Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (13): 2830-2837 .doi: 10.3864/j.issn.0578-1752.2011.13.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Bacteriostatic Activity of Bacteriocin Subtilin112 Against Staphylococcus aureus CVCC 1885 in Vitro and in Vivo

DENG kai;SU Li-fang;ZHANG Ri-jun   

  1. 中国农业大学动物科技学院饲料生物技术实验室/动物营养学国家重点实验室
  • Received:2010-12-07 Revised:2011-03-11 Online:2011-07-01 Published:2011-07-01
  • Contact: Zhang Rijun

Abstract: 【Objective】 In order to provide the theoretical basis for clinical application of the bacteriocin Subticin112 secreted by Bacillus subtilis, a research was conducted on bacteriostatic activity of bacteriocin Subticin112 against Staphylococcus aureus CVCC 1885 in vitro and in vivo. 【Method】 Bacteriostasis in vitro: Subticin112, oxytetracycline, penicillin, vancomycin, neomycin, strepomycin and gentamicin in the same effective weight were dissolved into equal volume of sterile saline, respectively. Then the agar diffusion method was used to compare the bacteriostatic activity of the medicines against S. aureus. Bacteriostasis in vivo: 120 SPF-class KM male mice (weighted from 18 g to 22 g) were divided into six groups, they were blank control group, negative control group, oxytetracycline treating group and three Subticin112 treating groups. After the beginning of the experiment, every mouse in each group was orallly administrated 0.4 mL different concentrations of medicine or sterile saline every other 8 hours, after 3 times of oral administration, the mice in each group were intraperitoneal injected at the absolute lethal dose of S. aureus or sterile saline. At the end of the experiment, the mortality was calculated, the proinflammatory cytokines and the cfu values of S. aureus in the peritoneum of the mice were determined, and then the histopathological changes of the tissues were observed. 【Result】Bacteriostasis in vitro: Subticin112 expressed a fine bacteriostatic activity compared to the same concentration of other medicines. The bacteriostatic activity of Subticin112 was highly significantly better than that of vancomycin and neomycin (P<0.01), but differed insignificantly from other antibiotics (P>0.05). Bacteriostasis in vivo: after the experiment, the mortality in negative control group was 100%, while which was 30% in low-dose Subticin112 treating group, and other groups did not have dead mice. The medicine treating groups showed a low concentration of the proinflammatory cytokines (P<0.05), also had a low cfu values compared to the negative control group (P<0.01) and the dose-respones relationship was clear. The histopathological observations showed that the liver and spleen of the mice in the negative control group had obvious pathological lesions, whereas the pathological changes in the medicine treating groups was slight or not distinct. 【Conclusion】These studies provided evidences that Subticin112 have a fine bacteriostatic activity against S. aureus CVCC 1885 in vitro and vivo, and can be developed as a new type of antibacterial agent.

Key words: Bacillus subtilis bacteriocin, Staphylococcus aureus, bacteriostatic activity, absolute lethal dose

[1] TONG Yong-Juan-1, SA Ren-Na-1, ZHANG Hong-Fu-1, SUN Chun-Yang-1, XING Huan-1, PENG Yi-Zhu-1, WEI Zhong-Hua-2, WANG Xue-Jing-2. Comparative Studies on the Bacteriostatic Activity of the Three Bacillus Strains and Its Effects on Air Microorganisms in Broiler House [J]. Scientia Agricultura Sinica, 2013, 46(20): 4344-4353.
[2] YANG Jing, YANG Jun, HUANG Ji-Chao, HE Wei-Ling, ZHANG Chi, HUANG Ming. Distribution and Temporal Expression of Staphylococcus aureus Enterotoxin Genes in Foodborne Staphylococcus aureus Strains [J]. Scientia Agricultura Sinica, 2012, 45(19): 4057-4066.
[3] SU Yang-., PU Wan-Xia, CHEN Zhi-Hua, DENG Hai-Ping. Antimicrobial Resistance Analysis and Detection of Methicillin- Resistant Staphylococcus aureus (MRSA) Among Staphylococcus aureus Strains Isolated from Bovine Mastitis [J]. Scientia Agricultura Sinica, 2012, 45(17): 3602-3607.
[4] NI Chun-xia,PU Wan-xia,HU Yong-hao,DENG Hai-ping
. Research on Coagulase Genotyping of Staphylococcus Aureus Isolated from Bovine Mastitis
[J]. Scientia Agricultura Sinica, 2011, 44(2): 417-422 .
[5] XU Yi-gang,LI Su-long,LI Dan-dan,JIANG Yan-chun,XIE Xiao-feng
. Establishment of Rapid Method for Detecting Staphylococcus aureus in Food by Loop-Mediated Isothermal Amplification #br# [J]. Scientia Agricultura Sinica, 2010, 43(8): 1655-1663 .
[6] YANG Yu-hui,LI Xiao-chun,HAN Xin-chou,WANG Xue-mei,WU Ke-bang,HU Ri-cha
. Study on Pharmacokinetic/Pharmacodynamic Model of Amoxicillin Against Staphylococcus aureus in Serum and Tissue Fluid of Pigs ex vivo#br# [J]. Scientia Agricultura Sinica, 2010, 43(8): 1723-1729 .
[7] HE Na,WANG Chang-fa,YANG Hong-jun,HE Hong-bin,YANG Shao-hua,WANG Li-qun,GAO Yun-dong,ZHONG Ji-feng
. Screening an Attenuated Strain and Immunogenicity in Mice of a Bovine Mastitis Staphylococcus Aureus Mutant
[J]. Scientia Agricultura Sinica, 2010, 43(10): 2174-2181 .
[8] . Expression of β-hemolysin Gene of Staphylococcus aureus Strains Shandong (zfb) and Its Hemolytic Activity
[J]. Scientia Agricultura Sinica, 2009, 42(1): 324-330 .
[9] ,,,,. Detection of Staphylococcus aureus in Dairy Products by Polymerase Chain Reaction Assay [J]. Scientia Agricultura Sinica, 2006, 39(05): 990-996 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] ,. A Review on Reproduction Biology in Diospyros kaki L.[J]. Scientia Agricultura Sinica, 2006, 39(12): 2557 -2562 .
[2] . Ecological, Physiological and Molecular Mechanisms of Enhanced Nitrate Nutrition in Rice[J]. Scientia Agricultura Sinica, 2008, 41(6): 1708 -1716 .
[3] . Effects of insect-resistant cry1Ac/sck transgenic rice on the key non-target pests in paddy fields and evaluating the ecological safety[J]. Scientia Agricultura Sinica, 2007, 40(6): 1181 -1189 .
[4] . Study on Browning Mechanism of Pomegranate Peel during Storage and Technology of Preventing Pomegranate Brown[J]. Scientia Agricultura Sinica, 2007, 40(3): 573 -581 .
[5] ,. [J]. Scientia Agricultura Sinica, 2005, 38(04): 678 -683 .
[6] ,. Effect of Nitrogen Application Rate on Noodle-making Quality of Wheat[J]. Scientia Agricultura Sinica, 2005, 38(02): 420 -424 .
[7] YANG Yan-li,XIAO Lang-tao,HU Xian-qi. Study on the Relationship Between the Toxin of Phytophthora Infestans and Resistance of Potato
[J]. Scientia Agricultura Sinica, 2009, 42(6): 2202 -2210 .
[8] . Effect of Irrigation Method on Yield and Quality of Flue-cured Tobacco Under Different Fertilization Levels
[J]. Scientia Agricultura Sinica, 2009, 42(1): 173 -179 .
[9] . [J]. Scientia Agricultura Sinica, 2008, (12): 3999 -4007 .
[10]

. [J]. Scientia Agricultura Sinica, 2008, 41(10): 3240 -3248 .