固相微萃取,草莓,气-质联用,香气成分,自动质谱解卷积鉴定系统 ," /> 固相微萃取,草莓,气-质联用,香气成分,自动质谱解卷积鉴定系统 ,"/> solid phase micro-extraction ( SPME),strawberry,GC/MS,aroma compounds,AMDIS
,"/> <font face="Verdana">Study of Four Coating Materials of SPME Fiber on Extraction of Aroma Compounds in Strawberry#br# </font>

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (21): 4473-4481 .doi: 10.3864/j.issn.0578-1752.2010.21.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Study of Four Coating Materials of SPME Fiber on Extraction of Aroma Compounds in Strawberry#br#

FU Lei, LIU Zheng-sheng, SUN Xin-yang, ZHU Shu-hua#br#   

  1. (山东农业大学化学与材料科学学院)
  • Received:2010-06-26 Revised:2010-08-12 Online:2010-11-01 Published:2010-11-01
  • Contact: ZHU Shu-hua

Abstract:

【Objective】 In this paper the extraction of strawberry aroma compounds with four coating materials of solid-phase micro-extraction (SPME) fiber and the automatic identification method for gas chromatography/mass spectrometry (GC/MS) data were researched. The aim of this experiment was to summarize a simple, accurate method for quality breeding and processing of strawberry. 【Method】 The aroma compounds in strawberry were detected by SPME-GC/MS- the automatic mass spectral deconvolution and identification system (AMDIS). 【Result】 A total of 97 compounds were identified from the sample extracts. The major constituents were esters, alcohols, ketones and terpenes, with the proportions of 42.3%, 13.4%, 10.3% and 6.2%, respectively. There are at least eight kinds of characteristic aroma components in strawberry. The most important aroma compounds were ethyl butanoate, ethyl hexanoate, linalool, furaneol, methyl butanoate, methyl hexanoate, 2-heptanone and nerolidol according to their aroma values. The 50/30 μm DVB/CAR/ PDMS fiber showed high sensitivity and selectivity for the determination of aroma compounds. The rest sequence was 100 μm PDMS,85 μm PA and 7 μm PDMS fibers in turn. Four SPME fibers had the similar extraction effect to ester. 50/30 μm DVB/CAR/PDMS fiber had significantly higher extraction effect than other fibers to ketones, terpenes, alcohols and aldehydes. 【Conclusion】 50/30 μm DVB /CAR /PDMS fiber is suitable for the extraction of aroma compounds of strawberry. The AMDIS is sensitive, accurate and rapid. It is worth to be popularized and applied.

Key words: solid phase micro-extraction ( SPME)')">solid phase micro-extraction ( SPME), strawberry, GC/MS, aroma compounds, AMDIS

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