意大利蜜蜂工蜂幼虫饲粮的适宜亚硒酸钠添加水平

doi:10.3864/j.issn.0578-1752.2016.20.018

Abstract

Abstract: 【objective】The objective of this study is to find an appropriate level of sodium selenite which added in the food of Apis mellifera ligustica worker larvae, and to provide a theoretical basis for the nutritional needs of Se.【Method】A total of 1 440 one-day old larvae were randomly allotted into 2 batches, each batch involve 720 larvae, one of the batch was used to measure the pupation rate and eclosion rate, the other was used to measure enzyme activity and molecular index. Two batches were all randomly divided into 6 groups, the control group was fed basic diet and the treatment groups were fed different diets with sodium selenite at the levels of 0.2, 0.4, 0.6, 0.8, and 1.0 mg·kg^-1, respectively. Each group had 5 repetitions and involved 24 larvae in each. The 3-, 5- and 7-day-old larvae were used to measure larvae weight, T-AOC (total antioxidant capacity), T-SOD (total superoxide dismutase), MDA (malondialdehyde), and PO (phenoloxidase). The gene expression of SelD, SerRs, Sbp1 and Sbp2 were measured by using 5-day-old larvae. The pupation rate was calculated at 7-day-old.【Result】Compared with the control group, when the added levels of sodium selenite were 0.2-0.6 mg·kg^-1, the T-AOC was significantly increased (P＜0.05), when the added levels of sodium selenite were 0.2-0.8 mg·kg^-1, the activity of T-SOD was significantly increased (P＜0.05) and the MDA content of all the treatment groups was significantly decreased (P＜0.05). When the added level of sodium selenite was 0.4 mg·kg^-1, the activity of PO was significantly increased (P＜0.05). When the added level of sodium selenite was 0.6 mg·kg^-1, the gene expression levels of SelD, SerRs, Sbp1and Sbp2 was significantly higher than the control in 5-day-old larvae (P＜0.05). When the added level of sodium selenite was 0.4 mg·kg^-1, the weight before pupation was significantly higher than the control (P＜0.05). Compared with the control group, when the added level of sodium selenite was 0.2 mg·kg^-1, the pupation rate were significantly increased (P＜0.05). 【Conclusion】Under artificial condition, when the selenium level of basic diet was 0.21 mg·kg^-1, the appropriate level of sodium selenite which added in the food of A. m. ligusticaby the fitting curve which involve the weight before pupation and the pupation rate, and it means that the appropriate level of selenium which added in the food of A. m. ligustica worker larvae is 0.32-0.36 mg·kg^-1. worker larvae was 0.24-0.33 mg·kg^-1

Key words: Apis mellifera ligustica, worker bee larvae, sodium selenite, appropriate level

SU Zhong-qu, ZHANG Wei-xing, CHI Xue-peng, WANG Hong-fang, XU Bao-hua. Appropriate Level of Sodium Selenite for Apis mellifera ligustica Worker Bee Larvae Feed[J].Scientia Agricultura Sinica, 2016, 49(20): 4047-4055.

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Appropriate Level of Sodium Selenite for Apis mellifera ligustica Worker Bee Larvae Feed

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