Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1706-1715.doi: 10.3864/j.issn.0578-1752.2013.08.021

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning and Expression Profiles Analysis of Chemosensory Protein Genes Family in the Chinese Honeybee (Apis cerana cerana)

 NI  Cui-Xia, ZHANG  Lin-Ya, LI  Hong-Liang, SHANG  Han-Wu   

  1. College of Life Sciences, China Jiliang University/Key Laboratory of Biometrology and Inspection& Quarantine of Zhejiang Province, Hangzhou 310018
  • Received:2012-10-19 Online:2013-04-15 Published:2012-11-19

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Abstract: 【Objective】 The objective of this study is to clone the whole genes family of chemosensory protein in the Chinese honeybee (Apis cerana cerana) (Ac-CSPs) and analyze their expression of mRNA in different tissues and different development time-points. This may provide a fundamental evidence for the future study of the physiological function of Ac-CSPs. 【Method】 Six full-length of CSP genes of A. cerana cerana was cloned by using reverse transcription-polymerase chain reaction (RT-PCR). The expression patterns at development phases and in different sensory organs of the six Ac-CSPs were analyzed by real-time PCR. 【Result】 Four full-length of Ac-CSP genes (Ac-CSP2, Ac-CSP4, Ac-CSP5 and Ac-CSP6) were cloned in which Ac-CSP2 was 354 bp in full length that encoded a polypeptide containing 117 amino acids with prediction of MW (molecular weight) of 13.01 kD and pI of 8.86, respectively. Ac-CSP4 was 387 bp in full length that encoded a polypeptide containing 128 amino acids with prediction of MW of 14.61 kD and pI of 4.53, respectively. Ac-CSP5 was 315 bp in full length that encoded a polypeptide containing 104 amino acids with prediction of MW of 12.46 kD and pI of 9.60, respectively. Ac-CSP6 was 378 bp in full length that encoded a polypeptide containing 125 amino acids with prediction of MW of 14.63 kD and pI of 8.71, respectively, and all of the genes with four conserved cysteine residues. The six deduced Ac-CSPs sequences showed high sequence similarity (95%-99%) as compared with CSPs (Am-CSPs) sequences of A. mellifera. The genes of Ac-CSP1, 2, 3, 4 were not expressed in eggs, larvae and pupae, with the Ac-CSP1, 2, 4 expressed at highest level in antennae and Ac-CSP3 was strongly expressed in wings. The expression of Ac-CSP5 was not significantly different at all developmental stages, while Ac-CSP6 only highly expressed in the pupae. 【Conclusion】 The alignment is very similar of Ac-CSPs and Am-CSPs and the expression patterns are similar but there is a part of difference in this research, which provides useful information for the study of function of CSPs in the future.

Key words: Apis cerana cerana , chemosensory proteins , molecular cloning , DNA sequence analysis , global expression

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