白菜型冬油菜抗寒相关性状的遗传分析

doi:10.3864/j.issn.0578-1752.2016.21.003

Abstract

Abstract: 【Objective】The objective of this paper is to understand genetic way and to provide a theoretical foundation of cold resistance traits in winter rapeseed (Brassica rapa L.). 【Method】 In this study, six winter rapeseed cultivars with different cold resistances were used as parent materials to make complete diallel cross combinations with the Griffing methodⅠ. The parents and F1 generations were planted to obtain the data of cold resistance traits. By using the additive-dominant model and MINQUE(1) method, the genetic variance, genetic correlation, parent additive effects, combination dominant effects and heritability, and heterosis of over wintering rate and 11 traits in winter rapeseed were analyzed.【Result】Over wintering rate, POD activity, SOD activity, SS content, relative electrolytic leakage, chlorophyll content, transpiration rate and stomatal conductance were mainly controlled by additive effects, and dominant effects were much less important. The dominant effects prevailed in the traits of CAT activity, MDA content, SP content and net photosynthetic rate. Over wintering rate was detected to be significantly and positively correlated with CAT activity, POD activity, SOD activity, SP content, SS content, chlorophyll content, transpiration rate, stomatal conductance and net photosynthetic rate, but negatively correlated with MDA content and relative electrolytic leakage. Longyou 7 and Longyou 8 had positively additive effects in the traits of overwintering rate, CAT activity, POD activity, SOD activity, SP content, SS content, chlorophyll content, transpiration rate, stomatal conductance and net photosynthetic rate, which indicated Longyou 7 and Longyou 8 could be used as parents that improved cold resistance of generations. The results of dominant effects analysis showed that Longyou 7×GY and Longyou 7×Longyou 8 had stronger cold resistance. As for the narrow heritabilities, SOD activity, relative electrolytic leakage, transpiration rate and overwintering rate were 74.63%, 59.06%, 57.49% and 56.87%, respectively, and CAT activity was the lowest (2.83%). CAT activity, SP content and net photosynthetic rate had stronger population mean heterosis and population over-parent heterosis. 【Conclusion】Overwintering rate, SOD activity, relative electrolytic leakage and transpiration rate were used in selection at early generation. Among the 6 elite breeding lines, Longyou 7 was recommended for using as direct parental line due to its higher general combining ability in cold resistance traits.

Key words: winter rapeseed, additive effect, dominant effect, cold resistance, heterosis

LIU Lin-bo, SUN Wan-cang, LIU Zi-gang, WU Jun-yan, FANG Yan, LI Xue-cai, ZENG Xiu-cun, YANG Gang, DONG yun, CHEN Qi, FANG Yuan, YUAN Jin-hai. Genetic Analysis of Traits Related to Cold Resistance in Winter Rapeseed (Brassica rapa L.)[J].Scientia Agricultura Sinica, 2016, 49(21): 4085-4095.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2016.21.003

https://www.chinaagrisci.com/EN/Y2016/V49/I21/4085

References

[1] 刘后利. 几种芸薹属油菜的起源和进化. 作物学报, 1984, 10(1): 9-18.

Liu H L. Origin and evolution of rapeseeds. Acta Agronomica Sinica, 1984, 10(1): 9-18. (in Chinese)

[2] 孙万仓, 马卫国, 雷建民, 刘秦, 杨仁义, 武军艳, 王学芳, 叶剑, 曾军, 张亚宏, 康艳丽, 郭秀娟, 魏文惠, 杨杰, 蒲媛媛, 曾潮武, 刘红霞. 冬油菜在西北旱寒区的适应性和北移的可行性研究. 中国农业科学, 2007, 40(12): 2716-2726.

Sun W C, Ma W G, Lei J M, Liu Q, Yang R Y, Wu J Y, Wang X F, Ye J, Zeng J, Zhang Y H, Kang Y L, Guo X J, Wei W H, Yang J, Pu Y Y, Zeng C W, Liu H X. Study on adaptation and introduction possibility of winter rapeseed to dry and cold areas in north-west china. Scientia Agricultura Sinica, 2007, 40(12): 2716-2726. (in Chinese)

[3] 孙万仓, 曾秀存, 刘自刚, 杨刚, 方彦, 武军艳, 李学才. 轮回选择对白菜型冬油菜抗寒性及经济性状的影响. 中国油料作物学报, 2015, 37(4): 443-452.

Sun W C, Zeng X C, Liu Z G, Yang G, Fang Y, Wu J Y, Li X C. Effects of recurrent selection on cold tolerance and economic traits of Brassica rapa. Chinese Journal of Oil Crop Sciences,2015, 37(4): 443-452. (in Chinese)

[4] 沈金雄, 傅廷栋, 杨光圣, 马朝芝, 涂金星. 甘蓝型油菜杂种优势及产量性状的遗传改良. 中国油料作物学报, 2005, 27(1): 5-9.

Shen J X, Fu T D, Yang G S, Ma C Z, Tu J X. Analysis of heterosis reveals genetic improvement for yield traits in rapessed (Brassica napus L.). Chinese Journal of Oil Crop Sciences, 2005, 27(1): 5-9. (in Chinese)

[5] 邓武明, 阳小虎, 文凤君, 陈胜荣, 赵昌斌. 甘蓝型油菜产量性状的遗传及相关与通径分析. 中国油料作物学报, 2004, 25(4): 27-30.

Deng W M, Yang X H, Wen F J, Chen S R, Zhao C B. Inheritance model, correlation and path analysis of yield in rape (Brassica napus L.). Chinese Journal of Oil Crop Sciences,2004, 25(4): 27-30. (in Chinese)

[6] Ali M, Copeland L O, Elias S G, Kelly J D. Relationship between genetic distance and heterosis for yield and morphological traits in winter canola (Brassica napus L.). Theoretical and Applied Genetics, 1995, 91(1): 118-121.

[7] Pradhan A K, Sodhi Y S, Mukhopadhyay A, Pental D. Heterosis breeding in Indian mustard (Brassica juncea L.Czern & Coss): analysis of component characters contributing to heterosis for yield. Euphytica, 1993, 69(3): 219-229.

[8] Jiang J, Pu X, Zhang J, LI H, CHAI L, HUANG C, HU H, ZHENG B, NIU Y, JIANG L. Analysis on combining ability and heritability of the main agronomic characters for JA CMS in Brassica napus L.. Agricultural Science & Technology, 2014, 15(1): 39.

[9] 魏忠芬, 李德文, 王军, 张太平. 甘蓝型杂交油菜亲本含油量及主要农艺性状配合力与遗传力分析. 西北农业学报, 2014, 23(6): 102-108.

Wei Z F, Li D W, Wang J, Zhang T P. Analysis on combining ability and heritability of main agronomic traits and oil contents of hybrid parents in Brassica napus L.. Acta Agriculturae Boreali- occidentalis Sinica, 2014, 23(6): 102-108. (in Chinese)

[10] 姜磊, 陶诗顺, 张敏, 黄霞, 彭雅利. 甘蓝型油菜亲本主要农艺性状的配合力与遗传力分析. 西北农业学报, 2011, 3(2): 5.

Jiang L, Tao S S, Zhang M, Huang X, Peng Y L. Analysis on combining ability and genetic ability of main agronomic characters in Brassica Napus L.. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 3(2): 5. (in Chinese)

[11] 刘定富, 刘后利. 甘蓝型油菜脂肪酸成分的基因作用形式和效应. 作物学报, 1990, 16(3): 193-199.

Liu D F, Liu H L. Gene action and effects of fatty acids in Brassica napus L.. Acta Agronomica Sinica, 1990, 16(3): 193-199. (in Chinese)

[12] 戚存扣, 盖钧镒, 章元明. 甘蓝型油菜芥酸含量的主基因+多基因遗传. 遗传学报, 2001, 28(2): 182-187.

Qi C K, Gai J Y, Zhang Y M. Major gene plus poly-gene inheritance of erucic acid content in Brassica napus L.. Acta Genetic Sinica, 2001, 28(2): 182-187. (in Chinese)

[13] Burns M J, Barnes S R, Bowman J G, Clarke M H E, Werner C P, Kearsey M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus(i) Seed oil content and fatty acid composition. Heredity, 2003, 90(1): 39-48.

[14] 杨宁宁, 孙万仓, 刘自刚, 史鹏辉, 方彦, 武军艳, 曾秀存, 孔德晶, 鲁美宏, 王月. 北方冬油菜抗寒性的形态与生理机制. 中国农业科学, 2013, 47(3): 452-461.

Yang N N, Sun W C, Liu Z G, Shi P H, Fang Y, Wu J Y, Zeng X C, Kong D J, Lu M H, Wang Y. Morphological characters and physiological mechanisms of cold resistance of winter rapeseed in Northern China. Scientia Agricultura Sinica, 2013, 47(3): 452-461. (in Chinese)

[15] 刘自刚, 孙万仓, 杨宁宁, 王月, 何丽, 赵彩霞, 史鹏飞, 杨刚, 李学才, 武军艳, 方彦, 曾秀存. 冬前低温胁迫下白菜型冬油菜抗寒性的形态及生理特征. 中国农业科学, 2013, 46(22): 4679-4687.

Liu Z G, Sun W C, Yang N N, Wang Y, He L, Zhao C X, Shi P F, Yang G, Li X C, Wu J Y, Fang Y, Zeng X C. Morphology and physiological characteristics of cultivars with different levels of cold-resistance in winter rapeseed (Brassica campestris L.) during cold acclimation. Scientia Agricultura Sinica, 2013, 46(22): 4679-4687. (in Chinese)

[16] 史鹏辉, 孙万仓, 赵彩霞. 低温下抗氧化酶活性与冬油菜根细胞结冰关系的初步研究. 西北植物学报, 2013, 33(2): 329-335.

Shi P H, Sun W C, Zhao C X. Preliminary study on the relation of antioxidant enzyme activities at low temperature and the ice formation in root cells of winter rapeseed. Acta Botanica Boreali-occidentalis Sinica, 2013, 33(2): 329-335. (in Chinese)

[17] 刘自刚, 孙万仓, 方彦, 李学才, 杨宁宁, 武军艳, 曾秀存, 王月. 夜间低温对白菜型冬油菜光合机构的影响. 中国农业科学, 2015, 48(4): 672-682.

Liu Z G, Sun W C, Fang Y, Li X C, Yang N N, Wu J Y, Zeng X C, Wang Y. Effects of low nocturnal temperature on photosynthetic apparatus of winter rapeseed (Brassica campestris L.). Scientia Agricultura Sinica, 2015, 48(4): 672-682. (in Chinese)

[18] 孔德晶, 王月, 孙万仓, 曾秀存, 方彦. 鲁美宏, 杨宁宁. 北方白菜型冬油菜F₂主要生理生化特性的变异与抗寒性相关分析. 草业学报, 2014, 23(4): 79-86.

Kong D J, Wang Y, Sun W C, Zeng X C, Fang Y, Lu M H, Yang N N. Analysis of variation in physio-biochemical characteristics and cold resistance in winter rapeseed F₂ populations. Acta Prataculturae Sinica, 2014, 23(4): 79-86. (in Chinese)

[19] 朱惠霞, 孙万仓, 邓斌, 燕妮, 武军艳, 范惠玲, 叶剑, 曾军, 刘雅丽, 张亚宏. 白菜型冬油菜品种的抗寒性及其生理生化特性. 西北农业学报, 2007, 16(4): 34-38.

Zhu H X, Sun W C, Deng B, Yan N, Wu J Y, Fan H L, Ye J, Zeng J, Liu Y L, Zhang Y H. Study on cold hardiness and its physiological and biochemical characteristics of winter turnip rape (Brassica campetris). Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(4): 34-38. (in Chinese)

[20] 蒲媛媛, 孙万仓. 白菜型冬油菜抗寒性与生理生化特性关系. 分子植物育种, 2010, 8(2): 335-339.

Pu Y Y, Sun W C. The Relationship between cold resistance of winter turnip rape varieties and its physiological characteristics. Molecular Plant Breeding, 2010, 8(2): 335-339. (in Chinese)

[21] 常硕其, 邓启云, 罗祎, 陈小龙. 超级杂交稻及其亲本的耐冷性研究. 杂交水稻, 2015, 30(1): 51-57.

Chang S Q, Deng Q Y, Luo Y, Chen X L. Studies on cold tolerance of super hybrid rice and its parents. Hybrid Rice, 2015, 30(1): 51-57. (in Chinese)

[22] 贺荣华, 左佳, 李丁, 谢灵灵, 韩小霞, 高婧, 舒服, 李祺, 曹孟良. 水稻苗期抗寒性的杂种优势分析. 生命科学研究, 2011, 15(4): 356-358.

He R H, Zuo J, Li D, Xie L L, Han X X, Gao J, Shu F, Li Q, Cao M L. Analysis on the heterosis of cold tolerance at seedling stage in rice. Life Scencei Research, 2011, 15(4): 356-358. (in Chinese)

[23] 裴玉贺, 王小丽, 张恩盈, 宋希云. 玉米抗寒生理指标的遗传效应分析. 植物生理学报, 2011, 47(3): 293-297.

Pei Y H, Wang X L, Zhang E Y, Song X Y. Genetic analysis of physiological indices related to cold resistance in maize. Plant Physiology Journal, 2011, 47(3): 293-297. (in Chinese)

[24] 朱军. 数量性状的一般遗传模型和新的分析方法. 浙江农业大学学报, 1994, 20: 551-559.

Zhu J. General genetic models and new analysis methods for quantitative traits. Journal of Zhejiang Agricultura Univercity, 1994, 20: 551-559. (in Chinese)

[25] 邹奇. 植物生理学实验指导. 北京: 中国农业出版社, 2000.

Zou Q. Instruction in Plant Physiological Experiment. Beijing: China Agriculture Press, 2000. (in Chinese)

[26] 朱军. 遗传模型分析方法. 中国农业出版社, 1997.

Zhu J. New Analysis Methods for Genetic Models. Beijing: China Agriculture Press, 1997. (in Chinese)

[27] 朱军, 季道藩, 许馥华. 作物品种间杂种优势遗传分析的新方法. 遗传学报, 1993, 20(3): 262-271.

Zhu J, Ji D F, Xu F H. A genetic approach for analyzing intra-cultivar heterosis in crops. Acta Genetic Sinica, 1993, 20(3): 262-271. (in Chinese)

[28] 郭海林, 高雅丹, 薛丹丹, 陈宣, 刘建秀. 结缕草属植物抗寒性的遗传分析. 草业学报, 2009, 18(3): 53.

Guo H L, Gao Y D, Xue D D, Chen X, Liu J X. Genetic analysis of cold tolerance of zoysia grass. Acta Prataculturae Sinica, 2009, 18(3): 53. (in Chinese)

[29] 王鹏良, 徐洋, 吕智鹏, 王海燕, 覃子海, 刘建秀, 王秀娥. 假俭草杂种F₁抗寒性遗传分析. 草业学报, 2011, 20(2): 290-294.

Wang P L, Xu Y, Lü Z P, Wang H Y, Qin Z H, Liu J X, Wang X E. Genetic analysis for cold tolerance of F₁ hybrids in Eremochloa ophiuroides. Acta Prataculturae Sinica, 2011, 20(2): 290-294. (in Chinese)

[30] 张扬勇, 靳哲, 方智远, 刘玉梅, 杨丽梅, 庄木, 孙培田. 结球甘蓝抗寒性配合力分析及优良抗寒组合选育. 中国蔬菜, 2011(14): 23-27.

Zhang Y Y, Jin Z, Fang Z Y, Liu Y M, Yang L M, Zhuang M, Sun P T. Combining ability analysis of cold-resistance characteristic and elite combination breeding in cabbage (Brassica oleracea var. capitata L.). China Vegetables, 2011(14): 23-27. (in Chinese)

[31] 吴吉祥, 王国建, 朱军, 许馥华, 季道藩. 陆地棉种子性状直接效应和母体效应的遗传分析. 作物学报, 1995, 21(6): 659-664.

Wu J X, Wang G J, Zhu J, Xu F H, Ji D F. Genetic analysis on direct and maternal effects of seed traits in upland cotton (Gossypium hirsutum L.). Acta Agronomica Sinica, 1995, 21(6): 659-664. (in Chinese)

[32] 李少钦, 王健胜, 张文学, 李殿荣, 郑磊, 田建华. 甘蓝型油菜优良亲本对杂种后代产量性状的遗传效应分析. 中国油料物学报, 2011, 33(6): 545-549.

Li S Q, Wang J S, Zhang W X, Li D R, Zheng L, Tian J H. Genetic analysis for hybrid yield traits using elite parents of Brassica napus L.. Chinese Journal of Oil Crop Sciences, 2011, 33(6): 545-549. (in Chinese)

Related Articles 15

[1]	JIANG QianHong,LU ZhiFeng,ZHAO HaiYan,GUO JunJie,LIU WenBo,LING Ning,GUO ShiWei. Potential Analysis of Reducing Chemical Nitrogen Inputs While Increasing Efficiency by Organic-Inorganic Fertilization in Winter Rapeseed Producing Areas of the Middle and Lower Reaches of the Yangtze River [J]. Scientia Agricultura Sinica, 2020, 53(14): 2907-2918.
[2]	BU RongYan,CHENG Yi,WANG Hui,HAN Shang,HU RongGen,YU Zhong,LI Min,CHENG WenLong,SUN YiXiang,WANG JiaBao,WU Ji. Investigation of Present Fertilization Status and Fertilizer Utilization Rate on Rapeseed in Anhui Province [J]. Scientia Agricultura Sinica, 2020, 53(10): 2034-2044.
[3]	ZHU Yun, XU HuaLi, ZHANG YangYang, REN Tao, CONG RiHuan, LU JianWei. Yield and Nutrient Efficiency Differences of Winter Oilseed Rape Between Farmer’s Practice and Recommended Fertilization in Yangtze River Basin: Based on Large-Number of Field Experiments [J]. Scientia Agricultura Sinica, 2018, 51(15): 2948-2957.
[4]	LI HuiMin, LI WeiHua, GUO HaiPing, LIU Kun, ZHANG XiangGe, ZHANG XiaoXiang, XIE HuiLing, TANG JiHua, DING Dong. Heterosis Analysis of Internode Length Under Ear in Maize [J]. Scientia Agricultura Sinica, 2017, 50(6): 978-989.
[5]	QIN Ming, WANG HongFang, LIU ZhenGuo, WANG Ying, WANG Shuai, CHI XuePeng, LIU ChunLei, ZHANG WeiXing, XU BaoHua. Comparison of Different Cold Resistance betweenApis cerana cerana and Apis mellifera ligustica [J]. Scientia Agricultura Sinica, 2017, 50(12): 2380-2388.
[6]	WANG Li-min, ZHANG Jian-ping, DANG Zhao, PEI Xin-wu, DANG Zhan-hai. The Analysis of the Parental Combining Ability and Heterosis on Two-Line Hybrid Flax [J]. Scientia Agricultura Sinica, 2016, 49(6): 1047-1059.
[7]	GAO Meng-zhu, ZHAO Ya-lin, YAN Qing-di, ZHANG Hai-li, WANG Feng-ru, DONG Jin-gao. Analysis of the Role of the PMRP Gene in the Accumulation of Chloroplast Starch and Cold Resistance in Arabidopsis thaliana [J]. Scientia Agricultura Sinica, 2016, 49(5): 832-839.
[8]	WANG Jun-juan, WANG De-long, YIN Zu-jun, WANG Shuai, FAN Wei-li, LU Xu-ke, MU Min, GUO Li-xue, YE Wu-wei, YU Shu-xun. Identification of the Chilling Resistance from Germination Stage to Seedling Stage in Upland Cotton [J]. Scientia Agricultura Sinica, 2016, 49(17): 3332-3346.
[9]	LIU Zi-gang, SUN Wan-cang, FANG Yan, LI Xue-cai, YANG Ning-ning, WU Jun-yan, ZENG Xiu-cun, WANG Yue. Effects of Low Nocturnal Temperature on Photosynthetic Apparatus of Winter Rapeseed (Brassica campestris L.) [J]. Scientia Agricultura Sinica, 2015, 48(4): 672-682.
[10]	WEI Quan-quan, LI Lan-tao, REN Tao, WANG Zhen, WANG Shao-hua, LI Xiao-kun, CONG Ri-huan, LU Jian-wei. Diagnosing Nitrogen Nutrition Status of Winter Rapeseed via Digital Image Processing Technique [J]. Scientia Agricultura Sinica, 2015, 48(19): 3877-3886.
[11]	LIU Hai-qing, SUN Wan-cang, LIU Zi-gang, WU Jun-yan, QIAN Wu, WANG Zhi-jiang, GUO Ren-di, MA Li, HOU Xian-fei, LIU Lin-bo. Evaluation of Drought Resistance and Cold Resistance and Research of Their Relationship at Seedling Stage of Winter Rapeseed (Brassica campestris L.) in Cold and Arid Regions in North China [J]. Scientia Agricultura Sinica, 2015, 48(18): 3743-3756.
[12]	YANG Ning-Ning-1, 3 , SUN Wan-Cang-2, 3 , LIU Zi-Gang-1, 2 , 3 , SHI Peng-Hui-1, 3 , FANG Yan-1, 2 , 3 , WU Jun-Yan-2, 3 , ZENG Xiu-Cun-1, 3 , KONG De-Jing-1, 3 , LU Mei-Hong-1, 3 , WANG Yue-1, 3 . Morphological Characters and Physiological Mechanisms of Cold Resistance of Winter Rapeseed in Northern China [J]. Scientia Agricultura Sinica, 2014, 47(3): 452-461.
[13]	ZHOU Dong-Mei-1, ZHANG Ren-Zhi-1, 2 , SUN Wan-Cang-2, 3 , ZHANG Jun-1, 2 , WANG He-Ling-4. Study on Climatic Suitability for Winter Rapeseed Planting in Arid and Cold Regions in North China [J]. Scientia Agricultura Sinica, 2014, 47(13): 2541-2551.
[14]	LIU Zi-Gang, SUN Wan-Cang, YANG Ning-Ning, WANG Yue, HE Li, ZHAO Cai-Xia, SHI Peng-Fei, YANG Gang, LI Xue-Cai, WU Jun-Yan, FANG Yan, ZENG Xiu-Cun. Morphology and Physiological Characteristics of Cultivars with Different Levels of Cold-Resistance in Winter Rapeseed (Brassica campestris L.) During Cold Acclimation [J]. Scientia Agricultura Sinica, 2013, 46(22): 4679-4687.
[15]	LI Shi-Ping, CHANG Xiao-Ping, WANG Cheng-She, JING Rui-Lian. Mapping QTL for Heat Tolerance at Grain Filling Stage in Common Wheat [J]. Scientia Agricultura Sinica, 2013, 46(10): 2119-2129.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Genetic Analysis of Traits Related to Cold Resistance in Winter Rapeseed (Brassica rapa L.)

RichHTML

PDF