Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (06): 1282-1286 .doi: 10.3864/j.issn.0578-1752.at-2005-5148

• RESEARCH NOTES • Previous Articles     Next Articles

Sequence Comparison on Gene orf224 of Polima CMS and Shan2A CMS in Brassica napus L.

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  • Received:2005-02-02 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10

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Abstract: 【Objective】The 4.5kb section including gene orf224 in Mitochondrial genomes of Polima CMS in Brassica napus L.is correlated with cytoplasmic male-sterility. 【Method】 To prove the difference of Shan2A CMS and Polima CMS in gene orf224, two special primers of 5′ and 3′ were designed according to gene orf224 in this research. Mitochondrial genomes of Polima CMS and Shan2A CMS were amplified with two primers above. The homologous DNA section of orf224 was gained in Shan2A CMS and sequenced. 【Result】The results showed that the two sequences both comprise 675 bases. The homology of the two sequences is 99.3%. 【Conclusion】Thus it has proved that Shan2A CMS is different from Polima CMS in gene orf224. So we can't take Shan2A CMS as Polima CMS simply from the relation of maintainer and restorer.

Key words: Brassica napus L, Polima CMS, Shan2A CMS, orf224, Sequencing

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