Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (4): 704-718.doi: 10.3864/j.issn.0578-1752.2025.04.007

• PLANT PROTECTION • Previous Articles     Next Articles

Genome-Wide Identification and Expression Analysis of HMG Family Genes in Botrytis cinerea

ZHANG TianYu(), LI Bai, ZANG JinPing, CAO HongZhe, DONG JinGao, XING JiHong(), ZHANG Kang()   

  1. College of Life Sciences, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei
  • Received:2024-10-17 Accepted:2024-12-10 Online:2025-02-16 Published:2025-02-24
  • Contact: XING JiHong, ZHANG Kang

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Abstract:

【Objective】High mobility group protein (HMG), as a widespread eukaryotic protein, plays an important role in the regulation of fungal growth and development. This study aims to identify the HMG family members of Botrytis cinerea through the genome-wide identification, and analyze their expression rules during the growth and development, infection and response to stress, so as to lay a foundation for further investigation of the function of this family gene in the growth and development and pathogenic mechanism of B. cinerea.【Method】HMMER software was used to screen out possible HMG gene IDs in B. cinerea protein database, and the online tools Pfam and SMART were used to verify the possible HMG family members. Using ProtParam, TBtools, MEME, MEGA, MCScanX and other tools, the physicochemical characteristics, chromosome localization, gene structure, phylogenetic tree and collinearity of B. cinerea HMG gene family members were analyzed. Based on transcriptome sequencing data of B. cinerea during conidial development and infection, the expression of HMG family genes in B. cinerea during conidial development and infection was analyzed by using TBtools software. In addition, the expression of HMG family genes in B. cinerea under NaCl, KCl, H2O2 and other stresses was observed by real-time quantitative PCR (qPCR).【Result】Nine HMG family members were identified in B. cinerea, the number of encoded amino acids ranged from 101 to 1076, and the molecular weight ranged from 11.48 to 120.64 kDa. They could be divided into HMGA and HMGB subfamilies, which had conserved HMG domain, distributed on six chromosomes, and had gene collinearity with Fusarium graminearum and Fusarium oxysporum. The expression pattern analysis showed that the expression levels of this family gene showed obvious changes during conidial development and B. cinerea infection. Among them, Bcin01g08840, Bcin07g04610, Bcin11g04340 and Bcin11g03320 continued to be highly expressed during conidial development. The expression levels of Bcin07g04610, Bcin11g04340, Bcin11g05790 and Bcin01g08840 were significantly down-regulated during B. cinerea infection. qPCR analysis showed that the gene expression of this family was significantly down-regulated after NaCl and KCl stress treatment.【Conclusion】B. cinerea contains nine HMG family members, which can be divided into HMGA and HMGB subfamilies, the members distribute on six chromosomes, and have different expression patterns in the process of conidial development, infection and stress response of the pathogen. It is speculated that the family genes play an important role in the growth and development, pathogenicity and stress response of B. cinerea.

Key words: Botrytis cinerea, HMG family gene, genome-wide, gene expression, bioinformatics, stress

Table 1

Primers used for qPCR"

基因名称 Gene name 引物序列 Primer sequence (5′→3′)
Bcin07g04610 F: GAATGGGAAGGCAAGCACAC R: CGCCTCTATTGACTCGCCAT
Bcin09g02870 F: GCGCGGTGATTGCTATTGAG R: TCCTGCATCTCAGAATCCGC
Bcin11g03320 F: AACAAGGCTGTCAATGCACG R: CGTTGCTCAGGCGACATAGA
Bcin05g01760 F: TCTACATGCAAACCGCTCGT R: CTACGAGTTGAGCATCGGCA
Bcin02g06030 F: CCGCATACCATCTACCCCAC R: GCTCCTGATACCTCTTCGCC
Bcin11g05790 F: CTGTGGTGATAGTTGGCCGT R: CTGCATGGGTCTCTGTTGGT
Bcin01g08840 F: ACAGGGCTACAGGGAAGGAT R: CGGCGATGCTGTTTTGAGTC
Bcin11g04340 F: TCCCCAGCAATCTTTCACCC R: TGGAAGGTGTTGATTGGGCT
Bcin11g05240 F: GCTCGCCATCCTCCAATACA R: GCAGAAAGTCCACGCTTTGG
β-tubulin F: ACATGCTCTGCCATTTTCCG R: TTGTTAGGGATCCACTCAACGAAG

Table 2

Physicochemical characteristics of the HMG family genes in B. cinerea"

基因名称
Gene name		 起始位点
Initiation site		 终止位点
Termination site		 CDS (bp) 氨基酸数目
Number of amino acids (aa)		 分子量
Molecular weight (Da)		 pI
Bcin01g08840 3070697 3074891 1629 542 59264 8.46
Bcin02g06030 2131709 2133995 2100 699 78297 8.98
Bcin05g01760 667383 669885 1026 341 36349 8.39
Bcin07g04610 1655881 1659887 3231 1076 120642 6.18
Bcin09g02870 1026663 1029821 2403 800 89042 9.39
Bcin11g03320 1138331 1139915 1008 335 37277 10.16
Bcin11g05240 1867672 1869358 306 101 11476 9.22
Bcin11g05790 2042942 2046139 1617 538 61800 6.49
Bcin11g04340 1606589 1608013 675 224 24949 7.76

Fig. 1

Phylogenetic tree of HMG gene family proteins"

Fig. 2

Phylogenetic analysis of HMG family proteins"

Fig. 3

Chromosomal localization of HMG family genes in B. cinerea"

Fig. 4

Collinearity relationships of HMG family genes in B. cinerea, F. oxysporum and F. graminearum"

Fig. 5

Gene structure analysis of HMG family genes in B. cinerea"

Fig. 6

Structural analysis of HMG family proteins in B. cinerea"

Fig. 7

Analysis of the conserved motifs of HMG family proteins in B. cinerea"

Fig. 8

Interaction protein prediction of B. cinerea HMG family proteins"

Fig. 9

Expression of HMG family genes during conidial development of B. cinerea"

Fig. 10

Expression of HMG family genes during B. cinerea infecting A. thaliana"

Fig. 11

Effects of stress treatment on the expression of HMG family genes in B. cinerea"

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