新霉素免疫膜层析检测方法研究

doi:10.3864/j.issn.0578-1752.2011.11.023

Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (11): 2387-2397 .doi: 10.3864/j.issn.0578-1752.2011.11.023

• VETERINARY SCIENCE • Previous Articles Next Articles

Development of an Immunochromatographic Strip Test for Detection of Neomycin

^{WANG Ai-ping, LI Fa-di, HU Xiao-fei, SHI Zhao-guo, ZHOU Jing-ming, DU Xiao-ming, WANG Lin-lin, YU Le-le, LIU Ming-yang}

甘肃农业大学动物科学技术学院

Received:2011-04-08 Revised:2011-05-09 Online:2011-06-01 Published:2011-06-01
Contact: ai ping wang

Abstract

Abstract: 【目的】研制新霉素（neomycin，NEO）免疫膜层析快速检测试纸。【方法】建立分泌抗新霉素单克隆抗体(NEO mAb)杂交瘤细胞株，用胶体金免疫膜层析试验(GICA)制备NEO残留快速检测试纸(NEO-Strip)，并对其性能进行鉴定。【结果】抗新霉素单克隆抗体的亲和力常数为3.75×1010 mol·L-1；制备的 NEO-Strip目测限为50 ng·mL-1；试纸可特异性检测NEO，与庆大霉素、链霉素、妥布霉素、紫苏霉素、莱克多巴胺、沙丁胺醇、泰乐菌素和氯霉素等其它药物均无交叉反应性；就定性检测而言，其结果与高效液相色谱串联质谱法（HPLC-MS-MS）完全一致；不同的基质液对试纸的敏感度影响甚微。【结论】建立了特异的NEO免疫层析检测方法。

Key words: neomycin, monoclonal antibody, colloidal gold particles, test strip

^{WANG Ai-ping,LI Fa-di,HU Xiao-fei,SHI Zhao-guo,ZHOU Jing-ming,DU Xiao-ming,WANG Lin-lin,YU Le-le,LIU Ming-yang}. Development of an Immunochromatographic Strip Test for Detection of Neomycin[J].Scientia Agricultura Sinica, 2011, 44(11): 2387-2397 .

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