保卫细胞微管骨架参与蛋白丝氨酸/苏氨酸磷酸化调节的气孔运动

doi:10.3864/j.issn.0578-1752.2012.21.003

Abstract

Abstract: 【Objective】 The interaction between microtubules (MTs) and protein serine/threonine phosphorylation in guard cells was investigated in order to provide a reliable evidence for better understanding of the mechanism of stomatal movment. 【Method】Pharmacological analysis, confocal laser scanning microscopy, and western blotting were used to explore the possible interaction of these two elements. 【Result】 In vivo stomatal aperture assays revealed that staurosporine (STS), one well-known specific inhibitor of serine/threonine protein kinase, enhanced the light-induced stomatal opening. While, okadaic acid (OA) and calyculin A (CalA), another two inhibitors of 1/2A phosphatase, showed the opposite effects under the same condition. In addition, it was found that these changes of stomatal movement could be reversed or became intensified to some extent when microtubule-specific drug, taxol or vinblastine was present during the treatments above. Furthermore, OA and CalA also had significant influences on the MT organization in the opening guard cells. In the presence of OA or CalA, the fine radial MT arrays tended to be broken down or rearranged into random network. And guard cells kept in STS showed increase in radial MT arrays and stomatal aperture. MT-targeted drugs, taxol and vinblastine, also altered the effects of three inhibitors (OA, CalA, STS) on MT organization partiallly in a similar manner. Immunoblot detection of total extract from guard cells confirmed that tubulin-subunits were phosphorylated on serine. 【Conclusion】 The results suggest that reversible protein serine/threonine phosphorylation may regulate the MT dynamics of guard cells during stomatal movement.

Key words: microtubule, protein serine/threonine phosphorylation, stomatal movement

YE Zi, HUANG Cong-Cong, YU Rong. Microtubules in Guard Cells Involve in Serine/Threonine Phosphorylation During Stomatal Movement[J].Scientia Agricultura Sinica, 2012, 45(21): 4351-4360.

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Microtubules in Guard Cells Involve in Serine/Threonine Phosphorylation During Stomatal Movement

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