Abstract: Three key enzymes AGPP, SSS and SBE associated with starch synthesis were studied during grain filling of three winter wheat cultivars with high，medium and low gluten content in the clay，loam and sandy soil texture in the condition of pond culture. The results indicated that three enzymes activities of high gluten content cultivars were higher in the sandy soil，AGPP activity came to be a double-peak curve，its peaks appeared at 10 d and 20 d after anthesis，respectively. SSS and SBE activities had a single-peak curve，its peak appeared at 20 d after anthesis. It showed that sandy soil could be helpeful to high gluten content cultivars in synthetise starch. AGPP and SSS activities of medium gluten content cultivars showed "sand＞loam＞clay"，but SBE activities of clay and loam soil were higher than that of sandy soil. It showed that three kinds of soil texture could be helpeful medium gluten content cultivars to synthesize starch. Three enzymes activities of low gluten content cultivars were higher in the clay soil，its changing trend came to be a single-peak curve，the peak appeared in 20 d after anthesis. It showed that clay soil could be helpeful low to gluten content cultivars to synthesize starch.

Key words: Winter wheat, Soil texture, Gluten content, Starch, Synthesis enzyme