Abstract:

【Objective】 To study the character on coagulase genotype distribution of Staphylococcus aureus isolated from bovine mastitis in Shanghai and Guizhou areas in China,provide credible theory evidence for prevention and treatment on bovine mastitis. 【Method】 S.aureus strains were identified by PCR amplification on which primer was designed according to the 16S rRNA conserved sequence. The S.aureus strains have been identified were typed by polymorphism of coagulase gene and it’s fragment disposed by incision enzyme. 【Result】 Seventy-eight S.aureus strains were identified and coagulase gene were amplifid out from 74 S.aureus strains. The strains were separated into five genotypes and two subtypes. PCR1 was preponderant genotype of Guizhou area and PCR3 was preponderant genotype of Shanghai area.【Conclusion】The effects on epidemic of the pathogenic bacteria influenced by diversity of geographical conditions and the levels of breeding may be the reasons of distinct diversity in distribution of different genotypic strains in these two areas.

Key words: bovine mastitis, Staphylococcus aureus, molecular identification, coagulase genotype