Abstract:

【Objective】 Chinese cabbage-cabbage alien addition line was screened, alien chromosomes introduced into Chinese cabbage were identified by SSR, and the characters of the alien addition line were further analyzed. 【Method】 The BC1F2 progenies derived from allotriploid hybrid (AAC) and diploid Chinese cabbage (AA) were used as materials, by using analysis of the root tip cell chromosome number and linkage group-specific SSR markers from linkage groups of cabbage, the Chinese cabbage-cabbage alien addition line was selected and identified. 【Result】 A plant (2n=22) was obtained. Amplification results of 3 pairs of primers assigned linkage group 01 and 2 pairs of primers assigned 08 linkage group on this plant were the same as allotriploid hybrid, however, the amplification results of 20 pairs of primers assigned other 7 linkage groups were the same as Chinese cabbage. Therefore, the introduced cabbage chromosomes in this plant were grouped into linkage groups 01 and 08 of cabbage, which was double alien monosomic addition line. Ten bivalents and two univalents were observed at the stage of diakinesis in most of double alien monosomic addition line’s PMCs, 11-11 and 10-12 distributions were observed at anaphaseⅠ in most of PMCs, and the gametes with n=11 were observed at anaphaseⅡ. 【Conclusion】 Chinese cabbage-cabbage addition alien line could be exactly identified by specific SSR markers for cabbage compared with Chinese cabbage. And Chinese cabbage-cabbage double alien monosomic addition line added linkage groups 01 and 08 of cabbage was obtained, which laid a foundation for further obtaining the Chinese cabbage-cabbage monosomic alien addition lines and disomic alien addition lines added linkage group 01 or 08 of cabbage.

Key words: Chinese cabbage, cabbage, alien addition line, linkage group-specific SSR, character