Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (7): 1453-1462.doi: 10.3864/j.issn.0578-1752.2015.07.20

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles    

Appropriate Level of Tryptophan for Apis mellifera Worker Bee Larvae Feed

ZHAO Feng-kui, XU Bao-hua, WANG Hong-fang   

  1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, Shandong
  • Received:2014-10-10 Online:2015-04-01 Published:2015-04-01

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Abstract: 【Objective】 The objective of this research is to study the appropriate levels of tryptophan in Apis mellifera worker bee larvae feed, and to provide a theoretical basis for the nutritional needs of tryptophan. 【Method】 A total of 1 008 one-day-old larvae were randomly divided into seven groups of three replicates of 48 larvae, and were fed different diets with tryptophan at the levels of 7.84, 8.84, 9.84, 10.84, 11.84, 12.84 and 13.84 mg?g-1, respectively. The 5-day-old worker bee larvae were used for measuring TPH (tryptophan hydroxylase) gene and 5-HTR (5-hydroxytryptamine receptors) 1, 2α, 2β, 7 gene expression. The 6-day-old worker bee larvae as samples for determining total protein content, MDA (malondialdehyde), T-AOC (total antioxidant capacity), and the 6-day-old larval hemolymph was taken to be used for measuring the level of free tryptophan. The pupation rate was calculated at 7-day-old. Deposition amount of tryptophan was analyzed using 9-day-old pupa, and the worker bee eclosion rate was calculated at 21-day-old.【Result】Pupation rate and larvae total protein content at 6-day-old, tryptophan deposition at 9-day-old pupa and worker bee eclosion rate at 21-day-old were the highest when the tryptophan level was 10.84 mg?g-1. The expressions of TPH, 5-HT receptors 1, 2α and 2β genes of larvae at 5-day-old and hemolymph free tryptophan content of larvae at 6-day-old were elevated significantly by 10.84-11.84 mg?g-1 tryptophan (P＜0.05). However, 5-HT receptor 7 gene expression of 5-day-old worker bee larvae and MDA level of 6-day-old worker bee larvae fed 12.84-13.84 mg?g-1 tryptophan were higher than worker bee larvae fed 7.84-11.84 mg?g-1 tryptophan (P＜0.05). Dietary supplied with 10.84-13.84 mg?g-1 tryptophan improved significantly 6-day-old worker bee larvae T-AOC compared with dietary with 7.84-9.84 mg?g-1 tryptophan (P＜0.05). 【Conclusion】 The appropriate level of tryptophan for A. mellifera worker bee larvae is 10.84-11.84 mg?g-1.

Key words: Apis mellifera, worker bee, larvae, tryptophan, appropriate level

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