Abstract: 【Objective】Aim of this research is to clone and analyses the gp41 gene of Clanis bilineata Nuclear Polyhedrosis Virus (CbNPV), which will be helpful to obtain the molecular information of this virus.【Method】CbNPV was purified from Clanis bilineata larva, the genomic DNA was extracted, and a DNA fragment library of the virus was constructed by shotgun. The positive clones were sequenced and we analyzed the open reading frame which has high identity with the gp41 gene of most baculovirus. 【Result】The gp41 gene of CbNPV is 933 bp long and encodes a protein of 310 amino acids. The amino acid sequence analysis indicated that the CbNPV GP41 has 53%-61% and 56%-73% identities with Group I and II NPVs GP41 proteins, respectively. 【Conclusion】The result indicates that the CbNPV isolated by the authors is a novel baculovirus and the CbNPV has more closer relationship with Group II NPVs.

Key words: Clanis bilineata Nuclear Polyhedrosis Virus, gp41 gene, Sequence analysis