花后高温胁迫对小麦氮素同化利用及产量形成的影响

doi:10.3864/j.issn.0578-1752.2025.19.004

Abstract

Abstract:

【Objective】This study aimed to investigate the impact of post-anthesis heat stress on yield formation and nitrogen assimilation capacity in wheat genotypes differing in heat tolerance, so as to provide a theoretical foundation for resilient and stable-yielding cultivation strategies.【Method】Field experiments were conducted from 2022 to 2024 at the National High-Tech Agricultural Park of Anhui Agricultural University using a split-plot design. Temperature was set as the main factor with two levels, including high temperature stress (HT) and ambient control (CK), while three nitrogen rates (no nitrogen (N0), 112.5 kg·hm^-2 (N1), and 225.0 kg·hm^-2 (N2)) and six wheat cultivars (three heat-tolerant: HM33, LK1109, AN1589; three heat-sensitive: FM5, TN19, WK1702) were arranged as subplot factors. High temperature stress was applied after anthesis. Grain yield, photosynthetic parameters (LI-6800), and activities of key nitrogen metabolism enzymes (spectrophotometry) were determined.【Result】High-temperature stress after anthesis significantly inhibited wheat growth, development, and physiological metabolism, leading to a marked decrease in yield, dry matter weight, relative chlorophyll content (SPAD), leaf area index (LAI), net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), nitrate reductase (NR) activity, and glutamine synthetase (GS) activity, as well as nitrogen accumulation, nitrogen use efficiency, nitrogen absorption efficiency, and nitrogen fertilizer use efficiency. The yield reduction due to high-temperature stress exhibited significant varietal heat tolerance differences and nitrogen application effects: the two-year average yield reduction of heat-tolerant varieties of HM33, LK1109, and AN1589 under N0, N1, and N2 treatments were 9.71%-6.13%, 9.91%-6.24%, and 11.87%-6.42%, respectively, lower than the reductions in sensitive varieties of FM5, TN19, and WK1702, which were 15.26%-10.38%, 12.56%-9.84%, and 12.93%-11.17%, respectively. In terms of physiological mechanisms, high-temperature stress after anthesis significantly reduced P_n across all nitrogen treatments, with the reduction showing a decreasing gradient as nitrogen levels increased: N0>N1>N2. The changes in G_s and T_r followed the same pattern as P_n. Furthermore, high-temperature stress significantly reduced NR and GS activity in the flag leaves across all nitrogen treatments, but increased nitrogen fertilization effectively and alleviated the decline in enzyme activity, with the highest enzyme activity observed under the N2 treatment. The reduction in nitrogen use efficiency under high-temperature stress also showed varietal differences: the nitrogen use efficiency of the heat-tolerant variety HM33 decreased the least under the N2 treatment (by 20.00%), while the sensitive variety FM5 exhibited the greatest decrease (31.55%) under the same treatment.【Conclusion】Post-flowering high temperature stress significantly reduced wheat yield, while the application of nitrogen fertilizers (112.5 and 225.0 kg·hm^-2) effectively mitigated this decline. Heat-tolerant varieties minimized yield losses by maintaining the activity of key nitrogen-metabolizing enzymes and optimizing nitrogen assimilation and transport. Increased nitrogen application, particularly at the 225.0 kg·hm^-2, significantly enhanced nitrogen use efficiency and promoted the accumulation of assimilates in grains. These results demonstrated that combining heat-tolerant genotypes with appropriate nitrogen management could improve thermotolerance in wheat, which provided a theoretical foundation for cultivating high and stable yields under heat stress.

Key words: wheat, high temperature stress, nitrogen application rate, nitrogen use efficiency, yield

LEI BiXin, YU YongBo, ZHANG MingTong, CUI GuoJi, HONG JiaWen, HU Tao, YOU AiXin, ZHANG WenJing, MA ShangYu, HUANG ZhengLai, FAN YongHui. Impact of Post-Anthesis Heat Stress on Nitrogen Use Efficiency and Yield Components in Wheat[J].Scientia Agricultura Sinica, 2025, 58(19): 3837-3856.

Figures/Tables 11

Table 1

Fig. 1

Table 2

Effects of post-anthesis high temperature stress on wheat yield and yield components"

年份 Year	品种 Cultivar	处理 Treatment	穗数 Spike number (×10⁴·hm^-2)	穗粒数 Grain number	千粒重 1000-grain weight (g)	产量 Yield (kg·hm^-2)
2022—2023	淮麦33 HM33	CKN0	412.67±0.33b	35.00±0.58c	41.57±0.29b	6002.69±61.26e
		CKN1	426.00±2.89a	39.33±0.88b	43.78±0.60a	7332.13±97.57c
		CKN2	433.67±4.41a	44.00±0.58a	44.17±0.61a	8423.64±36.59a
		HTN0	409.33±0.33b	35.00±0.58c	39.00±0.48c	5587.17±115.31e
		HTN1	432.67±2.19a	39.33±0.33b	40.22±0.05bc	6843.79±59.90d
		HTN2	435.67±2.33a	43.33±0.33a	42.26±0.34ab	7977.91±100.50b
	龙科1109 LK1109	CKN0	458.33±4.41b	33.67±0.33c	34.78±0.19c	5365.67±38.42e
		CKN1	520.00±1.15a	37.33±0.33b	37.60±0.25b	7300.37±98.38c
		CKN2	525.67±1.20a	40.00±0.58ab	39.23±0.28a	8247.23±73.81a
		HTN0	463.67±4.10b	33.00±0.58c	32.57±0.25d	4983.97±116.14f
		HTN1	518.33±2.03a	37.67±0.58ab	34.40±0.08c	6715.17±22.63d
		HTN2	521.33±3.18a	41.00±0.58a	36.68±0.14b	7839.32±52.21b
	安农1589 AN1589	CKN0	412.00±2.00c	32.67±0.33c	40.41±0.27b	5438.75±60.75e
		CKN1	425.33±3.18b	35.67±0.33b	42.49±0.24a	6444.22±33.30c
		CKN2	504.33±2.33a	38.67±0.67a	42.89±0.38a	8361.77±81.84a
		HTN0	411.67±1.45c	32.33±0.33c	36.79±0.16d	4896.53±18.61f
		HTN1	433.33±1.67b	35.33±0.33b	38.72±0.23c	5928.12±60.76d
		HTN2	501.00±2.31a	38.33±0.33a	40.75±0.38b	7824.58±37.19b
	泛麦5号 FM5	CKN0	400.00±2.08c	35.00±0.58b	38.53±0.19a	5394.27±94.91e
		CKN1	431.67±3.33b	39.00±0.58a	39.54±0.09a	6654.90±74.84c
		CKN2	482.33±2.67a	40.67±0.33a	39.94±0.42a	7832.87±40.12a
		HTN0	393.33±1.67c	33.67±0.88b	34.47±0.88b	4558.39±32.29f
		HTN1	430.67±0.67b	39.00±0.58a	34.56±0.36b	5804.81±89.32d
		HTN2	482.67±3.93a	40.33±0.67a	35.72±0.28b	6950.52±45.39b
	泰农19 TN19	CKN0	416.67±4.41c	34.67±0.88c	37.46±0.66b	5405.86±15.07d
		CKN1	430.00±1.15bc	41.33±0.33b	38.40±0.32b	6823.33±16.57b
		CKN2	448.33±1.67a	43.67±0.33a	40.31±0.38a	7890.45±76.93a
		HTN0	423.33±4.41c	33.33±0.67c	34.04±0.13c	4802.33±91.17e
		HTN1	428.00±1.53bc	41.00±0.00b	34.60±0.05c	6071.70±17.69c
		HTN2	442.33±5.36ab	43.00±0.58ab	37.17±0.07b	7071.21±133.9b
	皖垦1702 WK1702	CKN0	417.00±1.00b	35.00±0.58b	38.75±0.26ab	5654.77±44.27e
		CKN1	461.33±1.76a	39.67±0.33a	39.65±0.09ab	7255.52±49.71b
		CKN2	465.00±2.65a	40.67±0.88a	40.87±1.04a	7720.82±44.12a
		HTN0	413.33±3.28b	34.33±0.33b	35.14±0.06d	4986.85±89.52f
		HTN1	455.33±5.93a	38.67±0.33a	36.14±0.29cd	6363.77±133.80d
		HTN2	455.67±7.54a	39.33±0.67a	38.01±0.17bc	6810.76±137.57c
2023—2024	淮麦33 HM33	CKN0	403.00±0.58c	35.00±0.58c	40.23±0.66c	5671.36±10.82e
		CKN1	421.33±1.86b	38.00±0.58b	43.16±0.83ab	6906.86±54.24c
		CKN2	448.67±1.76a	44.00±0.58a	44.29±0.64a	8747.42±253.63a
		HTN0	400.00±2.31c	34.67±0.88c	35.77±0.59d	4962.18±190.42f
		HTN1	418.33±1.20b	37.33±0.33b	39.81±0.61c	6216.14±67.09d
		HTN2	448.00±1.15a	43.00±0.58a	42.24±0.27b	8138.20±169.86b
	龙科1109 LK1109	CKN0	433.00±0.58c	33.33±0.33b	39.19±0.65c	5655.99±31.03e
		CKN1	496.33±1.33b	35.33±0.33b	41.03±0.74b	7196.21±126.63c
		CKN2	519.00±2.31a	40.00±0.58a	42.97±0.74a	8923.33±210.22a
		HTN0	431.67±1.45c	33.00±0.58b	34.67±1.01d	4937.71±96.83f
		HTN1	495.00±2.31b	34.33±0.67b	38.06±0.89c	6469.13±173.86d
		HTN2	517.67±1.33a	39.00±0.58a	40.88±0.81b	8251.40±58.26b
	安农1589 AN1589	CKN0	410.33±0.88c	33.00±0.58bc	39.85±0.57c	5397.43±139.56d
		CKN1	424.07±2.31b	35.00±0.58b	41.38±0.39b	6142.98±137.91c
		CKN2	445.56±3.53a	39.00±0.58a	43.66±0.48a	7585.08±81.60a
		HTN0	404.80±2.91c	31.33±0.33c	36.70±0.45d	4654.63±85.43e
		HTN1	421.14±1.00b	34.00±1.53b	39.29±0.44c	5629.55±297.32d
		HTN2	443.81±2.03a	38.00±0.58a	42.10±0.40b	7098.49±39.04b
	泛麦5号 FM5	CKN0	402.87±1.33c	34.00±0.58b	38.54±0.39b	5279.13±107.51d
		CKN1	423.33±0.33b	39.67±1.86a	39.62±0.18ab	6652.94±306.09b
		CKN2	446.89±2.67a	41.00±1.15a	40.53±0.26a	7425.04±199.81a
		HTN0	401.53±2.67c	33.00±1.15b	33.90±0.61d	4485.78±79.58e
		HTN1	422.88±1.33b	39.33±0.88a	35.55±0.45c	5909.63±112.43c
		HTN2	445.81±2.19a	40.00±1.53a	37.70±0.11c	6719.96±230.76b
	泰农19 TN19	CKN0	414.87±1.33c	33.00±0.58c	38.39±0.65bc	5255.66±124.53d
		CKN1	421.54±1.33b	43.67±0.88ab	39.53±0.39ab	7276.08±143.18b
		CKN2	434.88±1.33a	44.67±0.88a	40.03±0.20a	7776.84±169.99a
		HTN0	413.54±1.33c	32.00±0.58c	34.19±0.45d	4522.50±59.51e
		HTN1	420.21±2.31b	42.00±1.00b	37.08±0.54c	6544.64±182.77c
		HTN2	432.22±2.31a	43.67±0.67ab	37.38±0.65c	7054.41±156.49b
	皖垦1702 WK1702	CKN0	420.21±2.31c	36.00±0.58b	37.54±0.49c	5676.01±35.50d
		CKN2	432.22±2.31b	40.00±0.58a	40.17±0.38b	6943.89±71.83b
		CKN1	446.89±3.53a	41.67±0.33a	43.95±0.68a	8180.68±2.93a
		HTN0	418.88±1.33c	35.67±0.33b	32.65±0.66e	4878.76±128.01e
		HTN1	429.55±1.33b	39.67±0.88a	35.79±0.64d	6101.30±225.0.84c
		HTN2	445.56±2.67a	41.00±0.58a	40.05±0.37b	7317.06±166.20b
2022—2023	F值 F-value	温度 Temperature	819.10**	45.99**	4729.13**	1076.15**
		氮肥 Nitrogen	232.02**	219.46**	164.83**	4.76**
		温度×氮肥Temperature×Nitrogen	345.65**	3.15**	123.94**	40.19**
2023—2024		温度 Temperature	3353.76**	585.14**	76.84*	60.78*
		氮肥 Nitrogen	1049.74**	5.66*	9.40**	12.17**
		温度×氮肥 Temperature×Nitrogen	1476.43**	28.24**	8.37*	34.09**

Table 2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Table 3

Effects of post-anthesis high temperature stress on nitrogen use efficiency in wheat"

品种 Cultivar	处理 Treatment	氮积累量 Nitrogen accumulation (kg·hm^-2)	氮素利用率 Nitrogen use efficiency (kg·kg^-1)	氮素吸收效率 Nitrogen uptake efficiency (%)	氮肥利用率 Nitrogen recovery efficiency (%)
淮麦33 HM33	CKN0	103.67±2.17e	—	—	—
	CKN1	153.79±1.63c	15.80±0.76b	27.20±0.48c	35.71±1.16a
	CKN2	188.57±2.06a	19.31±1.00a	37.15±0.54a	33.58±0.18b
	HTN0	92.59±2.72f	—	—	—
	HTN1	127.61±3.96d	12.11±0.76c	22.57±0.74d	24.95±1.02c
	HTN2	165.98±5.81b	15.45±0.30b	32.69±1.14b	29.03±1.75d
龙科 1109 LK1109	CKN0	104.81±4.73e	—	—	—
	CKN1	142.88±7.30c	17.94±0.33b	25.27±1.20c	27.13±2.75a
	CKN2	188.93±4.30a	21.26±1.02a	37.21±0.75a	33.27±0.52b
	HTN0	95.65±2.58f	—	—	—
	HTN1	132.35±4.82d	12.74±1.10d	20.24±3.67d	26.15±2.30b
	HTN2	166.36±2.42b	15.98±0.76c	32.77±0.41b	27.97±0.28b
安农1589 AN1589	CKN0	169.34±15.72cd	—	—	—
	CKN1	214.41±15.75b	14.01±0.53b	37.91±2.53b	32.12±0.98a
	CKN2	253.52±12.26a	20.76±0.75a	49.93±2.24a	33.29±1.38a
	HTN0	118.14±8.84e	—	—	—
	HTN1	154.87±8.46d	10.14±0.15c	31.05±1.61c	26.17±0.31b
	HTN2	186.94±9.36c	15.42±0.73b	42.71±3.68b	27.21±0.37b
泛麦5号FM5	CKN0	156.26±7.32d	—	—	—
	CKN1	204.04±5.08b	16.75±1.21b	36.09±0.64b	34.04±1.81a
	CKN2	240.70±11.14a	21.57±1.17a	47.41±2.01a	33.39±1.53a
	HTN0	104.50±7.99e	—	—	—
	HTN1	141.99±8.45d	10.41±0.28d	25.11±1.38c	26.72±0.43b
	HTN2	178.10±9.86c	13.90±0.51c	35.08±1.84b	29.11±0.94b
泰农19 TN19	CKN0	126.34±7.60c	—	—	—
	CKN1	172.85±5.12b	15.03±0.35b	30.57±0.82c	33.14±2.24a
	CKN2	209.33±8.44a	17.75±0.57a	41.23±1.67a	32.82±0.65a
	HTN0	94.06±4.95d	—	—	—
	HTN1	130.23±5.09c	10.12±0.17d	23.03±0.76d	25.77±1.54c
	HTN2	167.72±6.25b	12.14±0.53c	33.04±1.17b	29.13±0.60b
皖垦1702 WK1702	CKN0	137.08±6.85d	—	—	—
	CKN1	183.25±5.32b	17.22±0.75b	32.41±0.96b	32.90±1.21a
	CKN2	219.42±5.32a	21.83±0.90a	43.22±1.06a	32.57±0.72a
	HTN0	89.40±3.80f	—	—	—
	HTN1	124.69±6.70e	11.17±1.76d	22.88±0.47c	25.15±2.56c
	HTN2	162.74±0.53c	14.94±1.17c	32.06±0.20b	29.01±1.66b
F-value	温度 Temperature	1.64	127.18**	0.78	7.86
	氮肥 Nitrogen	30.80**	116.16**	0.58	203.04**
	温度×氮肥Temperature×Nitrogen	55.93**	6.18	162.60**	4.90

Table 3

Fig. 8

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年份 Year	全氮 Total N (g·kg^-1)	有机质 Organic matter (g·kg^-1)	有效磷 Available phosphorous (mg·kg^-1)	碱解氮 Alkali hydrolyzed nitrogen (mg·kg^-1)	速效钾 Rapidly-available potassium (mg·kg^-1)
2022—2023	1.28	13.64	31.43	73.63	210.59
2023—2024	0.98	10.65	33.67	61.97	212.79

Impact of Post-Anthesis Heat Stress on Nitrogen Use Efficiency and Yield Components in Wheat

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