Abstract:

【Objective】 Developing an ELISA based on a synthetic peptide detecting foot-and-mouth disease virus(FMDV) nonstructural proteins (NSPs) antibody. 【Method】 A specific peptide according to FMDV NSPs B-cell epitopes was synthesized by a solid-phase method, and was conjugated with carrier protein BSA. An ELISA for detecting FMDV NSPs antibody was developed by using the conjugating protein as the coating antigen. 【Result】 The optimal coating concentration of the antigen was determined as 2.5μg•mL-1; The results of comparison of the assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in detection of 199 serum samples showed that they were very coincident, and the agreement rate of them reached 96.48% and 97.48, respectively. 【Conclusion】 The developed ELISA using the synthetic peptide as coating antigen is specific, reproducible, stable and easy, and can be used to differentiate FMDV infected animals from immunized animals.

Key words: FMDV')">FMDV, ELISA, Synthetic peptide