大豆LEA基因家族全基因组鉴定、分类和表达

doi:10.3864/j.issn.0578-1752.2011.19.004

Abstract

Abstract: 【Objective】 Identification, phylogenetic and tissue specific expression pattern analysis of LEA family genes in Soybean will be helpful to study the functions of plant LEA genes. 【Method】 Based on soybean genome database and bioinformatic method, soybean LEA family genes were identified and their gene sequences, chromosome location and duplication information were obtained. LEA proteins were classified according to their structure and phylogeny relationship. The soybean developmental expression database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages.【Result】A total of 36 LEA genes were systematically identified from soybean and classified into 8 subfamilies according to motif analysis and phylogeny relationship. They were located on 16 chromosomes and most of them had stress related cis-acting elements in their promoter regions. Ten differentially expressed genes were found, and five of them were highly expressed in seeds by microarray and EST data at each developmental stages.【Conclusion】Thirty six LEA family genes in soybean were identified by genome-wide screening. They were classified into 8 subfamilies and distributed on 16 chromosomes with stress related cis-acting elements in their promoter regions. They were differentially expressed in different tissues and developmental stages. These results are helpful for their functional analysis and utilization in crop genetic improvement.

Key words:

LEA gene family, genome-wide identification, phylogeny analysis, differentially expressed gene

LI Le, XU Hong-Liang, YANG Xing-Lu, LI Ya-Xuan, HU Ying-Kao. Genome-Wide Identification, Classification and Expression Analysis of LEA Gene Family in Soybean[J].Scientia Agricultura Sinica, 2011, 44(19): 3945-3954.

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Genome-Wide Identification, Classification and Expression Analysis of LEA Gene Family in Soybean

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