Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (8): 1587-1593 .doi: 10.3864/j.issn.0578-1752.at-2006-7767

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Foundation, Mapping and Application of a New Repeated DNA Sequence Similar to Sukkula For Rye Genome

  

  1. 电子科技大学生命科学与技术学院
  • Received:2006-06-29 Revised:2006-09-04 Online:2007-08-10 Published:2007-08-10

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Abstract: 【Objective】 In order to find out new repeated sequence as special molecular marker of genome of rye.【Method】A high copies specific DNA segment OPD15940（be cloned and sequenced）of rye genome was obtained by using random amplified polymorphic DNA (RAPD) analysis. A pair of special primers was designed based on the sequence of OPD15940, validated the particularity of OPD15940 by using specific PCR with correlative species. Mapping the segment on chromosomes by using in situ hybridization【Result】pScD15940 was a new repeated DNA sequence similar to Sukkula. The result of in situ hybridization suggested that pScD15940 was specifically hybridized throughout all rye chromosomes arms except for the terminal regions.【Conclusion】pScD15940 can be used as a genome -specific DNA marker for detection chromosome of Secale in wheat.

Key words: Rye, Repeated DNA sequence, in situ hybridization, Specific PCR

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