Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4344-4353.doi: 10.3864/j.issn.0578-1752.2013.20.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Comparative Studies on the Bacteriostatic Activity of the Three Bacillus Strains and Its Effects on Air Microorganisms in Broiler House

 TONG  Yong-Juan-1, SA  Ren-Na-1, ZHANG  Hong-Fu-1, SUN  Chun-Yang-1, XING  Huan-1, PENG  Yi-Zhu-1, WEI  Zhong-Hua-2, WANG  Xue-Jing-2   

  1. 1.Institute of Animal Science,Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Nutrition,Beijing 100193
    2.The Animal Husbandry and Veterinary Institute of Heibei，Baoding 071000, Hebei
  • Received:2013-04-08 Online:2013-10-15 Published:2013-06-14

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Abstract: 【Objective】The present study was conducted to evaluate the biological characteristics of 2 strains of Bacillus amyloliquefaciens and 1 strain of Bacillus thuringiensis, and the bacteriostatic activity of these three bacillus strains against Escherichia coli. After being sprayed the mixture of three bacillus suspension to the broiler house, the number of E.coli and staphylococcus aureus in the air was detected in order to evaluate the effect of bacillus on improvment of environment.【Method】In this study, the colony morphology and the growth curve of the three bacillus were examined. All of the three bacteria were examined for their antimicrobial activity against E. coli (CVCC1570) by using the agar diffusion system. The optimum combination level of the three bacteria in the mixed product was investigated by using the orthogonal design in three factors and three levels. The mixed product of the three bacillus strains was sprayed in the poultry house. Three houses were selected, one for blank control(A), one for chemical disinfectant (benzalkonium chloride) as positive control(B), and one for bacillus treatment(C). Each treatment contained three replications. The numbers of E.coli and Staphylococcus aureus in the air of the poultry house were detected every week.【Result】The results showed that all strains appeared as Gram-positive and aerobic bacillus. Bacillus SKN01, Bacillus SKN02 had the similar growth curve entering the logarithmic phase in the first two hours. Bacillus SKN03 grew slowly and spent long time to enter the logarithmic phase. The effective bacteriostatic activity concentration of single bacillus strain against E. coli (CVCC1570) was 108 CFU•mL-1. The most effective concentration of antimicrobial activity in the mixture of three bacillus strains was 4×108CFU•mL-1, 4×108CFU•mL-1 and 6×108CFU•mL-1. Spraying the mixture of bacillus suspension in the broiler house could effectively reduce the number of E.coli and S. aureus in the air, especially at the later growth stage of chickens.【Conclusion】The bacillus strain had antimicrobial activity against E.coli. Spraying the mixture of the three bacillus suspension in the broiler house could reduce the number of E.coli and S. aureus in the air and could improve the environment effectively.

Key words: Bacillus , Bacteriostatic activity , Escherichia coli , Staphylococcus aureus

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