Abstract: Genome RNA was extracted from muscle of Landes goose,MSTNgene mRNA was amplified by using RT-PCR.The unique DNA fragment about 1128bp in length was obtained and the PCR product was cloned into pGEM-T vector. The MSTN gene was isolated and sequenced from the positive clones screened.Sequence analysis revealed that it has a length of 1128 nucleotides which encodes a 375 amino peptide. Compared with homologous sequence of human, rat,duck and goose,it displayed a fairly high degree conservation.The homologue of the nucleotide sequence are 94%,94%,99% respectively;the homologue of the amino acid sequence are 98%,97%,98% respectively. Based on the MSTN gene clone, using β-actin as inner control, constructing an optimal semi-quantitative RT-PCR method,the difference of MSTN gene expression at 21 days and 70days of Landes goose by feeding different energe diets was researched ,in the same time we detected IGF-I and GH in serum. The results showed the expressions of MSTN in female landes geese were a little higher than male at 21 days;but the expression of MSTN in male was A>C>B by 70d,to female was C>B>A by 70d. In 21～70 days the expression of MSTN was changed with IGF-I in serum;it was not associated with GH in serum,GH was significant correlations with energy.

Key words: Anser anser, MSTN, Muscle, Semi-quantitative RT-PCR, IGF-I, GH