Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (9): 1787-1802.doi: 10.3864/j.issn.0578-1752.2016.09.015


• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Identification of Purple Sweet Potato Color of Cultivar Ningzi No. 1 by HPLC-QTOF/MS and Its Effect on Preventing Obesity in High-Fat-Diet-Treated Rats

ZHANG Yi, WANG Hong-yun, NIU Fu-xiang, SUN Jian, XU Fei, ZHU Hong, YUE Rui-xue   

  1. Xuzhou Institute of Agricultural Sciences in Xuhuai District/Sweet Potato Research Institute, Chinese Academy of Agricultural Sciences, Xuzhou 221131, Jiangsu
  • Received:2015-10-30 Online:2016-05-01 Published:2016-05-01

Abstract: 【Objective】Purple sweet potato are the main source for natural antioxidants. This study aims to identify the components of purple sweet potato color of cultivar Ningzi No. 1, and investigate the effectiveness of PSPC for the prevention of obesity and the regulatory mechanism of PSPC in obesity prevention.【Method】 Purple sweet potato color was analyzed using an Agilent 1290 series high-performance liquid chromatography connected to an Accurate-Mass Quadrupole Time-of-Flight/MS (HPLC-QTOF/MS). A pH-differential method was used to determine the total PSPC content. Eight-week-old male Sprague Dawley strain rats were fed on either a normal diet containing 11.4% fat, or a HFD containing 40% fat and intragastrically administered PSPC once a day. Body weights of the rats were measured every week after 6 h of fasting. Activities of serum glucose, triglyceride, and total cholesterol were determined using commercial kits. Serum leptin levels were measured using enzyme-linked immunosorbent assay kits. Expression of leptin and leptin receptor mRNAs in the hypothalamus were detected using Real-time Quantitative PCR. Expression of leptin and its downstream proteins in the hypothalamus were analyzed using Western blotting.【Result】Eleven constituents were separated. Based on previous reports, ten constituents were identified and Peak 1 was newly reported herein. The cyanidin 3-glucoside content of PSPC was 17.4%. After 6 weeks, we first examined the changes in body weight. Our data showed that the effect of middle-dose PSPC (371.6±16.3) g was better than low-dose PSPC (384.0±7.2) g, and the effect of high-dose PSPC (358.2±20.1) g was best. Obesity was prevented and glucose, triglyceride, and total cholesterol induced by the HFD were restored to near-normal levels. Compared with control group rats (2.29±0.28) ng·mL-1, the levels of serum leptin in HFD group rats significantly increased by 65.50%. Compared with HFD group rats (3.79±0.24) ng·mL-1, the levels of serum leptin in low (3.13±0.05) ng·mL-1, middle (2.84±0.12) ng·mL-1, and high-dose (2.64±0.06) ng·mL-1 PSPC group rats were significantly decreased. Both of leptin and leptin receptor mRNAs expression in the hypothalamus was upregulated in the different-dose PSPC group compared with that in the HFD group. High-dose PSPC preserved the leptin signaling capability, leading to a decrease in hypothalamic AMPK α activity.【Conclusion】The anti-obesity effects of high-dose PSPC were mediated via a regulation of leptin/AMPKα signaling in the hypothalamus.

Key words: purple sweet potato, anthocyanidin, HPLC-QTOF/MS, obesity, leptin, AMPK

[1]    Wyatt S B, Winters K P, Dubbert P M. Overweight and obesity: prevalence, consequences, and causes of a growing public health problem. American Journal of the Medical Sciences, 2006, 331: 166-174.
[2]    Yun J W. Possible anti-obesity therapeutics from nature: A review. Phytochemistry, 2010, 71: 1625-1641.
[3]    Harborne J B. Spectral methods of characterizing anthocyanins. Biochemical Journal, 1958, 70: 22-28.
[4]    史光辉, 胡志和, 马科铭, 孙振刚, 武文起, 冯永强. 紫薯花青素提取条件优化及淀粉等产物的制备. 食品科学, 2014, 35: 39-45.
Shi G H, Hu Z H, Ma K M, Sun Z G, Wu W Q, Feng Y Q. Optimization of extraction conditions of anthocyanins from purple sweet potato and simultaneous preparation of starch and other by-products. Food Science, 2014, 35: 39-45. (in Chinese)
[5]    Goda Y, Shimizu T, Kato Y, Nakamura M, Maitani T, Yamada T, Terahara N, Yamaguchi M. Two acylated anthocyanins from purple sweet potato. Phytochemistry, 1997, 44: 183-186.
[6]    Garci-Viguera C, Zafrilla P, Tomas-Barberan F A. The use of acetone as an extraction solvent for anthocaynins from strawberry fruit. Phytochem Analysis, 1998, 9: 274-277.
[7]    Montilla E C, Hillebrand S, Butschbach D, Baldermann S, Watanabe N, Winterhalter P. Preparative isolation of anthocyanins from Japanese purple sweet potato (Ipomoea batatas L.) varieties by high-speed countercurrent chromatography. Journal of Agricultural and Food Chemistry, 2010, 58: 9899-9904.
[8]    Lu J, Wu D M, Zheng Y L, Hu B, Cheng W, Zhang Z F. Purple sweet potato color attenuates domoic acid-induced cognitive deficits by promoting estrogen receptor-α-mediated mitochondrial biogenesis signaling in mice. Free Radical Biology and Medicine, 2012, 52: 646-659.
[9]    罗春丽, 王林, 李杏, 张子程, 张久亮. 紫薯花青素体外抗氧化及对H2O2诱导HepG2细胞氧化损伤的保护作用. 食品科学, 2015, 36: 225-230.
Luo C L, Wang L, Li X, Zhang Z C, Zhang J L. Antioxidant activities and protective effect of anthocyanins from purple sweet potato on HepG2 cell injury induced by H2O2. Food Science, 2015, 36: 225-230. (in Chinese)
[10]   Choi J H, Hwang Y P, Choi C Y, Chung Y C, Jeong H G. Anti-fibrotic effects of the anthocyanins isolated from the purple- fleshed sweet potato on hepatic fibrosis induced by dimethylnitrosamine administration in rats. Food and Chemical Toxicology, 2010, 48: 3137-3143.
[11]   Zhang Z F, Lu J, Zheng Y L, Wu D M, Hu B, Shan Q, Cheng W, Li M Q, Sun Y Y. Purple sweet potato color attenuates hepatic insulin resistance via blocking oxidative stress and endoplasmic reticulum stress in high-fat-diet-treated mice. Journal of Nutritional Biochemistry, 2013, 24: 1008-1018.
[12]   Yoshimot M, Okuno S, Yamaguchi M, Yamakawa O. Antimutagenicity of decylated anthocyanins in purple fleshed sweet potato. Bioscience and Biotechlogy and Biochemistry, 2001, 65: 1652-1655.
[13]   Zhao J G, Yan Q Q, Xue R Y, Zhang J, Zhang Y Q. Isolation and identification of colourless caffeoyl compounds in purple sweet potato by HPLC-DAD-ESI/MS and their antioxidant activities. Food Chemistry, 2014, 161: 22-26.
[14]   Lim S Y, Xu J T, Kim J Y, Chen T Y, Su X, Standard J, Carey E, Griffin J, Herndon B, Katz B, Tomich J, Wang W. Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention. Molecular Nutrition & Food Research, 2013, 57: 1908-1917.
[15]   Liu X L, Mu T H, Sun H N, Zhang M, Chen J W. Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology. Food Chemistry, 2013, 141: 3034-3041.
[16]   Hosseinian F S, Li W D, Beta T. Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chemistry, 2008, 109: 916-924.
[17]   Levin B E, Triscari J, Sullivan A C. Metabolic features of diet-induced obesity without hyperphagia in young rats. American Journal of Physiology-heart and Circulatory Physiology, 1986, 251: 430-440.
[18]   Kim H W, Kim J B, Cho S M, Chung M N, Lee Y M, Chu S M, Che J H, Kim A Y, Cho Y S, Kim J H, Park H J, Lee D J. Anthocyanin changes in the Korean purple-fleshed sweet potato, Shinzami, as affected by steaming and baking. Food Chemistry, 2012, 130: 966-972.
[19]   Shan Q, Zheng Y L, Lu J, Zhang Z F, Wu D M, Fan S H, Hu B, Cai X J, Cai H, Liu P L, Liu F. Purple sweet potato color ameliorates kidney damage via inhibiting oxidative stress mediated NLRP3 inflammasome activation in high fat diet mice. Food and Chemical Toxicology, 2014, 69: 339-346.
[20]   Yoshinaga M, Tanaka M, Nakatani M. Changes in anthocyanin content and composition of developing storage root    of purple-fleshed sweet potato Lam. Breeding Science, 2000, 50: 59-64.
[21]   陆国权, 史锋, 邬建敏, 黄红升, 施志仁. 紫心甘薯花色苷的提取极其组分分析. 天然产物研究与开发, 1997, 3: 48-52.
Lu G Q, Shi F, Wu J M, Huang H S, Shi Z R. Extraction purification and component analysis of anthocyanins from purple sweetpotato. Natural Product Research and Development, 1997, 3: 48-52. (in Chinese)
[22]   Chu H F, Pan M H, Ho C T, Tseng Y H, Wang W W, Chau C F. Variations in the efficacy of resistant maltodextrin on body fat reduction in rats fed different high-fat models. Journal of Agricultural and Food Chemistry, 2014, 62: 192-197.
[23]   Suzuki R, Tanaka M, Takanashi M, Hussain A, Yuan B, Toyoda H, Kuroda M. Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway. Nutrition & Metabolism, 2011. doi: 10.1186/1743-7075-8-14: 8-14.
[24]   Suda I, Oki T, Masuda M, Nishiba Y, Furuta S, Matsugano K, Sugita K, Terahara N. Direct absorption of acylated anthocyanin in purple-fleshed sweet potato into rats. Journal of Agricultural and Food Chemistry, 2002, 50: 1672-1676.
[25]   Milbury P E, Kalt W. Xenobiotic metabolism and berry flavonoid transport across the blood-brain barrier. Journal of Agricultural and Food Chemistry, 2010, 58: 3950-3956.
[26]   Tan G D, Fielding B A, Currie J M, Humphreys S M, Désage M, Frayn K N, Laville M, Vidal H, Karpe F. The effects of rosiglitazone on fatty acid and triglyceride metabolism in type 2 diabetes. Diabetologia, 2005, 48: 83-95.
[27]   张毅, 娄大雷, 关诗扬, 蔡翔君, 周军. 瘦素水平与代谢综合症的关系. 现代生物医学进展, 2012, 12: 6795-6797.
Zhang Y, Lou D L, Guan S Y, Cai X J, Zhou J. A relationship between leptin level and metabolism syndrome. Progress in Modern Biomedicine, 2012, 12: 6795-6797. (in Chinese)
[28]   Zhang Y, Proenca R, Maffei M , Barone M, Leopold L, Friedman J M. Positional cloning of the mouse obese gene and its human homologue. Nature, 1994, 372: 425-432.
[29]   Friedman J M, Halaas J L. Leptin and the regulation of body weight in mammals. Nature, 1998, 395: 763-770.
[30] Minokoshi Y, Kim Y B, Peroni O D, Fryer L G, Müller C, Carling D, Kahn B B. Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase. Nature, 2002, 415: 339-343.
[31]   Baumann H, Morella K K, White D W, Dembski M, Bailon P S, Kim H, Lai C F, Tartaglia L A. The full-length leptin receptor has signaling capabilities of interleukin 6-type cytokine receptors. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93: 8374-8378.
[32]   Minokoshi Y, Alquier T, Furukawa N, Kim Y B, Lee A, Xue B, Mu J, Foufelle F, Ferré P, Birnbaum M J, Stuck B J, Kahn B B. AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature, 2004, 428: 569-574.
[33]   Hill J W, Williams K W, Ye C, Luo J, Balthasar N, Coppari R, Cowley M A, Cantley L C, Lowell B B, Elmquist J K. Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice. Journal of Clinical Investigation, 2008, 118: 1796-1805.
[34]   Hardie D G, Scott J W, Pan D A, Hudson E R. Management of cellular energy by the AMP-activated protein kinase system. FEBS Letters, 2003, 546: 113-120.
[35]   Dagon Y, Hur E, Zheng B, Wellenstein K, Cantley L C, Kahn B B. p70S6 kinase phosphorylates AMPK on serine 491 to mediate leptin's effect on food intake. Cell Metabolism, 2012, 16: 104-112.
[1] LAN Qun,XIE YingYu,CAO JiaCheng,XUE LiE,CHEN DeJun,RAO YongYong,LIN RuiYi,FANG ShaoMing,XIAO TianFang. Effect and Mechanism of Caffeic Acid Phenethyl Ester Alleviates Oxidative Stress in Liquid Preservation of Boar Semen Via the AMPK/FOXO3a Signaling Pathway [J]. Scientia Agricultura Sinica, 2022, 55(14): 2850-2861.
[2] HaiXia ZHENG,YuLin GAO,FangMei ZHANG,ChaoXia YANG,Jian JIANG,Xun ZHU,YunHui ZHANG,XiangRui LI. Cloning of Heat Shock Protein Gene Ld-hsp70 in Leptinotarsa decemlineata and Its Expression Characteristics under Temperature Stress [J]. Scientia Agricultura Sinica, 2021, 54(6): 1163-1175.
[3] SHI Xin,LI Sha,WANG ZhiMin,FU KaiYun,FU WenJun,JIANG WeiHua. Resistance Monitoring to Thiamethoxam and Expression Analysis of Cytochrome P450 Genes in Leptinotarsa decemlineata from Xinjiang [J]. Scientia Agricultura Sinica, 2021, 54(14): 3004-3016.
[4] MA Jian, LI CongCong, HUANG YaTing, XIE YuLi, CHENG LingLing, WANG JianShe. Fine Mapping and Candidate Gene Analysis of Seed Coat Color Gene CmSC1 in Melon [J]. Scientia Agricultura Sinica, 2021, 54(10): 2167-2178.
[5] HAO XiaoYan,MU ChunTang,QIAO Dong,ZHANG XuanZi,YANG WenJun,ZHAO JunXing,ZHANG ChunXiang,ZHANG JianXin. Effects of High-Concentrate Diet Supplemented with Grape Seed Proanthocyanidins on Rumen fermentation, Inflammatory and Antioxidant Indicators of Rumen and Serum in Lambs [J]. Scientia Agricultura Sinica, 2021, 54(10): 2239-2248.
[6] CHANG XueLing, ZHANG ZongWen, LI YanQin, GAO Jia. Cloning and Expression Analyses of the mate Gene in Buckwheat [J]. Scientia Agricultura Sinica, 2018, 51(11): 2038-2048.
[7] LU Hui-hui, LIN Zhi-qiang, TAN Wan-zhong, LUO Hua-dong, XIAN Fei, BI Chao-wei, YU Yang, YANG Yu-heng. Insecticidal Protein Genes of Bacillus thuringiensis Strain CPB012 and Its Effects in Controlling Different Insect Pests [J]. Scientia Agricultura Sinica, 2015, 48(6): 1112-1121.
[8] LI Wen-Feng, XIAO Xu-Lin. Effect of Different Drying Methods on Drying Efficiency and Quality of Purple Sweet Potato [J]. Scientia Agricultura Sinica, 2014, 47(7): 1397-1408.
[9] LI Chao, CHENG Deng-Fa, LIU Huai, ZHANG Yun-Hui, SUN Jing-Rui. Effects of Temperature on the Distribution of the Colorado Potato Beetle (Leptinotarsa decemlineata)—Effect of High Temperature on Its Emergence in Turpan, Xinjiang [J]. Scientia Agricultura Sinica, 2013, 46(4): 737-744.
[10] LI Wen-Feng, XIAO Xu-Lin, WANG Wei. Drying Characteristics and Model of Purple Sweet Potato in Air-impingement Jet Dryer [J]. Scientia Agricultura Sinica, 2013, 46(2): 356-366.
[11] XIA Cheng,WANG Zhe,ZHANG Hong-you,XU Chuang,ZHANG Cai
. Characters of Metabolism, Endocrine and Tissues Genes Expression of Dairy Cows Affected by Fatty Liver#br# [J]. Scientia Agricultura Sinica, 2010, 43(8): 1696-1702 .
[12] TAO Yong-sheng,ZHANG Li. Correlation Analysis of CIELab Parameters and Anthocyanidins of Different Red Wines
[J]. Scientia Agricultura Sinica, 2010, 43(20): 4271-4277 .
[13] . Fusion Expression of Duck Leptin Gene in Escherichia coli and Determination of Recombinant Protein Bioactivity [J]. Scientia Agricultura Sinica, 2007, 40(11): 2615-2620 .
Full text



No Suggested Reading articles found!