Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1521-1531.doi: 10.3864/j.issn.0578-1752.2012.08.008

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effect of P Deficiency on the Emergence of Astragalus L. Root Exudates and Mobilization of Sparingly Soluble Phosphorus

 LAN  Zhong-Ming, LIN  Xin-Jian, ZHANG  Wei-Guang, ZHANG  Hui, WU  Yi-Qun   

  1. 福建省农业科学院土壤肥料研究所/福建省农业科学院农业资源与环境中心，福州 350013
  • Received:2011-07-21 Online:2012-04-15 Published:2011-08-31

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Abstract: 【Objective】 The objective of this study was to investigate the mobilization and uptake capacity of sparingly soluble phosphorus by root exudate of Astragalus L., and genotypic variation of different Astragalus L. in activation of sparingly soluble phosphorus. 【Method】 Hoagland nutrient solution culture was carried out to collect root exudate and test the mobilization of sparingly soluble phosphorus via concentrated vacuum rotary evaporator, and the content of organic acids was analyzed by high performance liquid chromatography (HPLC). 【Result】 Under the condition of P-deficiency stress, root radius from different genotypes of Astragalus L. reduced, while the root-shoot ratio and root surface area were significantly higher than P-supply. Root exudates were oxalic acid, tartaric acid, citric acid, malic acid and so on, but it mainly exuded oxalic acid. Under the condition of P-deficiency, secreation of oxalic was significantly different among variou genotypes, and the amout of organic acid secretion was significantly higher than P-supply. Minzi 1, Zhezi 5 and Minzi 6 in organic acid secretion were significantly higher than Yijiangzi, Yujiangdaye. Root exudates on insoluble Al-P and Fe-P had certain activation capacity under P-deficiency and P-supply. The activation P values for Al-P and Fe-P were 36.40-157.39 μg•g-1, 32.20-139.42 μg•g-1, and P-deficient root exudates on sparingly soluble phosphate was higher than P-supply, and activation capacity of Al-P was slightly higher than Fe-P. Through the activation of simulation experiment, it was confirmed that organic acids could mobilize sparingly soluble Al-P and Fe-P, and the difference was significant.【Conclusion】 Under P-deficiency stress, Astragalus L. exudated organic acids, which could significantly mobilize sparingly soluble phosphate. There were obvious differences among various genotypes, showing differences between Astragalus L. varieties. Cultivating Astragalus L. could be used to improve soil P-deficiency nutrient cycling in red soils region of southern China, but further research should be carried out to scientifically evaluate organic acids on the activation capacity of Al-P and Fe-P.

Key words: Astragalus L., root exudate, sparingly soluble phosphorus, mobilization, organic acid, P-deficiency stress

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