填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度

doi:10.3864/j.issn.0578-1752.2012.10.013

Abstract

Abstract: 【Objective】An experiment was conducted to investigate different fatty acids deposition, FAS mRNA expression abundance and their correlation in goose liver tissue in different overfeeding periods. 【Method】 Two hundred Qingnong gray geese at the age of 100 days were selected and overfed. Ten geese were randomly selected and slaughtered every 6 days. The weight of liver, EE, the content of fatty acids and FAS mRNA expression abundance were measured. 【Result】 The weight and the fat content of liver increased with the extension of overfeeding time. They arrived to the peak during 18-24 d (504.67 g/6 d) and 12-18 d secondly. The content of SFA and MUFA in liver increased with the extension of overfeeding time, especially during 12-18 d and 18-24 d, however, PUFA deposited mainly in the late period (18-30 d). Overall, in the whole process of overfeeding, fatty acids in the late period deposited significantly (P＜0.05) or highly significantly (P＜0.01) more than the early stage. Every 100 g liver tissue has 0.6028 g myristic acid (C14﹕0), 17.72 g palmitic acid (C16﹕0), 7.25 g octadecanoic acid (C18﹕0), 2.75 g palmitoleic acid (C16﹕1), 37.42 g oleic acid (C18﹕1), 0.3078 g dodecenoicacid (C20﹕1), 0.43 g linoleic acid (C18﹕2) which were mainly deposited after overfeeding 30 days. FAS mRNA expression abundance increased to the peak at the overfeeding 24th day, then decreased quickly. During overfeeding 0-24 d, FAS mRNA expression abundance in goose liver tissue had a highly significant positive correlation with the fatty liver weight, EE, SFA and MUFA (P＜0.01), and a negative correlation with PUFA although it was not significant (P＞0.05). At the overfeeding 30th day there was no significant correlation between them (P＞0.05).【Conclusion】 The research showed that the content of EE, SFA and MUFA in liver increased with the overfeeding time and the fatty liver weight. The deposition velocity was the fastest in the period of overfeeding 18-24 d. In mRNA expression, FAS gene regulated the deposition velocity of the fat of liver tissue in fatty liver goose. It increased rapidly in the early and middle overfeeding period, however decreased in the late period.

Key words: overfeeding goose, liver, fatty acids, FAS gene

SHU Chang-Ping, WANG Bao-Wei, LI Zhen, GE Wen-Hua, ZHANG Ming-Ai, YUE Bin. Fatty Acids Deposition and FAS mRNA Expression Abundance in Liver Tissue of Overfeeding Goose[J].Scientia Agricultura Sinica, 2012, 45(10): 2002-2011.

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References

[1]王程. 朗德鹅和溆浦鹅产肝性能与脂肪沉积规律的比较研究[D]. 武汉: 华中农业大学, 2006.

Wang C. Comparative study between Landes goose and Xupu goose on the regularity of the fatty liver performance and fat storage[D]. Wuhan: Huazhong Agricultural University, 2006. (in Chinese)

[2]苏胜彦, 李齐发, 陈睿, 曾怡, 祝红生, 张翔, 王艳平, 谢庄, 吴松青, 刘玉弟. 填饲对朗德鹅产肝性能、肝脏组织学和脂生成基因表达水平的影响. 中国农业科学, 2009, 42(7): 2523-2530.

Su S Y, Li Q F, Chen R, Zeng Y, Zhu H S, Zhang X, Wang Y P, Xie Z, Wu S Q, Liu Y D. Measurement of liver performance, histology and adipogenic genes transcriptional expression of Landes goose liver in response to overfeeding. Scientia Agricultura Sinica, 2009, 42(7): 2523-2530. (in Chinese)

[3]刘振春, 段绪, 李慧, 李侠, 吴伟. 鹅肥肝中脂肪酸沉积规律研究. 食品科学, 2010, 31(23): 49-51.

Liu Z C, Duan X, Li H, Li X, Wu W. Deposition regularity of fatty acids in livers of fat geese. Food Science, 2010, 31(23): 49-51. (in Chinese)

[4]Molette C, Berzaghi C, Zotte AD, Remignon H, Babile R. The use of near-infrared reflectance spectroscopy in the prediction of the chemical composition of goose fatty liver. Poultry Science, 2001, 80(11): 1625-1629.

[5]Mourot J, Guy G, Lagarrigue S, Peiniau P, Hermier D. Role of hepatic lipogenesis in the susceptibility to fatty liver in the goose (Anser anser). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2000, 126(1): 81-88.

[6]王继文, 韩春春, 李亮, 许恒勇, 叶建强, 蒋立, 卓伟华. 填饲对鹅肝脂质沉积及相关基因表达的影响. 中国家禽, 2008, 30(20): 14-17.

Wang J W, Han C C, Li L, Xu H Y, Ye J Q, Jiang L, Zhuo W H. Effect of overfeeding on lipids deposition and gene expression of related genes in goose liver. China Poultry, 2008, 30(20): 14-17. (in Chinese)

[7]韩春春, 黄晓宇, 王继文. 鹅LXRα基因的克隆及填饲对其mRNA水平的影响. 畜牧兽医学报, 2009, 40(9): 1405-1409.

Han C C, Huang X Y, Wang J W. Cloning of goose LXRα gene and the effect of overfeeding on its mRNA level. Acta Veterinaria et Zootechnica Sinica, 2009, 40(9): 1405-1409. (in Chinese)

[8]Zhao A Y, Wang X D, Chen G H, Lu L Z. Low-level expression of cholesterol 7 α-hydroxylase is associated with the formation of goose fatty liver. Poultry Science, 2011, 90(5): 1045-1049.

[9]潘志雄, 王继文, 唐慧, 向梳瑕, 王静, 吕佳, 杨超, 韩春春. 鹅Perilipin基因部分片段的克隆、不同品种及填饲对组织mRNA表达水平的影响. 畜牧兽医学报, 2010, 41(8): 939-943.

Pan Z X, Wang J W, Tang H, Xiang S X, Wang J, Lü J, Yang C, Han C C. Cloning of goose Perilipin gene, tissues expression and the effect of overfeeding on its mRNA level. Acta Veterinaria et Zootechnica Sinica, 2010, 41(8): 939-943. (in Chinese)

[10]Zhao A Y, Tang H C, Lu S F, He R G. Identification of a differentially-expressed gene in fatty liver of overfeeding geese. Acta Biochimica Biophysica Sinica, 2007, 39(9): 649-656.

[11]Sourdioux M, Douaire M, Delabrosse Y. DNA polymorphisms of lipogenesis genes and analysis of linkage with fatness in turkeys. Poultry Science, 1996, 75: 1018-1026.

[12]刘作华. 日粮能量水平对猪肌内脂肪沉积的影响及作用机制研究[D]. 雅安: 四川农业大学, 2008.

Liu Z H. Effete of dietary energy density on intramuscular fat deposition for pigs and its underlying mechanism[D]. Ya’an: Sichuan Agricultural University, 2008. (in Chinese)

[13]中华人民共和国卫生部. 中国国家标准化管理委员会. 食品中脂肪的测定(GB/T 5009.6—2003), 2003.

Ministry of Public Health of the People’s Republic of China. Standardization Administration of the People’s Republic of China. Determination of fat in foods (GB/T 5009.6-2003), 2003. (in Chinese)

[14]中华人民共和国国家质量监督检验检疫总局. 中国国家标准化管理委员会. 动植物油脂脂肪酸甲酯制备(GB/T 17376—2008), 2008.

General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (AQSIQ). Standardization Administration of the People’s Republic of China. Animal and vegetable fats and oils-preparation of methyl esters of fatty acids (GB/T 17376-2008), 2008. (in Chinese)

[15]中华人民共和国国家质量监督检验检疫总局. 中国国家标准化管理委员会. 动植物油脂脂肪酸甲酯的气相色谱分析(GB/T 17377—2008), 2008.

General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (AQSIQ). Standardization Administration of the People’s Republic of China. Animal and vegetable fats and oils-analysis by gas chromatography of methyl esters of fatty acids (GB/T 17377-2008), 2008. (in Chinese)

[16]Hermier D, Saadoun A, Salichon M R, Sellier N, Daniel R P, John C M. Plasma lipoproteins and liver lipids in two breeds of geese with different susceptibility to hepatic steatosis: Changes induced by development and force-feeding. Lipids, 1991, 26(5): 331-339.

[17]Fournier E, Peresson R, Guy G, Hermier D. Relationships between storage and secretion of hepatic lipids in two breeds of geese with different susceptibility to liver steatosis. Poultry Science, 1997, 76(4): 599-607.

[18]Hermier D, Rousselot-Pailley D, Peresson R, Sellier N. Influence of orotic acid and estrogen on hepatic lipid storage and secretion in the goose susceptible to liver steatosis. Biochimica et Biophysica Acta, 1994, 1211(1): 97-106.

[19]李勇, 蔡辉益, 刘国华, 杨志刚, 常文环, 张姝. 能量和蛋白质水平对1—21日龄肉仔鸡生长性能、体蛋白质及体脂肪沉积的影响. 动物营养学报, 2010, 22(4): 886-893.

Li Y, Cai H Y, Liu G H, Yang Z G, Chang W H, Zhang S. Effects of dietary energy and protein levels on the growth performance, body protein and body fat deposition of broilers aged from 1 to 21 days. Chinese Journal of Animal Nutrition, 2010, 22(4): 886-893. (in Chinese)

[20]李勇, 蔡辉益, 刘国华, 杨志刚, 常文环, 张姝. 0—3 及 4—6 周龄肉仔鸡日粮能量及蛋白质沉积效率参数研究. 中国农业科学, 2010, 43(17): 3624-3632.

Li Y, Cai H Y, Liu G H, Yang Z G, Chang W H, Zhang S. Study on the parameters of efficiencies of energy and protein deposition in broilers between 0-3 and 4-6 weeks. Scientia Agricultura Sinica, 2010, 43(17): 3624-3632. (in Chinese)

[21]曹华斌, 胡国良, 郭小权, 张彩英, 李浩棠. 高能低蛋白日粮饲养蛋鸡肝脏脂肪沉积及其组织病理学观察. 中国兽医学报, 2010, 30(7): 1009-1012.

Cao H B, Hu G L, Guo X Q, Zhang C Y, Li H T. Fat accumulation and histopathology of liver from laying hens fed with high-energy- low-protein diet. Chinese Journal of Veterinary Science, 2010, 30(7): 1009-1012. (in Chinese)

[22]孙云子. 不同能量饲料对朗德鹅产肝性能影响的研究[D]. 武汉: 华中农业大学, 2004.

Sun Y Z. Effect of the different energy feeding on the fatty liver performance of Landaise geese[D]. Wuhan: Huazhong Agricultural University, 2006: 15-32. (in Chinese)

[23]范永存, 王宝维, 于世浩, 王巧莉, 张倩, 孙鹏, 王娜, 姜晓霞. 不同脂肪源对鹅产肝性能和品质影响的研究. 中国家禽, 2008, 30(24): 14-17.

Fan Y C, Wang B W, Yu S H, Wang Q L, Zhang Q, Sun P, Wang N, Jiang X X. Effect of different fatty sources on production performance and quality of fatty liver in goose. China Poultry, 2008, 30(24): 14-17. (in Chinese)

[24]佘德勇, 詹凯, 胡胜强, 郝金平. 北京鸭和樱桃谷鸭填饲条件下脂肪沉积性能比较. 中国家禽, 2007, 29(2): 15-17.

She D Y, Zhan K, Hu S Q, Hao J P. Comparison of fat deposition traits between force-feeding Peking ducks and Cherry Valley ducks. China Poultry, 2007, 29(2): 15-17. (in Chinese)

[25]Chandi C M, Triparna G C, Toshi Y, Goutam G C, Nandini G C. Fish oil prevents breast cancer cell metastasis to bone. Biochemical and Biophysical Research Communications, 2010, 402(4): 602-607.

[26]Smith S, Witkowski A, Joshi A K. Structural and functional organization of the animal fatty acid synthase. Progress in Lipid Research, 2003, 42(4): 289-317.

[27]Semenkovich C F. Regulation of fatty acid synthase (FAS). Progress in Lipid Research, 1997, 36(1): 43-53.

[28]Denechaud P D, Dentin R, Girard J, Postic C. Role of ChREBP in hepatic steatosis and insulin resistance. FEBS Letters, 2008, 582(1): 68-73.

[29]Loftus T M., Jaworsky D E, Frehywot G L, Townsend C A, Ronnett G V, Lane M D, Kuhajda F P. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science, 2000, 288(5475): 2379-2381.

[30]熊文中, 杨凤, 周安国. 猪重组生长激素对不同杂交育肥猪脂肪代谢调控的研究. 畜牧兽医学报, 2001, 32(1): 1-4.

Xiong W Z, Yang F, Zhou A G. Study of regulation of exogenous recombinant somatotropin on fat metabolism in different cross-finishing pigs. Acta Veterinaria et Zootechnica Sinica, 2001, 32(1): 1-4. (in Chinese)

[31]田维熙, 董妍, 权晖, 陈文峰. 不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系. 生物化学杂志, 1996, 12(2): 234-236.

Tian W X, Dong Y, Quan H, Chen W F. The dependence of fat level of hen on activity of fatty acid synthase in liver on different ages. Chinese Biochemical Journal, 1996, 12(2): 234-236. (in Chinese)

[32]Kim T S, Freake H C. High carbohydrate diet and starvation regulate lipogenic mRNA in rats in a tissue-specific manner. The Journal of Nutrition, 1996, 126: 611-617.

[33]Han C C, Wang J W, Li L, Zhang Z X, Wang L, Pan Z X. The role of insulin and glucose in goose primary hepatocyte triglyceride accumulation. The Journal of Experimental Biology, 2009, 212: 1553-1558.

[34]彭祥伟, 范守城, 邢豫川, 谢友慧, 王阳铭, 黄勇富, 程必刚, 张昌莲. 鹅肥肝的生产及其FAS活性测定. 中国家禽学报, 2005, 9(1): 48-51.

Peng X W, Fan S C, Xing Y C, Xie Y H, Wang Y M, Huang Y F, Cheng B G, Zhang C L. Production of geese fatty liver and its FAS activity for mensurating. Chinese Poultry Science, 2005, 9(1): 48-51. (in Chinese)

[35]张磊, 吴伟, 高光, 闫文英, 张镇, 林海涛. 脂肪酸合成酶(FAS)的SNP与鹅肥肝性能关联研究. 吉林农业大学学报, 2009, 31(1): 77-80.

Zhang L, Wu W, Gao G, Yan W Y, Zhang Z, Lin H T. The single nucleotide polymorphism of FAS gene associated with fatty liver traits in geese. Journal of Jilin Agricultural University, 2009, 31(1): 77-80. (in Chinese)

[36]Zhu L H, Meng H, Duan X J, Xu G Q, Zhang J, Gong D Q. Gene expression profile in the liver tissue of geese after overfeeding. Poultry Science, 2011, 90(1): 107-117.

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