Abstract:

【Objective】 The study is to develop diversified DH lines in pakchoi for the practice of heterosis breeding. 【Method】 Isolated microspore culture was employed in 15 hybrid varieties. An efficient culture system was established through the optimization of culture conditions. 【Result】 Totally 373 regenerated plants were obtained, in which 251 were identified as double haploid. Furthermore, seven excellent DH lines were screened out. Two improved F1 combinations were made based on such DH lines. 【Conclusion】 A technical system was successfully established for heterosis breeding of pakchoi with DH lines as parents created by isolated microspore culture.

Key words: pakchoi')">pakchoi, isolated microspore culture, DH line, heterosis breeding