Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (11): 2387-2397 .doi: 10.3864/j.issn.0578-1752.2011.11.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Development of an Immunochromatographic Strip Test for Detection of Neomycin

WANG Ai-ping, LI Fa-di, HU Xiao-fei, SHI Zhao-guo, ZHOU Jing-ming, DU Xiao-ming, WANG Lin-lin, YU Le-le, LIU Ming-yang   

  1. 甘肃农业大学动物科学技术学院
  • Received:2011-04-08 Revised:2011-05-09 Online:2011-06-01 Published:2011-06-01
  • Contact: ai ping wang

Abstract: 【目的】研制新霉素(neomycin,NEO)免疫膜层析快速检测试纸。【方法】建立分泌抗新霉素单克隆抗体(NEO mAb)杂交瘤细胞株,用胶体金免疫膜层析试验(GICA)制备NEO残留快速检测试纸(NEO-Strip),并对其性能进行鉴定。【结果】抗新霉素单克隆抗体的亲和力常数为3.75×1010 mol·L-1;制备的 NEO-Strip目测限为50 ng·mL-1;试纸可特异性检测NEO,与庆大霉素、链霉素、妥布霉素、紫苏霉素、莱克多巴胺、沙丁胺醇、泰乐菌素和氯霉素等其它药物均无交叉反应性;就定性检测而言,其结果与高效液相色谱串联质谱法(HPLC-MS-MS)完全一致;不同的基质液对试纸的敏感度影响甚微。【结论】建立了特异的NEO免疫层析检测方法。

Key words: neomycin, monoclonal antibody, colloidal gold particles, test strip

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