Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (8): 1368-1379.doi: 10.3864/j.issn.0578-1752.2019.08.007

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of Fertilization Rate on Forage Yield and Water Use Efficiency of Artificial Grassland in an Alpine Arid Area

ZHANG XueMei,MA QianHu,ZHANG ZiLong,WANG ZiKui(),YANG HuiMin,SHEN YuYing   

  1. College of Pastoral Agriculture Science and Technology, Lanzhou University/State Key Laboratory of Grassland Agro-Ecosystem/National Demonstration Center for Experimental Grassland Science Education, Lanzhou 730020
  • Received:2018-12-03 Accepted:2019-02-22 Online:2019-04-16 Published:2019-04-26
  • Contact: ZiKui WANG E-mail:wzk@lzu.edu.cn

Abstract:

【Objective】 This study was conducted to investigate the production of artificial grassland and the response of water resource use to planting pattern and fertilization level, so as to provide the optimal planting pattern and fertilization level in the desert steppe region of the Qinghai-Tibet plateau. 【Method】 The field experiment was conducted at Jintai pasture of Wulan county, Qinghai province in 2017 and 2018. Two planting patterns were set up, namely oat/common vetch mixed sowing and sole oat, and two fertilization rates were set up, which were high rate (120 kg N·hm -2, 103 kg P2O5·hm -2) and low rate (60 kg N·hm -2, 51.5 kg P2O5·hm -2). Four treatments were completely randomized block designed. All treatments were irrigated before sowing in the spring and no irrigation during the growing season. The measured indexes included aboveground biomass, plant nitrogen content and soil water content at major growth stage; The calculated indexes included water consumption during the growing stage, dry matter water use efficiency, and crude protein water use efficiency. 【Result】 Through mixed sowing and adding fertilizer, grassland yield could be greatly improved. In 2017 and 2018, the dry matter yield of high-fertilizer treatment was 34.7% and 9.7% greater than that of low-fertilizer treatment, respectively, and the crude protein production was 41.3% and 20.4% greater, respectively. The dry matter yield of oat/common vetch mixed sowing was 14.4% and 9.2% greater than of sole oat treatment, respectively, and the crude protein yield was 74.7% and 62.9% greater, respectively. The yield of mixed sowing with high-fertilizer treatment was the highest under all treatments, the dry matter and crude protein yield at harvest were 10 251.7 and 827.2 kg·hm -2, respectively, in 2017, and 7 589.3 and 570.2 kg·hm -2, respectively, in 2018. Adding fertilizer increased the water consumption of grassland, and the water use efficiency of grassland was greatly improved. In the two growing seasons in 2017 and 2018, the water consumption of high-fertilizer treatment was 6.2% and 4.3% higher than that of low-fertilizer treatments, respectively, the water use efficiency of dry matter increased by 21.0% and 4.9%, respectively, and the water use efficiency of crude protein was increased by 30.1% and 17.4%, respectively. Mixed sowing also increased the water consumption of grassland, slightly reduced the water use efficiency of dry matter, but greatly improved the water use efficiency of crude protein. In 2017 and 2018, the water consumption of oat/common vetch mixed sowing grassland was 17.9% and 9.2% higher than that of sole oat grassland, respectively; The water use efficiency of crude protein was increased by 46.7% and 51.3%, respectively.【Conclusion】Mix sowing and rational fertilizer application could increase the forage yield and quality as well as water use efficiency of grassland by decreasing the ineffective evaporation, and enhance the utilization of soil water. Which was recommended to be applied with spring irrigation conditions in the arid alpine area, the research can provide some reference for the cultivated grassland and excellent forage production in alpine arid and semi-arid areas in China.

Key words: artificial grassland, mixture sowing, forage yield, crude protein yield, water use efficiency

Table 1

Main soil physical and chemical properties of experimental field before sowing"

土层
Layer
(cm)
有机质
Soil organic matter
(g·kg-1)
全氮
Total nitrogen
(g·kg-1)
硝态氮
Nitrate nitrogen (mg·kg-1)
速效磷
Available phosphorus (mg·kg-1)
容重
Bulk density
(g·cm-3)
田间持水量
Field capacity (cm3·cm-3)
0-10 19.92 0.75 25.82 6.11 1.44 0.278
10-20 20.90 1.02 14.68 5.43 1.48 0.292
20-30 24.74 0.75 9.09 5.56 1.63 0.269
30-40 15.30 0.49 4.83 2.93 1.60 0.273
40-60 10.60 0.21 2.76 2.18 1.46 0.270
60-100 6.73 0.15 2.53 2.12 1.55 0.263

Fig. 1

Aboveground dry biomass at flowering and maturing stages in 2017 (A) and 2018 (B) Different letters indicate significant differences at P<0.05, The same as below"

Fig. 2

Crude protein yield under different treatments at flowering and maturing stages in 2017 (A) and 2018 (B)"

Table 2

Variance analysis of dry matter and crude protein production of different treatments in maturing stage"

方差分析
Analysis of variance
燕麦干物质产量
Dry matter of oat
燕麦粗蛋白产量
Crude protein of oat
箭筈豌豆干物质产量
Dry matter of vetch
箭筈豌豆粗蛋白产量
Crude protein of vetch
总干物质产量
Total dry matter
总粗蛋白产量
Total crude protein
P ** ** ** ** ** **
F ** ** ** * ** **
P× F ** NS ** ** NS NS

Fig. 3

Dry matter partitioning in the mixture and the comparison with sole oat in 2017 (A) and 2018 (B)"

Fig. 4

Crude protein partitioning in the mixture and the comparison with sole oat in 2017 (A) and 2018 (B)"

Fig. 5

Rainfall distribution and soil moisture dynamics in different treatments in 2017 (A) and 2018 (B)"

Fig. 6

Changes of soil water content in different treatments at the sowing stage (A) and harvest (B) of the growing season in 2017"

Fig. 7

Changes of soil water content in different treatments at the sowing stage (A) and harvest (B) of the growing season in 2018"

Table 3

Water consumption and water use efficiency under different treatments"

年份
Year
处理
Treatment
耗水量
Water consumption
(mm)
干物质水分利用效率
Water use efficiency for dry matter (kg·m-3)
粗蛋白水分利用效率
Water use efficiency for
crude protein (kg·m-3)
2017 单播高肥 Sole oat with high fertilization 254.1a 3.79a 0.212b
单播低肥 Sole oat with low fertilization 219.9b 3.24b 0.155c
混播高肥 Oat/Vetch with high fertilization 277.8a 3.69a 0.298a
混播低肥 Oat/Vetch with low fertilization 281.1a 2.94c 0.237b
2018 单播高肥 Sole oat with high fertilization 353.5ab 1.90a 0.090b
单播低肥 Sole oat with low fertilization 344.8b 1.84a 0.084b
混播高肥 Oat/Vetch with high fertilization 392.0a 1.94a 0.147a
混播低肥 Oat/Vetch with low fertilization 370.2ab 1.82a 0.117ab
方差分析 Analysis of variance
P * * **
F ** ** **
P×F NS NS NS
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