Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (5): 1616-1623 .doi: 10.3864/j.issn.0578-1752.2009.05.014

• PLANT PROTECTION • Previous Articles     Next Articles

Proteomic Analysis of Stripe Rust Resistance Wheat Line Taichung29*6/Yr5 Inoculated with Stripe Rust Race CY32

  

  1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良国家重大科学工程
  • Received:2008-06-05 Revised:2008-07-11 Online:2009-05-10 Published:2009-05-10
  • Contact: PAN Ying-hong

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Abstract:

【Objective】 To investigate the biochemical changes of wheat resistance to stripe rust after infected with the stripe rust, a resistive line of wheat (Taichung29*6/Yr5) infected with stripe rust CY32 was compared to mock-inoculated wheat by comparative proteomics technology. 【Method】 Proteins were extracted from leaves infected with a virulent race of stripe rust CY32 and control group after 48 hours of inoculation, and then separated by using two-dimensional electrophoresis (2-DE). The differential protein spots were selected for MALDI-TOF MS analysis and database searching. 【Result】 By using 2-DE，2 uniquely expressed spots, 5 up-regulated spots and 6 down-regulated spots were observed in Taichung29*6/Yr5 leaves inoculated with stripe rust CY32. Total of 11 proteins, including pathogenesis-related homeodomain protein, beta-glucosidase, glutathione transferase etc, were identified. 【Conclusion】 Through analysis of protein function , it is presumed that these differential proteins are correlated with the infextation of stripe rust race CY32.

Key words: proteomics, wheat, stripe rust resistance, two-dimensional gel electrophoresis, PMF

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