Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (5): 1639-1645 .doi: 10.3864/j.issn.0578-1752.2009.05.017

• PLANT PROTECTION • Previous Articles     Next Articles

Gene Cloning and Tissue-Specific Expression of an Olfactory Receptor in Microplitis Mediator (Hymenoptera: Braconidae)

  

  1. 中国农业科学研究院植物保护研究所/植物病虫害生物学国家重点实验室

  • Received:2008-05-29 Revised:2008-07-07 Online:2009-05-10 Published:2009-05-10
  • Contact: GUO Yu-yuan

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Abstract:

【Objective】 The expression of an olfactory receptor of Microplitis mediator was studied. 【Method】 The olfactory receptor gene of Microplitis mediator was cloned using RT-PCR. And its tissue-specific expression and quantitative changes at different developmental stages were detected using real-time PCR. 【Result】 A gene encoding a novel olfactory receptor, which is antenna- specific, was isolated from Microplitis mediator (Hymenoptera: Braconidae). Its expression levels reached the maximum amount on the emergence day of female and reached the maximum amount at the d stage before emergence of male. 【Conclusion】This receptor is antenna- specific in Microplitis mediator.

Key words: Microplitis mediator, olfaction, olfactory receptor, expression pattern

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