Abstract: 【Objective】 The aim of the experiment is to explore the relationship between tapetum degradation and nutrient metabolism in anther and pollen abortion in CMS of wheat. 【Method】 Wheat S-1376A CMS [(S)-1376 (A) ] and its maintainer line [(A)-1376 (B) ] were used as test materials. The development condition of the nucleus of pollens at tricellular was surveyed through dyed by DAPI, and stained by KI-I2 to observe the starch accumulated in pollens at tricellular. Semi-thin sectioning was employed to observe and compare tapetum development process, polysaccharides, lipids and proteins accumulation in the sterile line and maintainer line. The cell Sene Entry was utilized to collect images and to calculate the area of the microspores and the tapetum cells in the section. IPP 6.0 was adopted to analyze the mean optical density and IOD at each stage in 1376 and (S)-1376. 【Result】 The data demonstrated that in (S)-1376, compared with 1376, the tapetum cells in advance degradated at the tetrad stage, and the pollen nucleus development was retarded, the majority of which merely developed the late uninucleate. Pollens were dyed yellow by KI-I2 at tricellular in (S)-1376, which certified there was no starch present in the pollens result in them thorough abortion. Area of the microspores and the tapetum cells in the section was extremely huge at the tetrad in (S)-1376. In (S)-1376, compared with 1376, a great number of starches and proteins accumulated in microspores at the tetrad stage, but from late uninucleate stage this situation undergone dramatic difference that the starches and proteins significantly decreased; whereas the lipids were significantly poorer in each period. In (S)-1376, polysaccharides and proteins were significantly higher than 1376 in the tapetum in tetrad, however, polysaccharides were less in early uninucleate and proteins were low in late uninucleate, respectively. Notably, lipids were lower in each period in the tapetum in (S)-1376. The contents of polysaccharides and proteins were greater at tetrad stage in (S)-1376, while at the late uninucleate the polysaccharides, lipids and proteins were lower in (S)-1376 than that in 1376 ones. At bicellular and tricellular of (S)-1376, the substances of the cytoplasm were no longer increased, meanwhile starches and lipids stopped growing, too, the big vacuoles of the vegetative cell were still present and the pollens became malformation at last. 【Conclusion】 Tapetum of (S)-1376 degraded more earlier than (A)-1376 that caused nutrient metabolism abnormal, leading to the supplying of the polysaccharides, lipids and proteins were aberrant from the late development stage of uninucleate to binucleate, and then the nuclear of pollens fission was abnormal, ultimately, the pollens of (S)-1376 were aborted directly.

Key words: wheat , tapetum , polysaccharides , lipids , proteins , cytoplasmic male sterility