苹果bZIP转录因子家族生物信息学分析及其在休眠芽中的表达

doi:10.3864/j.issn.0578-1752.2016.07.010

Abstract

Abstract: 【Objective】In this study, putative bZIP transcription factor-encoding genes in the apple (Malus×domestica Borkh.) genome were identified, so as to provide a basis for studying the theoretical roles of bZIP genes in the bud dormancy, and to provide valuable information for bZIP genes in apple. 【Method】 The Hidden Markov Model profiles of the bZIP domains (PF00170 and PF07716) downloaded from Pfam were used to search the database using HMMER, ver. 3.0 with the default E-value. The obtained amino acid sequences were analyzed with the bioinformatics softwares, including Clustal Omega, MEGA6.0, MapInspect, DNAMAN 6.0 and MEME4.10.2. Furthermore, Microarray analysis and qRT-PCR results indicated that many MdbZIP genes may be involved in regulating bud dormancy in different cultivar of apples. 【Result】 In Total 120 MdbZIP genes were found in the apple genome. Phylogenetic analyses of the genes based on A. thaliana counterparts indicated that the transcription factors can be categorized into 10 different groups (Groups A–I and S), Chromosome mapping analysis showed that 109 MdbZIP genes were distributed unevenly on 17 chromosomes. Except for 11 genes that were not located on chromosomes, the largest number of MdbZIP genes were found on chromosomes 8 (thirteen genes), only 1 genes located on chromosome 1, some chromosomal regions had a relatively high density of MdbZIP genes. The results of gene structure analysis revealed that MdbZIP gene contained 0-23 exons, 23 bZIP genes were intronless and were found in Groups S (nine genes) and F (one gene), bZIP gene structure were highly conserved in apples. Conserved motif analysis showed that the conserved motifs 1, which specify the bZIP domain were observed in all apple bZIP proteins, motif 10 and 14 as the known domain were observed in Group D and G, respectively. Furthermore, microarray data indicated that many MdbZIP genes may be involved in regulating bud dormancy release. And qRT-PCR results indicated that ABA induce eight members of group A expression in high-chill and low-chill apples, but the largest number of differentially expressed genes was found in group D with cold temperatures. 【Conclusion】 These results suggested that MdbZIP gene family was highly and structurally conserved, and involved in abscisic acid (ABA) signaling and cold stresses thereby possibly involved into the regulation of bud dormancy in apples.

Key words: apple (Malus×domestica Borkh.) , bZIP transcription factor, bud dormancy, phylogenetic analysis, sequence alignment, microarray analysis, qRT-PCR

SUN Ming-yue, ZHOU Jun, TAN Qiu-ping, FU Xi-ling, CHEN Xiu-de, LI Ling, GAO Dong-sheng. Analysis of Basic Leucine Zipper Genes and Their Expression During Bud Dormancy in Apple (Malus×domestica)[J].Scientia Agricultura Sinica, 2016, 49(7): 1325-1345.

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Analysis of Basic Leucine Zipper Genes and Their Expression During Bud Dormancy in Apple (Malus×domestica)

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