磷脂酰肌醇3-磷酸对UV-B诱导蚕豆保卫细胞中过氧化氢产生和气孔关闭的影响

doi:10.3864/j.issn.0578-1752.2013.20.007

Abstract

Abstract: 【Objective】 The role of phosphatidylinositol 3-phosphate (PI3P), the product of phosphatidylinositol 3-kinase (PI3K), in UV-B-induced hydrogen peroxide (H2O2) generation in guard cells and stomatal closure was analyzed to provide valuable evidence for further elucidating the mechanism of UV-B signaling transduction in plants. 【Method】 By using PI3K inhibitors wortmannin (WM) and LY294002 (LY) to inhibit the production of PI3P, and by using diphenylene iodonium (DPI) and salicylhydroxamic acid (SHAM) to inhibit H2O2 production via NADPH oxidase pathway and peroxidase pathway respectively, the role of PI3P in 0.8 W?m-2 UV-B-induced H2O2 production in guard cells and subsequent stomatal closure was studied via stomatal bioassay and laser-scanning confocal microscopy in epidermal strips of broad bean (Vicia faba L.). 【Result】Both the UV-B-induced H2O2 production in guard cells and stomatal closure were significantly inhibited by WM and LY. Exogenous H2O2 rescued the inhibitory effect of WM and LY on UV-B-induced stomatal closure, but exogenous H2O2-induced stomatal closure was not inhibited by WM and LY. Both the UV-B-induced H2O2 production in guard cells and stomatal closure were significantly inhibited by the reactive oxygen species scavenger and SHAM, but not inhibited by DPI. 【Conclusion】 PI3P mediates UV-B-induced stomatal closure via activating the peroxidase-dependent H2O2 production in guard cells of broad bean.

Key words: phosphatidylinositol 3-phosphate , broad bean , hydrogen peroxide , UV-B radiation , stomatal movement

LI Hui-Min-12, HU Jie-1, HE Jun-Min-1. Effect of Phosphatidylinositol 3-Phosphate on UV-B-Induced H2O2 Production in Guard Cells and Stomatal Closure of Broad Bean[J].Scientia Agricultura Sinica, 2013, 46(20): 4246-4253.

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Effect of Phosphatidylinositol 3-Phosphate on UV-B-Induced H2O2 Production in Guard Cells and Stomatal Closure of Broad Bean

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