Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3529-3536.doi: 10.3864/j.issn.0578-1752.2011.17.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of Nitrogen and PP333 Application on the Lignin Synthesis of Stem in Relation to Lodging Resistance of Wheat

CHEN  Xiao-Guang, SHI  Yu-Hua, WANG  Cheng-Yu, YIN  Yan-Ping, NING  Tang-Yuan, SHI  Chun-Yu, LI  Yong, WANG  Zhen-Lin   

  1. 1. 山东农业大学农学院/作物生物学国家重点实验室
    2. 江苏徐州甘薯研究中心
  • Received:2010-12-09 Revised:2011-01-24 Online:2011-09-01 Published:2011-01-25

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Abstract: 【Objective】 This study was carried out to elucidate the effects of nitrogen and PP333 application on lignin content in culm of wheat, and discuss the physiological mechanism of nitrogen and PP333 in regulating the lodging resistance of wheat. 【Method】A field experiment was conducted with two nitrogen application rates and two PP333 levels to study the changes of snapping resistance, culm lodging resistance index (CLRI), lignin content and activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), cinnamyl alcohol dehydrogenase (CAD) and 4-coumarate CoA ligase (4CL) of wheat, and the effect of nitrogen and spraying PP333 on the lignin content, related enzyme activities in the lignin biosynthesis of wheat and their relationships with lodging resistance.【Result】 Compared with the low N (225 kg•hm-2) rate, the high N (300 kg•hm-2) rate reduced the PAL, TAL, CAD and 4CL activities and lignin content, reduced snapping resistance and CLRI of the same cultivar. The application of PP333 improved the PAL, TAL and CAD activities, increased lignin content, snapping resistance and CLRI, thus reduced lodging area and lodging degree. Correlation analysis showed that the lignin content of basal stem had a significant and positive correlation with PAL, CAL and CAD activity and CLRI, respectively, yet had no significant correlation with 4CL activity.【Conclusion】The high N(300 kg•hm-2) rate reduced the PAL, TAL,CAD enzyme activities and lodging resistance. Spraying PP333 improved the PAL, TAL, CAD enzyme activities and lignin content, and thus enhanced the lodging resistance.

Key words: Wheat, Lignin, Enzyme activity, Culm lodging resisitant index(CLRI)

CLC Number: 

  • S512.1+1

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