小麦叶锈菌效应蛋白Pt18906激发TcLr27+31的双层防御反应

doi:10.3864/j.issn.0578-1752.2020.12.006

Abstract

Abstract:

【Objective】 Wheat leaf rust caused by Puccinia triticina (Pt) is one of the main diseases affecting wheat production. In the process of interaction between wheat and Pt, the Pt will secrete some effector proteins to the host cells to regulate the host and play a virulent function. The objective of this study is to carry out the research on Pt effector proteins, explore the pathogenic mechanism of Pt, and to provide a basis for the continuous prevention and control of diseases. 【Method】 The cDNA of Pt 13-5-72 interacting with susceptible variety Thatcher was used as template to amplify effector protein Pt18906. Sequence features of Pt18906 were analyzed using SignalP 4.1, TargetP 1.1, TMHMM 2.0 and EffectorP 2.0 software. The online software Swiss-Model was used to predict the tertiary structure of Pt18906. The secondary structure of Pt18906 was predicted by online software SOPMA. The expression pattern of Pt18906 was analyzed by RT-qPCR. The verification that Pt18906 inhibited Bax and INF1-induced programmed cell death (PCD) was conducted using Nicotiana benthamiana heterogeneous expression system. The secretion function of Pt18906 signal peptide was verified by the yeast system. The deletion mutation of Pt18906 was used to identify the virulence function motif. Subcellular localization of Pt18906 was analyzed using transient expression of Pt18906-GFP in N. benthamiana cells. Transient expression technology was used to carry out the avirulent function of Pt18906 on a full set of near-isogenic lines that did not contain disease resistance genes and contained different disease resistance genes with Thatcher as the background. The transient transformation of wheat mediated by the TTSS (Type III secretion system) was used to analyze the activity of regulating the host defense response of Pt18906. 【Result】 A Pt effector Pt18906, significantly upregulated during the interaction between Pt and Thatcher, was obtained from the transcriptome library of interaction between Pt and Thatcher. The full-length ORF sequence with 672 bp was amplified in the cDNA of wheat leaves inoculated by Pt strain 13-5-72. The effector protein lacks known functional domains and motifs. Its working environment is alkaline. Pt18906 could inhibit the PCD induced by Bax from mice and INF1 from Oomycetes by transient expression in N. benthamiana cells, which indicates that the effector protein has virulent function. By constructing deletion mutants, it is clear that the 28-47 amino acids have an important role in its virulent function. The effector protein is located in the nucleus and cytoplasm, which indicates that it acts within the cells. Pt18906 could cause allergic necrosis in the disease-resistant varieties of single gene lines TcLr27+31 and TcLr42, indicating that the effector protein is avirulent. Pt18906 could cause the accumulation of reactive oxygen species (ROS) and callose in TcLr27+31 through transiently expressing using a bacterial type III secretion system. The callose increased gradually with the injection time, and ROS reached the highest level at 10 min after injection. 【Conclusion】 The main functional domain of Pt18906 is 28-47 amino acids. Pt18906 can trigger the two-layer defense reaction accompanied with the accumulation of callose in wheat TcLr27+31 and oxidative burst.

Key words: Puccinia triticina (Pt), wheat leaf rust, effector protein, callose, reactive oxygen species (ROS), pathogenicity, defense reaction

QI Yue,LÜ JunYuan,ZHANG Yue,WEI Jie,ZHANG Na,YANG WenXiang,LIU DaQun. Puccinia triticina Effector Protein Pt18906 Triggered Two-Layer Defense Reaction in TcLr27+31[J].Scientia Agricultura Sinica, 2020, 53(12): 2371-2384.

Figures/Tables 10

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Puccinia triticina Effector Protein Pt18906 Triggered Two-Layer Defense Reaction in TcLr27+31

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