. The content and accumulated amount of NO3--N of the same ratio of organic manure
    combined with chemical fertilizers treatments which greatly reduced the accumulated amount of NO3--N in the soil were equal with no fertilization. 【Conclusion】High amount and the same ratio of organic manure combined with chemical fertilizers is the best to improve soil quality and decrease the accumulated amount of NO3--N in soil, respectively. In consideration of soil quality and environmental protection, the ratio of organic manure combined with chemical fertilizers should be used in the region. Factors affecting NO3--N accumulation are SOC, TN, SMBC and SMBN under the same nitrogen conditions, and SMBC is the most important and direct factor.

    HORTICULTURE
    Effects of 24-Epibrassinolide on Carbohydrate Metabolism and Enhancement of Tolerance to Root-Zone Hypoxia in Cucumber (Cucumis sativus L.)
    KANG Yun-yan ; YANG Xian ; GUO Shi-rong ; ZHANG Ying-ying
    Scientia Agricultura Sinica. 2011, 44(12):  2495-2503 .  doi:10.3864/j.issn.0578-1752.2011.12.011
    Abstract ( 740 )   PDF (496KB) ( 702 )   Save
    Related Articles | Metrics

    【Objective】The aim of the work was to study the effects of 24-epibrassinolide (EBR) added to nutrient solution on carbohydrate metabolism of cucumber (Cucumis sativus L.) under root-zone hypoxia. 【Method】Seedlings of a relatively hypoxia-resistant cultivar, ‘Lübachun No.4’, and a relatively hypoxia-sensitive cultivar, ‘Zhongnong No.8’, were hydroponically grown for 8 days in normoxic and hypoxic nutrient solutions with and without addition of EBR at 1 μg L-1. And the effects of EBR on contents of carbohydrates in leaves and roots and activities of glycolytic enzymes in the roots were investigated. 【Result】Application of EBR to normoxic nutrient solution promoted the accumulation of total soluble sugars in leaves and roots, but had no effects on activities of glycolytic enzymes in the roots. Changes in contents of starch and glucose, and activities of acid invertase and ATP-dependent phosphofructokinase enzymes in the roots showed obviously different between two genotypes cucumber in response to root-zone hypoxia. EBR added to hypoxic nutrient solution caused an increase in the concentrations of fructose and sucrose and activities of major glycolytic enzymes in the roots, but exerted little influence on carbohydrate concentrations in the leaves. 【Conclusion】These results suggest that EBR added to hypoxic nutrient solution may stimulate the photosynthate allocation down to roots and activities of major glycolytic enzymes, and eventually enhanced tolerance of cucumber plants to root-zone hypoxia.

    Development of Plant-Derived Organic Fertilizers and Their Effect on the Young Pear Trees
    ZHAO Ling-ling; ZHANG Jie; LIU Yan; HUO Ying; LI Zhen-ru; YAO Yun-cong
    Scientia Agricultura Sinica. 2011, 44(12):  2504-2514 .  doi:10.3864/j.issn.0578-1752.2011.12.012
    Abstract ( 767 )   PDF (436KB) ( 570 )   Save
    Related Articles | Metrics

    【Objective】The purpose of this paper is to devise and select valuable botanical organic fertilizers with good effect on young pear trees growing in sandy soil. 【Method】Thinking of maximum nutrition, single element and balance of all the nutrition elements, composition was as follows: Glycine maa Merrill, Phaseolus aureus Rosa, Trifolium repens L., Medicago sativa L., Astragalus adsurgens Pall were selected as basic materials for organic fertilizer, 5(T1-T5) out of 32 decomposed manures designed by orthogonal design were selected in this paper, using farmyard manure (T6) and without manure (T7) as controls. The nutrition condition of soil and young pear tree leaves was investigated. 【Result】Compared with CK(No fertilizer and manure), fertilizer processing could improve the organic matter content of the soil, also with increased mineral elements, the soil moisture remained higher than that of CK, the height and diameter of young pear tree enhanced, the accumulation of dry matter was more than that of control. At the same time, the net photosynthesis rate and water use efficiency were better than that of control. T1 had a significant effect on the content of soil K, N, exchangeable Ca, available Fe, organic matter content, the same effects were also found on the leaf Ca and Fe content. While taking soil available Cu, the leaf K, Cu content and leaf dry matter as all, T2 was the best of all treatments. As for the content of soil available K, Mn, leaf Mn and photosynthetic rate, T3 was the best among all the treatments. T4 had good effect on soil exchangeable Mg, available Zn, and the highest leaf N, Mg and Zn content, the height of young pear tree was found in this treatment.【Conclusion】The tested organic fertilizers had good effect on improving soil organic matter content, remaining higher soil moisture, keeping balance of all elements, soil capacity of nutrient supplying, all those contributed to the better growth condition of the young pear tree, especially T1, T2.

    Cloning and Expression Analysis of miR156-Targeted SPL9 Gene from Strawberry
    ZHAO Xiao-chu; LI He; DAI Hong-yan; LIU Yue-xue; MA Yue; ZHANG Zhi-hong
    Scientia Agricultura Sinica. 2011, 44(12):  2515-2522 .  doi:10.3864/j.issn.0578-1752.2011.12.013
    Abstract ( 1003 )   PDF (951KB) ( 875 )   Save
    Related Articles | Metrics

    【Objective】The aim of this study was to clone the full length cDNA of the gene encoding SQUAMOSA promoter binding protein-like 9 (SPL9) from strawberry, to analyze its expression level at different growth stages of strawberry and the expression relationship between SPL9 and miR156, and preliminarily to elucidate the role of SPL9 gene in strawberry plant development.【Method】The fragment of SPL9 gene was amplified from strawberry leaves by RT-PCR with the degenerate primers designed according to the sequences of Malus SPL genes. The full-length cDNA of SPL9 gene was obtained with RACE. Real time RT-PCR was used to analyze the expression levels of SPL9 and miR156 in strawberry leaves.【Result】The full-length cDNA sequence of SPL9 was cloned from strawberry (Fragaria×ananassa) cultivar ‘Allstar’ and the gene product was designated as Fragaria×ananassa SPL9 (FaSPL9). The CDS length of FaSPL9 is 1143bp that encoded a predicted protein of 381 amino acid residues. The amino acid identity compared with Arabidopsis thaliana AtSPL9, Vitis vinifera VvSPL9, Poncirus trifoliate PtSPL9, Malus domestica MdSPL9 and Zea mays ZmSPL9 was 40.30%, 64.57%, 56.09%, 70.33%, and 38.35%, respectively. There are highly conserved SBP structure domain and two-way nuclear localization signal KRXXXRRRK in FaSPL9. The nucleotide sequence of FaSPL9 includes the target site of miR156. The real time RT-PCR result showed that the expression levels of FaSPL9 changed at the different development stages of strawberry plants, and the expression of FaSPL9 was strikingly complementary with the expression of miR156.【Conclusion】FaSPL9 gene was cloned from strawberry. As the target gene of miR156, FaSPL9 may play an important role in regulating the growth and development of strawberry plants.

    STORAGE·FRESH-KEEPING·PROCESSING
    Effects of Cold Shock Treatments on Chilling Injury and Antioxidative Defense System of Pepper
    GU Hui; GONG De-qiang; ZHU Shi-jiang; XIE Jiang-hui; ZHANG Lu-bin
    Scientia Agricultura Sinica. 2011, 44(12):  2523-2530 .  doi:10.3864/j.issn.0578-1752.2011.12.014
    Abstract ( 769 )   PDF (415KB) ( 640 )   Save
    Related Articles | Metrics

    【Objective】In this study, the effects of pre-storage cold shock treatment at 0℃ on storability of pepper kept at 8℃ in relation to physiological mechanisms were investigated. 【Method】Peppers were treated with cold air at 0℃ for 4 h and 12 h, respectively, and then stored at 8℃, chilling injury index, cell membrane permeability, and antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were analyzed. 【Result】Cold shock treatment at 0℃ for 4 h significantly alleviated chilling injury and disease incidence, delayed the increase of cell membrane permeability of pepper pericarps, enhanced the enzyme activities of SOD, CAT and POD, reduced the contents of H2O2 in the early days of low temperature storage, and increased the contents of soluble proteins and heat-stable proteins in pepper pericarps. However, cold shock treatment at 0℃ for 12 h aggravated pepper chilling injury and diseases occurrence, and resulted in opposite physiological effects in comparison with that for 4 h. 【Conclusion】It is suggested that the enhanced storability of pepper at low temperature by 4 h of cold shock treatment at 0℃ was related to the activated defense system, which was beneficial to maintaining the integrity of cell membrane.

    Cluster Analysis of the Main Spoilage Bacteria During Storage in Chilled Pork
    LI Miao-yun; ZHAO Gai-ming; ZHANG Jian-wei; ZHANG Qiu-hui; ZHU Ying-ju
    Scientia Agricultura Sinica. 2011, 44(12):  2531-2537 .  doi:10.3864/j.issn.0578-1752.2011.12.015
    Abstract ( 1054 )   PDF (343KB) ( 988 )   Save
    Related Articles | Metrics

    【Objective】The experiment was to determine the key spoilage bacteria in whole spoilage effect during storage in order to provide a theoretical basis for microbial control and shelf life extension. 【Method】Single and mixed bacteria were inoculated to chilled pork surface. The chilled pork spoilage qualities at different storage times were determined by tray-packaged at 4℃. The quality indexes of different groups were studied by cluster analysis. 【Result】The single and mixed bacteria in chilled tray-packaged pork were clustered together into three sorts which one was single bacterium and the others were different mixed bacteria. A group of mixed bacteria (including Aeromonas hydrophila, Brochothrix thermosphacta, Enterobacter and Pseudomonas) and naturally contaminative microorganisms were clustered together in one sort. The heredity distance was the least and its Squared Euclidean distance was 45.797. Therefore, it was not one type microorganism but the combined spoilage bacteria to meat spoilage quality.【Conclusion】Aeromonas hydrophila was the key bacterium for spoilage in whole spoilage quality due to mixed spoilage bacteria containing Aeromonas hydrophila, Brochothrix thermosphacta, Enterobacter and Pseudomonas during storage in chilled pork.

    Calpains in Chicken Muscle and Comparison of Calcium Sensitivity with Other Animals
    HUANG Ming; XUE Mei; HUANG Ji-chao; XU Bao-cai; GUO Lin; XU Xing-lian; ZHOU Guang-hong
    Scientia Agricultura Sinica. 2011, 44(12):  2538-2544 .  doi:10.3864/j.issn.0578-1752.2011.12.016
    Abstract ( 1052 )   PDF (416KB) ( 557 )   Save
    Related Articles | Metrics

    【Objective】The objective of this experiment was to investigate the variety and characteristics of calpains in chicken muscle and compare calcium sensitivity of calpains with other animals. 【Method】Calpains were extracted from chicken breast muscle and purified by dialysis after ammonium sulfate precipitation. After slaughter immediately, calpains were also extracted from fish, duck, rabbit, pig and bovine, respectively, and calpain activity was detected by casein zymography. The gels were incubated with an increasing concentration of 0 mmol·L-1, 0.01 mmol·L-1, 0.03 mmol·L-1, 0.05 mmol·L-1, 0.10 mmol·L-1, 0.50 mmol·L-1 and 5.00 mmol·L-1 CaCl2 in order to test their calcium sensitivity. 【Result】μ-calpain of chicken and duck had a lower electrophoretic mobility than that from rabbit, pork and beef. When the calcium concentration increased from 0.01 mmol·L-1 to 0.03 mmol·L-1, the activity of μ-calpain in rabbit, pork and beef was enhanced, while the activity of chicken and duck had no change (P>0.05), indicating that μ-calpain in bird species had a greater calcium sensitivity than that from mammalian; another calpain in chicken muscle with a calcium sensitivity between that of mammalian μ-calpain and the m-calpain was named μ/m-calpain. The μ/m-calpain in chicken and duck accounted for 67.4% and 88.4% of the total activity.【Conclusion】Calpains in chicken muscle have a greater calcium sensitivity than that from mammalian.

    ANIMAL SCIENCE·RESOURCE INSECT
    Comparative Proteomic Analysis of the Changes of Milk Protein Associated with Somatic Cell Counts
    YANG Yong-xin; WANG Jia-qi; BU Deng-pan; LI Shan-shan; ZHOU Ling-yun
    Scientia Agricultura Sinica. 2011, 44(12):  2545-2552 .  doi:10.3864/j.issn.0578-1752.2011.12.017
    Abstract ( 942 )   PDF (689KB) ( 863 )   Save
    Related Articles | Metrics

    【Objective】The objective of the study is to investigate the changes of the milk protein with low somatic cell counts, medium somatic cell counts, high somatic cell counts and clinical mastitis.【Method】Milk proteins were examined using an integrated proteomic approaches consisting of protein separation by two-dimensional gel electrophoresis, stained with Coomassie Blue G-250 solution, and protein identification by HPLC coupled with tandem ion trap spectrometry.【Result】Albumin and caseins variant were increased in milk with medium somatic cell counts, high somatic cell counts and clinical mastitis comparated with low somatic cell counts. An abundant caseins (α-, β- and κ-caseins) were degraded, while low abundant protein serum albumin, transferrin, fibrinogen beta chain, and haptoglobin could be identified in clinical mastitic milk.【Conclusion】Results suggested that caseins were degraded in milk with medium somatic cell counts, and significantly increased in high somatic cell counts, and abruptly increased in clinical mastitis. The findings may provide a survey of milk protein modulation and increase the knowledge of proteomic profile of milk protein changes with low somatic cell counts, medium somatic cell counts, high somatic cell counts and clinical mastitis to improve the technological quality of milk.

    Isolation of Differentially Expressed Genes between Lantang Pig and Large White Pig by SSH and Q-PCR

    CAI Geng-yuan;; CHEN Yao-sheng;; LI Jia-qi; ZHANG Hao; WANG Chong
    Scientia Agricultura Sinica. 2011, 44(12):  2553-2560 .  doi:10.3864/j.issn.0578-1752.2011.12.018
    Abstract ( 984 )   PDF (569KB) ( 502 )   Save
    Related Articles | Metrics

    【Objective】The aim of this experiment is to investigate the genes related to meat quality, and study its genetic basis and molecular mechanism.【Method】A subtracted cDNA library was constructed by using suppression subtractive hybridization, differential expressions of the genes were studied by using real-time quantitative PCR. 【Result】A total of 61 positive clones were screened. A total of 47 effective sequences were found by sequencing in the whole subtractive cDNA library. After comparisons with GenBank, GenBank ESTs librarys using the online software of the BLAST, the two unknown sequences were found. The expressions of the MYL1, LDHA, KPNA3, TPM3, HUMMLC2B, GNTN, and Oaz genes were studied by real-time quantitative PCR. The results showed that significant differences were found between the Lantang Pig and Large White Pig. The expressions of the MYL1, LDHA, KPNA3, TPM3, and HUMMLC2B genes were significantly higher in the Lantang Pig (P<0.05), and the expressions of the GNTN and Oaz genes were significantly higher in the Large White Pig (P<0.05). 【Conclusion】Many of these ESTs were highly homologous with the important genes related to meat quality traits, and significant differences of them were found between Lantang Pig and Large White Pig.

    Filtration of Transgenic Sheep Skin Fibroblasts with KAP6.1-GFP-polymerized Spider Dragline Silk Protein Gene(4S)
    WANG Chun-sheng; YUAN Lu; NING Fang-yong; WU Zhi-hao; PIAO Shan-hua; AN Tie-zhu
    Scientia Agricultura Sinica. 2011, 44(12):  2561-2566 .  doi:10.3864/j.issn.0578-1752.2011.12.019
    Abstract ( 803 )   PDF (356KB) ( 558 )   Save
    Related Articles | Metrics

    【Objective】This study aims to establish transgenic sheep fibroblast cell line and lay a foundation for transgenic sheep with expression spider dragline silk protein gene in hair follicle by nuclear transplantation.【Method】pcDNA3.1 and pGM-T-KAP6.1(hair follicle-specific promoter) were digested by BglⅡand Hind Ⅲ,and linked each other. The recombinant plasmid was linked with spider dragline silk protein gene and then linked with pIRES2-EGFP by a series of molecular methods. The eukaryotic expression vector pIRES2-EGFP-4S was constructed. Sheep fibroblasts were transfected with the plasmid by cationic liposome method and G418 was used to filtrate them. After identified, transgenic cell line with spider dragline silk protein gene was established. 【Result】The G418 positive cells were detected in vitro. The results showed that cellular morphology was similar with normal fibroblast, cell growth curve was “S” shape, population doubling time (PDT) shorten gradually along with culture time increase, plating efficiency was gradually increasing in 24h. These biological analyses showed the transgenic cells were similar with normal sheep fibroblast. Biological characters of the transgenic cells after freezing-thawing were similar with fresh sheep fibroblast. The PCR result showed that the vector constructed was integrated into sheep genome. 【Conclusion】The transgenic cells with polymerized spider dragline silk protein gene were filtrated, and thus laid a foundation for the transgenic sheep with expression spider dragline silk protein in hair follicle.

    The Genetic Effects of 5′ Flanking Region of GHRL Gene on Chicken Growth and Carcass Traits
    FANG Mei-xia ; XU Hai-ping ; XIE Liang ; LIANG Wei-tao ; LAI Zhe-ying ; ZHANG De-xiang ; NIE Qing-hua ; ZHANG Xi-quan
    Scientia Agricultura Sinica. 2011, 44(12):  2567-2574 .  doi:10.3864/j.issn.0578-1752.2011.12.020
    Abstract ( 747 )   PDF (538KB) ( 478 )   Save
    Related Articles | Metrics

    【Objective】Ghrelin (GHRL) gene plays an important role in chicken growth and development and it is a candidate of great interest for growth and carcass traits. In this study, association analyses were carried out between polymorphisms of the GHRL gene and chicken growth and carcass traits.【Method】Six SNPs of the GHRL gene were genotyped in a F2 full sib chicken population and two commercial populations to investigate their associations with production traits, mRNA expression in glandular stomach and plasma GHRL level. Then the interaction between A-2220C in 5′ flanking region of the chicken GHRL gene and nuclear proteins was analyzed to confirm the effect of this DNA fragment on gene transcription by electrophoretic mobility shift assay (EMSA) method.【Result】Marker-trait linkage study showed that A-2220C was significantly associated with birth weight (P<0.05). Moreover, A allele was positive for chicken birth weight as individuals with AA genotype had the highest value. C-2399Del was significantly associated with body weight (BW) at 56 days, daily gain from weeks 0 to 4, breast muscle weight (BMW), as well as wing weight (WW) (P<0.05). Meanwhile, individuals with
    CDel genotype had the highest value of these traits. On the other hand, haplotypes composed of these 6 polymorphisms were significantly associated with body weight at 42(BW42) and 70(BW70) days of age daily gain from weeks 0 to 4, daily gain from weeks 4 to 8, dressed weight, eviscerated weight, eviscerated weight with giblet, and BMW (P<0.05), and they were also significantly associated with birth weight and body weight at 21(BW21), 28(BW28), 35(BW35), 49(BW49), 56(BW56) and 90(BW90) days of age (P<0.01). Furthermore, individuals with haplotype H3H4(CCAAAC\CCAACDel)was positive for chicken growth compared to individuals with other haplotype combinations. The results of EMSA showed that fragment with A allele of A-2220C could bind the nucleic protein, whereas that with C allele was free of binding activity. And it suggested that this DNA fragment has some effect on the GHRL gene transcription.【Conclusion】The polymorphisms of the 5′ flanking region of GHRL gene are significant association with chicken growth and carcass traits.

    Analysis of Bacterial Community and Screening and Identification of Enzyme-Producing Bacteria in Intestine of Antheraea pernyi
    ZOU Chang-rui; WEI Guo-qing; LIU Chao-liang; ZHU Bao-jian; WANG Zai-gui; YANG Wen-jing
    Scientia Agricultura Sinica. 2011, 44(12):  2575-2581 .  doi:10.3864/j.issn.0578-1752.2011.12.021
    Abstract ( 919 )   PDF (463KB) ( 722 )   Save
    Related Articles | Metrics

    【Objective】The objective of this study is to isolate and identify bacterial community and enzyme-producing bacteria in intestine of Antheraea pernyi larvae and to develop microecological agents for increasing leaf-silk conversation rate and disease resistance. 【Method】Bacteria were isolated from intestine of fifth instars Antheraea pernyi larvae reared on oak leaves by isolated culture. Intestinal bacterial community was identified according to physiological and biochemical characteristics and phylogenetic analysis based on 16S rDNA sequences. Cellulase, protease, lipase-producing strains were screened on selective medium. 【Result】The intestinal bacteria isolated from Antheraea pernyi larvae belong to Bacillus, Staphylococcus and Enterobacter. Among them, Bacillus is the main bacteria and the main enzyme-producing bacteria which could produce cellulase and protease, Staphylococcus could produce protease weakly, Enterobacter couldn’t produce enzyme.【Conclusion】Intestinal bacteria community of Antheraea pernyi was similar to that of Bombyx mori, which could be developed as microecological agents in sericulture for the enzyme-producing strains exhibiting high activity.

    VETERINARY SCIENCE
    Construction and Immunogenicity of DNA Vaccines Containing GP5 Gene Against PRRSV with Porcine C3d as Molecular Adjuvant
    XIA Qing-xiang; ZHANG De-qing;;; WU Jia-qiang; NIU Xing; WANG Xiao-long; NIU Zhong-xiang
    Scientia Agricultura Sinica. 2011, 44(12):  2582-2588 .  doi:10.3864/j.issn.0578-1752.2011.12.022
    Abstract ( 739 )   PDF (538KB) ( 527 )   Save
    Related Articles | Metrics

    【Objective】DNA vaccines containing PRRSV GP5 gene with porcine C3d-p28 were constructed and the enhancing effect of molecular adjuvant C3d-p28 was proved. 【Method】Pigs were injected with the oil emulsion of Escherichia coli inactivated vaccine in order to induce immune activation. After cloning the C3d cDNA of pig by using liver as the source of mRNA, a pair of primers was designed to sub clone the P28 gene to the pUC19 plasmid. Several tandems of p28 were constructed in the pUC19 plasmid using a pair of isoschizomers BamHI and Bgl II. The genes of p28.n, which were digested from pUC19-p28.n, were cloned into pcDNA3.1 (+) plasmid. The GP5 gene of PRRSV was cloned by RT-PCR and inserted into the upstream of pcDNA3.1-p28.n plasmid, and the DNA vaccines containing GP5 gene with C3d-p28 as molecular adjuvant were successfully constructed. All recombinant plasmids were verified by PCR, restriction endonuclease digestion and DNA sequencing. In addition, indirect immunofluorescence assay (IFA) could be found within the cells after Mark145 cells tranfected by recombinant plasmids. Furthermore, seven groups of mice were injected with these recombinant plasmids. GP5-specific ELISA antibody, IFN-γlevel and IL-4 level in the sera were detected. 【Result】It was showed that the C3d cDNA of pig was cloned and several tandems of P28 and pcDNA3.1 (+)-GP5-C3d-p28.n were successfully constructed. Serological analysis showed that BALB/c mice that vaccinated with DNA vaccine expressing fusions of pcDNA3.1-GP5-p28 (two, four or six repeats) elicited a higher level of humoral response compared with mice that vaccinated with DNA vaccine expressing only the pcDNA3.1 vector and pcDNA3.1-GP5 group (P <0.05). The increase in the immune response elicited by six copies of p28 was the highest. 【Conclusion】The DNA vaccine containing PRRSV GP5 gene with porcine C3d-p28 has been successfully constructed, which could elicit humoral and cellular immune responses. The complement C3d–p28 can significantly enhance the nucleic acid vaccine immune effect as molecular adjuvant.

    Study on the Cloning and Tissue Expression of Neuropeptide S and Its Receptor and Effect of Heat Stress on Their Expression in the Rabbit

    HOU Yu-jing; LEI Zhi-hai; SU Juan; YAO Yuan; LI Yue-qin; YU Xiao-lei; LIU Qian
    Scientia Agricultura Sinica. 2011, 44(12):  2589-2600 .  doi:10.3864/j.issn.0578-1752.2011.12.023
    Abstract ( 735 )   PDF (1068KB) ( 433 )   Save
    Related Articles | Metrics

    【Objective】This study was carried out to obtain sequence of NPS and NPSR gene, and reveal the expression of NPS and NPSR mRNA in rabbit organs and influence of heat stress on expression of NPS/NPSR mRNA in the hypothalamus- pituitary- adrenal(HPA) axis. 【Method】The complete coding sequence (CDS) of NPS and partial CDS of NPSR were obtained from rabbit brain using the approach of gene cloning. The expression of NPS and NPSR mRNA in rabbit organs and influence of heat stress on expression of NPS/NPSR mRNA in the hypothalamus-pituitary-adrenal axis were studied by semi-quantitative RT-PCR. 【Result】The length of NPS and NPSR were 270 bp and 847 bp, respectively. The sequences of the products were confirmed to be those of NPS and NPSR in rabbit and enrolled in GenBank(accession numbers:EU978456 and FJ713102). Amino sequece of rabbit NPS precursor had high homology compared with other animals. Phylogenetic analysis showed that amino sequence of rabbit NPS precursor had high homology compared with rat and mouse consistent with species evolution relationship. The expression of NPS and NPSR mRNA was extensive in rabbit organs, with majority in the central nervous system, thyroid gland, salivary gland and mammary gland. After the heat stress NPS mRNA expression level in the hypothalamus and the adrenal gland increased gradually and followed by a downward trend afterwards, that is, it reached the highest at 1 h and 0.5 h compared with the control and showed a significant difference (P<0.01), and followed by decline at 2 h. In the pituitary the
    experimental group showed a downward trend and showed a significant difference compared with the control (P<0.01). NPSR mRNA expression patterns in the hypothalamus- pituitary-adrenal axis was similar to that of NPS during heat stress compared with the control. 【Conclusion】NPS and its receptor gene in rabbit were conservative, the expression of NPS and NPSR mRNA in rabbit organs were extensive and the expression of NPS and NPSR mRNA in HPA changed when rabbit was suffered from heat stress.

    RESEARCH NOTES

    Induction of NO and the Relationship Between NO And Ca2+ in Wheat Suspension Cells Induced by IWF

    CHEN Yang-hui; LIU Jing; CHEN Yan; HOU Chun-yan; WANG Dong-mei
    Scientia Agricultura Sinica. 2011, 44(12):  2601-2607 .  doi:10.3864/j.issn.0578-1752.2011.12.024
    Abstract ( 738 )   PDF (363KB) ( 540 )   Save
    Related Articles | Metrics

    【Objective】Intercellular washing fluid (IWF) obtained from wheat leaves infected by leaf rust contains specific elicitors that was used to stimulate the suspension cell of wheat (cv. Lovrin 10) in order to study the nitric oxide (NO) acting and its possible molecular mechanism.【Method】Greiss reagent and fluorescent NO probe DAF-2DA were used to detecting of dynamic changes of intracellular NO. Pharmacological experiments were carried out to study the signal interactions between calcium (Ca2+) and NO.【Result】IWF-260 could cause the production of NO in wheat suspension cultured cells. It played an important role in the programmed death of wheat suspension cells which was induced by IWF-260. Pharmacological inhibitor experiments proved that NOS and NR might be involved in the production of NO, and the influx of extracellular Ca2 + could regulate the production of NO. NR pathway may be the major route in NO production. 【Conclusion】IWF-260 can induce the production of NO in wheat suspension cells, and this process is involved with the NOS, NR and the influx of extracellular Ca2+.

    Study on the Effect of Maize Plants on Rainfall Redistribution Processes
    LIN Dai-jie; ZHENG Zi-cheng;; ZHANG Xi-zhou; LI Ting-xuan; WANG Yong-dong
    Scientia Agricultura Sinica. 2011, 44(12):  2608-2615 .  doi:10.3864/j.issn.0578-1752.2011.12.025
    Abstract ( 650 )   PDF (360KB) ( 795 )   Save
    Related Articles | Metrics

    ObjectiveThe purpose of this study was to investigate the rainfall redistribution processes through maize canopy.MethodRainfall simulator was used to measure mature maize canopy interception, throughfall and stemflow based on the actual rainfall intensity.ResultThe averages of throughfall, stemflow and canopy interception account for 7.5%, 28.8% and 63.7% of the total precipitation, respectively. Canopy interception, stemflow and throughfall were statistically significantly positive correlated with rainfall intensity, and canopy interception and stemflow were statistically significantly positive correlated with leaf area, while throughfall was statistically significantly negative relationship with leaf area.ConclusionUnder different rainfall intensities, canopy interception capacity was different, canopy and stemflow can guide a part of total rainfall, reduce the amount of rainfall arrived on the soil and influence runoff. Compact and practical equations built in this study has provided a theoretical basis for farm land soil erosion control.

    Osmotic Adjustment of I. lactea var.chinensis Leaves Under Interactions of Soil Moisture and Salinity

    ZHANG Biao; LI Pin-fang; BAI Hai-feng; FAN Fen-cheng
    Scientia Agricultura Sinica. 2011, 44(12):  2616-2624 .  doi:10.3864/j.issn.0578-1752.2011.12.026
    Abstract ( 926 )   PDF (409KB) ( 663 )   Save
    Related Articles | Metrics

    【Objective】The objective of this study was to investigate the growth changes and osmotic adjustment of perennial herbaceous Iris plant (Iris lactea Pall. var. chinensis (Fisch.)Koidz.)under interactions of soil moisture and salinity, and to provide a scientific basis for cultivation and utilization of I. lactea var. chinensis.【Method】I. lactea var. chinensis seedlings under sand culture condition were treated with the 15 cross combinations of different NaCl concentrations of 0(CK), 0.5, 1, 1.5, and 2% and three soil moisture levels of 100, 65, and 45%(of field capacity). After 8 d treatment, the contents of inorganic ions, proline (Pro) and soluble sugar (SS) in shoots were determined, respectively, and their contributions to the osmotic potential were calculated.【Result】The optimal growth condition for I. lactea var. chinensis was high soil moisture and low salinity, 1.5%-2% NaCl caused a significant decrease in shoot biomass, while decrease in soil moisture from 100% of field capacity to 65% resulted in a 14% increase in shoot biomass at 2% NaCl. At 45% of field capacity, biomass was not significantly different between 0.5% and 1% NaCl. Contents of Na+, Cl- and Pro in shoots significantly increased with the decrease of moisture levels of soil. More inorganic ions played an important role in osmotic adjustment with soil moisture decreasing, estimated contribution of inorganic ions to osmotic potential was 90% at 1.5% NaCl combined with 45% of field capacity. It had no significant effect on estimated contribution of Pro and SS to osmotic potential.【Conclusion】The growth of I. lactea var. chinensis at high salinity was increased by moderate moisture level, and the drought tolerance of I. lactea var. chinensis was improved by moderate salinity. As soil moisture decreased, inorganic ions played a more important role in osmotic adjustment, more inorganic ions but less organic osmoticum were involved in osmotic adjustment in leaves of I. lactea var. chinensis.

    Inhibition Effect of Garlic Root Exudates on the Genus Allium

    LIU Su-hui; LIU Shi-qi; ZHANG Zi-kun; WEI Hui;; HUANG Zhi-jun; ZHANG Yu
    Scientia Agricultura Sinica. 2011, 44(12):  2625-2632 .  doi:10.3864/j.issn.0578-1752.2011.12.027
    Abstract ( 1115 )   PDF (465KB) ( 776 )   Save
    Related Articles | Metrics

    【Objective】In order to provide a theoretical basis for solving cropping obstacles of garlic, the inhibition of garlic root exudates on the genus Allium was studied. 【Method】In order to avoid interferences from external factors, this paper studied the autotoxicity of garlic root exudates on welsh onion, onion and Chinese leek scallion, by using tissue culture under sterilized condition. 【Result】Garlic and receptor crops, such as welsh onion, onion and Chinese leek, are belong to the genus Allium. Root exudates of garlic could inhibit the germination and growth of receptor crops, the inhibitory degree increased progressively and significantly with increasing root exudates concentrations of garlic, and the maximal inhibition rates of germination rate, mean time to germination, embryo and radicle length, embryo and embryo fresh weight were 51.72%, 48.20%, 51.57%, 51.57%, 82.14% and 82.29%, respectively. Garlic root exudates decreased root activity of receptor crops, and significant differences were observed except the lowest concentrations (T1) on welsh onion and onion, compared to control. Compounds involved in the garlic root exudates were isolated and identified by gas chromatography/mass spectrometer (GC-MS), including 2,2′-methylenebis[6-(1,1-dimethylethyl)- 4-methyl-Phenol, mono (2-ethylhexyl) phthalate, eicosanoic acid, ferulic acid, 9-hexadecenoic acid, tetradecanoic acid, diallyl disulphide, etc., ferulic acid and myristic acid which had been identified as growth inhibitory substances.【Conclusion】The germination and growth of receptor crops were inhibited by garlic root exudates, which were released to field year by year and could lead to garlic continuous cropping obstacles.