Abstract: 【Objective】The aim of the experiment is to systematically investigate the effects of pollination combination, development stage of male flower when subjected to irradiation and the dosage on fruit setting and haploid production of cucumber. 【Method】The methods of irradiated once and pollinated twice plus embryo culture were adopted, 32 different genotypes of cucumber were used as maternal parents and 6 genotypes were used as paternal parents to produce haploid cucumber through γ-irradiated pollen pollination. 【Result】 Totally 48 haploid embryos were successfully recovered from 15 genotypes, and the average percentage of haploid embryo production was 0.77% and the highest was 50.00%.【Conclusion】 No significant influence of male flowers developing stage on fruit setting was found but the haploid embryo production was affected. The irradiation dosage affected fruit setting and haploid embryo production, but there was no significance. The haploid embryo production was affected greatly by different pollination combinations. The experiments from morphological observation, meiotic chromosome behavior and fertility investigation, and some physiological and biochemical measurements indicated that there were significant morphological differences between haploid and diploid. Most haploid plants had no fertility except H2. Meiotic observation on the H2 found the abnormal behavior at every stage, but there was a production of 0+7 gametes produced at very low frequency at anaphase I, which likely produced the fertile gametes. Differences in chlorophyll contents, activation of POD, soluble protein contents were found between haploid and diploid plants.

Key words: Cucumber (Cucumis sativus L.), irradiated pollen, embryo rescue, meiosis, Meiosis, Haploid