意大利蜜蜂工蜂幼虫饲粮的适宜亚硒酸钠添加水平

doi:10.3864/j.issn.0578-1752.2016.20.018

Abstract

Abstract: 【objective】The objective of this study is to find an appropriate level of sodium selenite which added in the food of Apis mellifera ligustica worker larvae, and to provide a theoretical basis for the nutritional needs of Se.【Method】A total of 1 440 one-day old larvae were randomly allotted into 2 batches, each batch involve 720 larvae, one of the batch was used to measure the pupation rate and eclosion rate, the other was used to measure enzyme activity and molecular index. Two batches were all randomly divided into 6 groups, the control group was fed basic diet and the treatment groups were fed different diets with sodium selenite at the levels of 0.2, 0.4, 0.6, 0.8, and 1.0 mg·kg^-1, respectively. Each group had 5 repetitions and involved 24 larvae in each. The 3-, 5- and 7-day-old larvae were used to measure larvae weight, T-AOC (total antioxidant capacity), T-SOD (total superoxide dismutase), MDA (malondialdehyde), and PO (phenoloxidase). The gene expression of SelD, SerRs, Sbp1 and Sbp2 were measured by using 5-day-old larvae. The pupation rate was calculated at 7-day-old.【Result】Compared with the control group, when the added levels of sodium selenite were 0.2-0.6 mg·kg^-1, the T-AOC was significantly increased (P＜0.05), when the added levels of sodium selenite were 0.2-0.8 mg·kg^-1, the activity of T-SOD was significantly increased (P＜0.05) and the MDA content of all the treatment groups was significantly decreased (P＜0.05). When the added level of sodium selenite was 0.4 mg·kg^-1, the activity of PO was significantly increased (P＜0.05). When the added level of sodium selenite was 0.6 mg·kg^-1, the gene expression levels of SelD, SerRs, Sbp1and Sbp2 was significantly higher than the control in 5-day-old larvae (P＜0.05). When the added level of sodium selenite was 0.4 mg·kg^-1, the weight before pupation was significantly higher than the control (P＜0.05). Compared with the control group, when the added level of sodium selenite was 0.2 mg·kg^-1, the pupation rate were significantly increased (P＜0.05). 【Conclusion】Under artificial condition, when the selenium level of basic diet was 0.21 mg·kg^-1, the appropriate level of sodium selenite which added in the food of A. m. ligusticaby the fitting curve which involve the weight before pupation and the pupation rate, and it means that the appropriate level of selenium which added in the food of A. m. ligustica worker larvae is 0.32-0.36 mg·kg^-1. worker larvae was 0.24-0.33 mg·kg^-1

Key words: Apis mellifera ligustica, worker bee larvae, sodium selenite, appropriate level

SU Zhong-qu, ZHANG Wei-xing, CHI Xue-peng, WANG Hong-fang, XU Bao-hua. Appropriate Level of Sodium Selenite for Apis mellifera ligustica Worker Bee Larvae Feed[J].Scientia Agricultura Sinica, 2016, 49(20): 4047-4055.

References

[1] Golubkina N, Sheshnitsan S, Kapitalchuk M. Ecological importance of insects in selenium biogenic cycling. International Journal of Ecology, 2014, 2014: Article ID 835636.

[2] Beck M A, Jean H, Levander O A. Host nutritional status: the neglected virulence factor. Trends in Microbiology, 2004, 12(9): 417-423.

[3] Ellis D R, Salt D E. Plants, selenium and human health. Current Opinion in Plant Biology, 2003, 6(3): 273-279.

[4] ROTRUCK J T, POPE A L, GANTHER H E, SWANSON A B, HAFEMAN D G, HOEKSTRA W G. Selenium: biochemical role as a component of glutathione peroxidase. Science, 1973, 179(4073): 588-590.

[5] Martin-Romero F J, Kryukov G V, Lobanov A V, Carlson B A, Lee B J, Gladyshev V N, Hatfield D L. Selenium metabolism in Drosophila: selenoproteins, selenoprotein mRNA expression, fertility, and mortality. The Journal of Biological Chemistry, 2001, 276(32): 29798-29804.

[6] Popham H J R, Shelby K S, POPHAM T W. Effect of dietary selenium supplementation on resistance to baculovirus infection. Biological Control, 2005, 32(3): 419-426.

[7] Laughton A M, Boots M, Siva-Jothy M T. The ontogeny of immunity in the honey bee, Apis mellifera L. following an immune challenge. Journal of Insect Physiology, 2011, 57(7): 1023-1032.

[8] Vijaya A, Rao B, Smitha S. Effects of selenium on the physiology of heart beat, oxygen consumption and growth in silkworm Bombyx mori L. American -Eurasian Journal of Toxicological Sciences, 2010, 2(4): 215-219.

[9] udas A, Hogan G R, Razniak H. Incubation temperature as a modifying factor on survival of Tenebrio molitor reared in selenium-containing media. Journal of Toxicology & Environmental Health, 1995, 44(1): 115-122.

[10] Allmang C, Wurth L, Krol A. The selenium to selenoprotein pathway in eukaryotes: More molecular partners than anticipated. Biochimica et Biophysica Acta General Subjects, 2009, 1790(11): 1415-1423.

[11] Copeland P R, Driscoll D M. Purification, redox sensitivity, and RNA binding properties of SECIS-binding protein 2, a protein involved in selenoprotein biosynthesis. The Journal of Biological Chemistry, 1999, 274(36): 25447-25454.

[12] Copeland P R, Fletcher J E, Carlson B A, Hatfield D L, Driscoll D M. A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs. The Embo Journal, 2000, 19(2): 306-314.

[13] Lescure A, Allmang C, Yamada K, CARBON P, KROL A. cDNA cloning, expression pattern and RNA binding analysis of human selenocysteine insertion sequence (SECIS) binding protein 2. Gene, 2002, 291(1/2): 279-285.

[14] Copeland P R, Stepanik V A, Driscoll D M. Insight into mammalian selenocysteine insertion: domain structure and ribosome binding properties of Sec insertion sequence binding protein 2. Molecular & Cellular Biology, 2001, 21(5): 1491-1498.

[15] Kinzy S A, Caban K, Copeland P R. Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals. Nucleic Acids Research, 2005, 33(16): 5172-5180.

[16] CABAN K, Kinzy S A, Copeland P R. The L7Ae RNA binding motif is a multifunctional domain required for the ribosome-dependent Sec incorporation activity of Sec insertion sequence binding protein 2. Molecular & Cellular Biology, 2007, 27(18): 6350-6360.

[17] Bubenik J L, Driscoll D M. Altered RNA binding activity underlies abnormal thyroid hormone metabolism linked to a mutation in selenocysteine insertion sequence-binding protein 2. The Journal of Biological Chemistry, 2007, 282(48): 34653-34662.

[18] Donovan J, CABAN K, Ranaweera R, Gonzalez-Flores J N, Copeland P R. A novel protein domain induces high affinity selenocysteine insertion sequence binding and elongation factor recruitment. The Journal of Biological Chemistry, 2008, 283(50): 35129-35139.

[19] Vandenberg J D, Shimanuki H. Technique for rearing worker honeybees in the laboratory. Journal of Apicultural Research, 1987, 26(2): 90-97.

[20] 张艳艳, 李福昌. 日粮不同硒水平对2～3月龄肉兔生长性能、抗氧化指标和肉质的影响. 动物营养学报, 2010, 22(1): 82-87.

Zhang Y Y, Li F C. Effects of different dietary selenium levels on growth performance, antioxidant indices and meat quality of meat rabbits from 2 to 3 months of age. Journal of Animal Nutrition, 2010, 22(1): 82-87. (in Chinese)

[21] 高铭宇, 杨鹰, 袁莉, 刘欣, 杨保收, 金久善, 陈越. 硒和锌相互作用及对肉用鸡胰腺抗氧化功能的影响.中国兽医科技, 2001, 31(4): 23-26.

Gao M Y, Yang Y, Yuan L, LIU X, YANG B S, JIN J S, CHEN Y. Interaction between selenium and zinc and the effect on pancreas antioxidant function of broiler. Chinese Journal of Veterinary Science and Technology, 2001, 31(4): 23-26. (in Chinese)

[22] ZHAO P, LI J, Wang Y, JIANG H. Broad-spectrum antimicrobial activity of the reactive compounds generated in vitro by Manduca sexta phenoloxidase. Insect Biochemistry & Molecular Biology, 2007, 37(9): 952-959.

[23] Kan H, Kim C H, Kwon H M, Park J W, Roh K B, Lee H, Park B J, Zhang R, Zhang J, Söderhäll K, Ha N C, Lee B L. Molecular control of phenoloxidase-induced melanin synthesis in an insect. The Journal of Biological Chemistry, 2008, 283(37): 25316-25323.

[24] Cerenius L, Babu R, Söderhäll K, Jiravanichpaisal P. In vitro effects on bacterial growth of phenoloxidase reaction products. Journal of Invertebrate Pathology, 2010, 103(1): 21-23.

[25] Huang L H, Christensen B M, Chen C C. Molecular cloning of a second prophenoloxidase cDNA from the mosquito Armigeres subalbatus: prophenoloxidase expression in blood-fed and microfilariae-inoculated mosquitoes. Insect Molecular Biology, 2001, 10(1): 87-95.

[26] 王宝维, 王娜, 葛文华, 岳斌, 张名爱, 史雪萍. 不同硒源对鹅早期生产性能、屠宰性能、肉品质、肌肉常规养分、免疫与抗氧化功能的影响. 中国农业科学, 2011, 44(14): 3016-3026.

Wang B W, Wang N, Ge W H, Yue B, Zhang M A, Shi X P. Effects of different selenium sources on production performance, slaughter performance, meat quality, immune and antioxidant in the early goose. Scientia Agricultura Sinica, 2011, 44(14): 3016-3026. (in Chinese)

[27] 林玉才, 杨颗粒, 武瑞. 硒缺乏对雏鸡免疫器官生长发育的影响. 黑龙江畜牧兽医, 2010(9): 115-116.

Lin Y C, Yang K L, Wu R. Effect of lacking selenium on chickens of the immunologic organs’ growing. Heilongjiang Journal of Animal Science and Veterinary Medicine, 2010(9): 115-116. (in Chinese)

[28] 黎观红, 徐海燕, 许兰姣, 瞿明仁, 游金明, 易中华, 潘珂. 日粮硒添加水平对泰和乌骨鸡生产性能及组织黑色素含量的影响. 中国农业科学, 2011, 44(13): 2777-2786.

Li G H, Xu H Y, XU L J, QU M R, YOU J M, YI Z H, PAN K. Effects of dietary selenium supplementation on growth performance and melanin content in tissues of Taihe silky fowls. Scientia Agricultura Sinica, 2011, 44(13): 2777-2786. (in Chinese)

[29] Cantor A H, Moorehead P D, Musser M A. Comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults. Poultry Science, 1982, 61: 478-484.

[30] Popham H J R, Shelby K S. Effect of inorganic and organic forms of selenium supplementation on development of larval Heliothis virescens. Entomologia Experimentalis et Applicata, 2007, 125(2): 171-178.

[31] Jensen P D, Rivas M D, Trumble J T. Developmental responses of a terrestrial insect detritivore, Megaselia scalaris (Loew) to four selenium species. Ecotoxicology, 2005, 14(3): 313-322.

[32] Hladun K R, Kaftanoglu O, Parker D R, Tran k d, Trumble j t. Effects of selenium on development, survival, and accumulation in the honeybee (Apis mellifera L.). Environmental Toxicology & Chemistry, 2013, 32(11): 2584-2592.

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Appropriate Level of Sodium Selenite for Apis mellifera ligustica Worker Bee Larvae Feed

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