Abstract:

【Objective】 To study the serum pharmacochemistry of Andrographis paniculata in chickens firstly. 【Method】Based on the established HPLC-MS fingerprints of Andrographis paniculata ultra- fine powder,analysis and comparison were carried out among the HPLC-MS profiles of chicken serum samples obtained after oral administration and control serum, and then the compounds absorbed into blood, their original crude drugs and their metabolites were identified. 【Result】 Nine components were found in chicken serum after oral administration at 60 min, the four compounds of them ,the main ingredients, were originated from Andrographis paniculata and their higher level of serum consentrations maintained from 0-480 min; other compounds should be metabolites. 【Conclusion】 The compounds are absorbed into blood and their metabolites would be the effective constituents and their serum pharmacochemistry should be subject to complete investigation so as to lay a foundation for the further study of active ingredients of Andrographis paniculata.

Key words: Andrographis paniculata')">Andrographis paniculata, ultra-fine powder, HPLC-MS, chicken, serum pharmachemistry