马铃薯StCYP83基因家族鉴定及其抗晚疫病的功能分析

doi:10.3864/j.issn.0578-1752.2023.16.007

Abstract

Abstract:

【Objective】The objective of this study is to identify StCYP83 gene family in potato and analyze their expression patterns in response to Phytophthora infestans infection, so as to mine the StCYP83 genes with potential function in resistance to late blight and provide novel resistance gene resources for molecular resistance breeding in potato.【Method】The members of StCYP83 gene family were identified by bidirectional BLAST method. The basic information of StCYP83 protein sequence, subcellular localization and conserved motifs were analyzed by ExPASy Prot Param, Cell-Ploc 2.0 and ESPript, etc. The qRT-PCR was used to analyze the expression pattern of StCYP83 genes in response to P. infestans infection. The immune function of candidate gene StCYP83B1 against P. infestans was analyzed in either Agrobacterium tumefaciens-mediated transient transformation in leaves of Nicotiana benthamiana or stably transformed potato lines with overexpression (OE) of StCYP83B1.【Result】A total of 10 StCYP83 genes were identified in the potato genome, which were named StCYP83B1-StCYP83B10, respectively, with the encoded protein lengths ranging from 387 to 503 aa and molecular weights ranging from 44 to 57 kDa. The subcellular localization of StCYP83 proteins was predicted in the endoplasmic reticulum. The qRT-PCR confirmed that members of StCYP83 could be induced in response to P. infestans infection, suggesting that StCYP83 genes might play a role in the interaction between potato and P. infestans. Accordingly, StCYP83B1 with the highest homology to AtCYP83B1 was selected as a candidate gene for subsequent immune functional analysis. The pathogenicity assay on N. benthamiana leaves showed that overexpression of StCYP83B1 could enhance plant resistance against P. infestans. In accordance with this, overexpression of StCYP83B1 could significantly promote the up-regulation expression of PTI marker genes (NbWRKY7 and NbWRKY8), SA signaling marker genes (NbPR1 and NbPR2), JA signaling marker genes (NbPR3 and NbLOX) and enhance the reactive oxygen species (ROS) burst induced by flg22. In addition, cysteine site in the conserved motif of StCYP83B1 protein was required for its immune function. StCYP83B1 overexpression (StCYP83B1-OE) lines showed enhanced resistance to P. infestans. In accordance with this, StCYP83B1-OE could enhance PTI immune responses, including the increased level of ROS induced by flg22 and the significantly up-regulated expression of PTI marker genes (StWRKY7, StWRKY8 and StACRE31) as well as SA-mediated signaling marker genes (StPR1, StPR2, StPR5 and StPAL2) and JA-mediated signaling marker genes (StLOX, StAOS and StOPR3) in response to P. infestans infection.【Conclusion】A total of 10 members of StCYP83 family were identified, which could be induced by P. infestans infection in different degrees. StCYP83B1 regulates plant resistance to P. infestans by activating PTI, SA and JA signaling pathways. The cysteine site in the heme binding domain of StCYP83B1 is required for its immune function.

Key words: potato (Solanum tuberosum), StCYP83 gene family, Phytophthora infestans, plant resistance, late blight

KONG LeHui, ZONG DeQian, SHI QingYao, YIN PanPan, WU WenYu, TIAN Peng, SHAN WeiXing, QIANG XiaoYu. Identification of StCYP83 Gene Family in Potato and Analysis of Its Function in Resistance Against Late Blight[J].Scientia Agricultura Sinica, 2023, 56(16): 3124-3139.

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基因名 Gene name	基因ID Gene ID	蛋白长度 Length (aa)	分子量 Molecular weight (Da)	等电点 pI
StCYP83B1	PGSC0003DMG400016616	496	56706.30	9.00
StCYP83B2	PGSC0003DMG400031519	491	56113.44	8.53
StCYP83B3	PGSC0003DMG401031520	497	57164.85	8.85
StCYP83B4	PGSC0003DMG402031520	496	56749.34	9.10
StCYP83B5	PGSC0003DMG400011325	476	54658.53	8.79
StCYP83B6	PGSC0003DMG401021057	420	47673.35	5.45
StCYP83B7	PGSC0003DMG400026778	503	57072.21	7.12
StCYP83B8	PGSC0003DMG402021057	461	52325.37	9.00
StCYP83B9	PGSC0003DMG400006024	473	54683.80	7.56
StCYP83B10	PGSC0003DMG400019899	387	44732.72	6.63

Identification of StCYP83 Gene Family in Potato and Analysis of Its Function in Resistance Against Late Blight

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