Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (14): 3121-3131.doi: 10.3864/j.issn.0578-1752.2026.14.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Soil Conditioners and Organic Fertilizer Application on Enzyme Activity and Rice Yield in an Acid Paddy Soil

YE JiaYi1,2(), WU QiFeng3, WANG JiaLin1,2, ZHOU Yan1,2, MA XiaoMin1,2, LIANG ChenFei1,2, QIN Hua1,2, CHEN JunHui1,2()   

  1. 1 College of Environment and Resources, Zhejiang A&F University/Zhejiang Key Laboratory of Soil Remediation and Quality Improvement, Hangzhou 311300
    2 College of Carbon Neutrality, Zhejiang A&F University, Hangzhou 311300
    3 Agroforestry Technology Extension Centre of Lin’an District, Hangzhou, Hangzhou 311300
  • Received:2025-09-04 Accepted:2025-10-16 Online:2026-07-16 Published:2026-07-21
  • Contact: CHEN JunHui

Abstract:

【Objective】The effects of application of soil conditioners and their combination with organic fertilizers at a low addition rate on soil acidity, nutrients, enzyme activity and rice yield following three successive years were investigated in this study, so as to provide a theoretical basis for soil improvement in acid red soil in Southern China.【Method】A field experiment was conducted with eight treatments: a control without fertilization and soil conditioner (CK0), traditional fertilization (CK1), application of mineral soil conditioner (0.75 t·hm-2) with traditional fertilization (M), organic soil conditioner (1.2 t·hm-2) with traditional fertilization (O), and combined application of mineral and organic soil conditioners with traditional fertilization (MO), as well as co-application of M, O and MO with organic fertilizer (4.5 t·hm-2) under traditional fertilization (MF, OF and MOF). The soil conditioners and organic fertilizer were applied once per year. After consecutive application for 3 years, rice yield was measured and soil chemical properties and soil enzyme activity were analyzed.【Result】Compared with CK0 and CK1, all the treatments significantly increased soil pH, respectively, and significantly reduced soil exchangeable H+, exchangeable Al3+, and exchangeable acid concentrations. Compared with CK1, MF, OF, and MOF treatments significantly increased soil organic matter content, while MOF treatment also increased available phosphorus (P) and potassium (K) content, microbial biomass carbon and nitrogen and rice yield. Compared with the CK1, the application of MF and OF significantly reduced the activity of acid phosphatase, while MOF significantly reduced the vector angle, indicating a decreased microbial phosphorus (P) limitation. Soil exchangeable H+, exchangeable Al3+, and exchangeable acid concentrations had positive correlations with the activity of acid phosphatase, while soil organic matter, pH, available N, microbial biomass carbon, available P had negative correlations with it. Redundant analysis indicated that decrease in soil potential acidity and increase in soil nutrients were the key drivers of soil enzyme activities. Correlation analysis suggested that rice yield was significantly positively correlated with soil organic matter, available N, and available P, and significantly negatively correlated with exchangeable acids and the vector angle. Structural equation modeling further indicated that variation in soil organic matter and exchangeable acid changes rice yield via microbial biomass carbon and P limitation, which explained 62% variation in rice yield. Soil organic matter had a significant positive effect on rice yield, while the exchangeable acid concentration and vector angle had negative effects on it.【Conclusion】The combined application of mineral or organic soil conditioners with organic fertilizer following three successive years reduced soil acidity, improved soil organic matter and nutrient availability and mitigated microbial P limitation. The combination of the three amendments had the best improvement on rice yield, thereby can be recognized as an effective way for reducing acidity and improving soil fertility in red soil in the south China.

Key words: soil acidification, organic fertilizer, soil conditioner, rice yield, enzyme activity

Table 1

Addition rate of soil conditioners in each treatment"

编号 Number 处理
Treatment
矿物源调理剂
Mineral conditioner (t·hm-2)
有机源调理剂
Organic conditioner (t·hm-2)
有机肥
Organic fertilizer (t·hm-2)
CK0 无调理剂、不施肥Without fertilization and soil conditioner 0 0 0
CK1 无调理剂,常规施肥Without soil conditioner, traditional fertilization 0 0 0
M 矿物源调理剂+常规施肥Mineral soil conditioner + Traditional fertilization 0.75 0 0
O 有机源调理剂+常规施肥Organic soil conditioner + Traditional fertilization 0 1.2 0
MO 矿物源调理剂+有机源调理剂+常规施肥
Mineral soil conditioner + Organic soil conditioner + Traditional fertilization
0.75 1.2 0
MF 矿物源调理剂+有机肥+常规施肥
Mineral soil conditioner + Organic fertilizer + Traditional fertilization
0.75 0 4.5
OF 有机源调理剂+有机肥+常规施肥
Organic soil conditioner + Organic fertilizer + Traditional fertilization
0 1.2 4.5
MOF 矿物源调理剂+有机源调理剂+有机肥+常规施肥
Mineral soil conditioner + Organic soil conditioner + Organic fertilizer + Traditional fertilization
0.75 1.2 4.5

Table 2

The chemical properties of soil conditioners and organic fertilizers"

pH 有机质
SOM (%)
全氮
N (%)
全磷
P2O5 (%)
全钾
K2O (%)
氧化钙
CaO (%)
氧化镁
MgO (%)
氧化硅
SiO2 (%)
矿物源调理剂Mineral conditioner 10.5 0.14 0.05 0.12 40.0 10.5 25.5
有机源调理剂Organic conditioner 10.0 20.0 0.36 0.32 0.10 20.0 8.0
有机肥Organic fertilizer 7.7 40.0 2.50 1.80 1.50

Table 3

Changes in soil chemical property, microbial biomass and rice yield caused by different soil conditioners"

处理
Treatment
pH 有机质
SOM (g·kg-1)
碱解氮
AN (mg·kg-1)
有效磷
AP (mg·kg-1)
速效钾
AK (mg·kg-1)
CK0 5.37±0.08c 22.21±1.92b 92.08±16.33b 13.35±1.83d 75.67±10.50c
CK1 5.67±0.11c 23.24±1.91b 102.28±14.99ab 16.29±1.96cd 94.67±12.22bc
M 6.24±0.32b 26.11±3.85ab 108.19±18.57ab 17.20±1.32bcd 105.33±13.01ab
O 6.64±0.22ab 27.23±3.40ab 110.16±18.80ab 18.35±4.57abcd 108.67±17.01ab
MO 6.63±0.42ab 27.66±2.99ab 117.58±5.33ab 24.19±4.27ab 117.67±18.58ab
MF 6.85±0.12a 28.98±2.36a 126.32±19.93a 22.10±4.50abc 108.33±21.50ab
OF 6.71±0.22ab 29.36±3.04a 119.06±21.21ab 23.36±3.80abc 121.00±11.79ab
MOF 6.90±0.40a 30.22±3.18a 130.56±14.09a 24.79±5.96a 128.33±8.39a
处理Treatment 微生物量碳
MBC (mg·kg-1)
微生物量氮
MBN (mg·kg-1)
交换性酸
EA (cmol·kg-1)
交换性H+
EH (cmol·kg-1)
交换性Al3+
EAl (cmol·kg-1)
水稻产量
Rice yield (t·hm-2)
CK0 246.51±90.36d 12.65±3.79e 0.80±0.07a 0.45±0.03a 0.35±0.05a 8.50±0.46c
CK1 293.11±20.98cd 19.14±4.69de 0.40±0.06b 0.16±0.03b 0.24±0.04b 9.22±0.66bc
M 299.95±49.50cd 28.09±5.11bc 0.11±0.06c 0.07±0.04c 0.04±0.02c 10.05±0.99ab
O 362.00±103.02bcd 29.56±3.32abc 0.08±0.03c 0.04±0.01c 0.04±0.03c 10.11±0.75ab
MO 481.86±99.96ab 32.32±2.01ab 0.10±0.06c 0.06±0.04c 0.05±0.03c 10.56±0.79ab
MF 497.06±43.94ab 23.15±6.12cd 0.05±0.02c 0.03±0.02c 0.02±0.01c 10.25±0.75ab
OF 397.91±42.25bc 25.53±1.23bcd 0.09±0.03c 0.07±0.02c 0.02±0.01c 10.64±0.56ab
MOF 599.62±95.37a 36.43±3.23a 0.06±0.03c 0.03±0.03c 0.03±0.02c 11.11±0.99a

Table 4

Effects of different soil conditioners on soil enzyme activities and ecological stoichiometry"

处理
Treatment
β-葡萄糖苷酶
BG (nmol·g-1·h-1)
纤维二糖水解酶
CB (nmol·g-1·h-1)
β-N-乙酰氨基葡萄糖苷酶NAG (nmol·g-1·h-1) 亮氨酸氨基酞酶
LAP (nmol·g-1·h-1)
酸性磷酸酶
PHOS (nmol·g-1·h-1)
CK0 98.82±8.17a 6.52±1.03a 31.23±4.70a 8.42±0.52a 156.60±6.92a
CK1 82.22±7.50bc 4.69±0.59b 33.64±12.34a 7.57±1.01ab 132.28±2.50bc
M 81.79±10.04bc 4.71±0.78b 35.03±11.65a 7.66±0.92ab 130.95±12.77bc
O 82.35±8.41bc 4.58±0.66b 35.64±9.66a 7.21±0.88ab 123.37±9.91bc
MO 97.38±6.69ab 5.07±1.00b 40.48±7.84a 7.34±0.62ab 136.97±4.90b
MF 74.21±8.78c 4.21±0.48b 39.66±7.96a 6.63±0.65b 109.00±5.99de
OF 76.80±9.89c 3.97±0.92b 41.99±11.88a 6.31±0.69b 104.57±4.76e
MOF 95.46±9.17ab 4.37±0.85b 42.97±9.55a 6.72±0.34b 119.86±9.60cd
处理
Treatment
酶碳氮比
Enzyme C/N
酶碳磷比
Enzyme C/P
酶氮磷比
Enzyme N/P
向量长度
Vector length
向量角度
Vector angle (°)
CK0 1.27±0.06a 0.92±0.02b 0.73±0.03b 0.83±0.03a 61.03±1.50c
CK1 1.22±0.10a 0.91±0.02b 0.75±0.07ab 0.79±0.05a 59.81±3.04bc
M 1.20±0.05a 0.91±0.02b 0.77±0.05ab 0.78±0.02a 59.41±2.48ab
O 1.20±0.09a 0.93±0.03ab 0.78±0.04ab 0.79±0.07a 58.31±0.52ab
MO 1.20±0.05a 0.94±0.01ab 0.78±0.04ab 0.81±0.03a 57.89±1.74ab
MF 1.14±0.06a 0.93±0.02ab 0.81±0.03ab 0.76±0.05a 56.42±1.28ab
OF 1.14±0.11a 0.94±0.02ab 0.83±0.06a 0.76±0.08a 55.11±2.60ab
MOF 1.18±0.04a 0.96±0.00a 0.81±0.03ab 0.81±0.02a 55.83±1.12a

Fig. 1

Redundant analysis between soil physicochemical properties and soil enzyme activities NAG: β-1,4-N-acetylglucosidase; PHOS: Acid phosphatase. *: P<0.05, **: P<0.01, ***: P<0.001"

Fig. 2

Correlations among rice yield, soil chemical properties and enzyme activities"

Fig. 3

Structural equation modeling showing the driving factors (A) and pathway on rice yield and standardized total effects (B) Black and red arrows indicate positive and negative correlations, respectively. Line width reflects the correlation coefficient"

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