Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (14): 3185-3202.doi: 10.3864/j.issn.0578-1752.2026.14.014

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Nutritional and Processing Characteristic Evaluation and Proteomics Analysis of Chicken Cultured Meat

DUAN RuiPei(), XIA ShuangMei, ZHANG Yi, GUO YuJie, ZHANG Xing(), ZHANG ChunHui   

  1. Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Comprehensive Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193
  • Received:2025-12-19 Accepted:2026-04-27 Online:2026-07-16 Published:2026-07-21
  • Contact: ZHANG Xing

Abstract:

【Objective】This study aimed to clarify the differences in nutritional and processing qualities between cultured meat (CM) and chicken breast (CB), chicken thigh (CT), and to use proteomic technology to elucidate the quality differences, thereby providing a theoretical basis for the food processing of cultured meat.【Method】The compositional differences among CM, CB and CT in proteins, water, fat, vitamins, minerals, fatty acids, and amino acids were evaluated to assess the nutritional quality of CM. pH values, color differences, and water holding capacity were measured to evaluate the processing quality of CM. Finally, proteomic analysis was used to identify differentially expressed proteins among CM, CB and CT, with metabolic pathways annotated using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) to explain the compositional differences.【Result】CM had higher water content and lower protein content compared with CB and CT, but significant differences were observed in fatty acids, vitamins, and amino acids. The predominant fatty acid in cultured meat was oleic acid (C18:1), while the saturated fatty acid (SFA) content in CM was lower than that in CB and CT. Glutamic acid constitutes the primary amino acid (99.55 mg/100 g), cysteine and tyrosine levels were higher. However, the proportion of essential amino acids (34.99%) was lower than that in CB and CT. Vitamin B1 content (0.09 mg/100 g) in CM was intermediate between CB and CT, but vitamin E content (0.01 mg/100 g) was lower than that in CB and CT. Potassium was also lower than that in CB and CT, while aluminum, iron, and selenium content exceeded those in CB and CT. The hardness and chewiness of CM were lower than those in CB and CT, the loss of centrifugal force was higher than that of CB (14.57%) and CT (12.56%), and the cooking loss was almost the same as that of CB, but significantly lower than that of CT (P<0.05). Through proteomic analysis with VIP>1, 76 differentially expressed proteins were identified among CB, CT and CM, primarily including fructose-1, 5-bisphosphate aldolase (ALDOC) and myosin light chain (MYL1). Structural proteins such as myofibrillar proteins in CM, along with proteins related to muscular system processes and skeletal muscle development, were downregulated. Conversely, fibroblast-associated proteins exhibited up-regulation, such as KANK1, TPM2, and COL5A1.【Conclusion】CM was different from raised meat in nutritional quality. It contained lower protein and essential amino acid ratios while having higher sodium levels than raised meat. However, it demonstrated relatively lower SFA content and contained unsaturated fatty acids. CM also contained higher levels of microelements like iron and selenium, indicating potential for nutritional fortification. The texture of CM was softer, with lower water retention capacity than raised meat, making it more suitable for processing into emulsified sausages and other minced meat products. Proteomic analysis revealed down-regulated expression of structural proteins, such as myofibrillar proteins in CM, resulting in weaker thermal gelation ability and water holding capacity, which might be key factors contributing to its texture differences.

Key words: chicken, cultured meat, nutritional quality, processing quality, quality difference

Table 1

Mobile phase elution program"

时间 Time (min) B (%)
0 8
1 17
5.5 45
7 99
8 99

Fig. 1

Basic nutritional composition comparison of chicken breast, chicken thigh, and cultured meat CB: Chicken breast; CT: Chicken thigh; CM: Cultured meat. Different lowercase letters indicate significant differences (P<0.05). The same as below"

Fig. 2

Fatty acid composition of chicken breast, chicken thigh, and cultured meat"

Table 2

Fatty acid content of chicken breast, chicken thigh, and cultured meat"

类型
Type
含量 Content (mg/100 g)
CB CT CM
饱和脂肪酸Saturated fatty acids (SFA) 1359.85±24.10a 1239.24±3.42b 953.14±6.79c
单不饱和脂肪酸Monounsaturated fatty acids (MUFA) 1745.26±11.9a 1726.42±10.58a 841.83±4.83b
多不饱和脂肪酸Polyunsaturated fatty acids (PUFA) 1487.78±5.40a 1338.82±11.32b 121.52±2.82c

Fig. 3

Amino acid composition of chicken breast, chicken thigh, and cultured meat"

Table 3

Vitamin composition of chicken breast, chicken thigh, and cultured meat"

类型
Type
含量 Content (mg/100 g)
CB CT CM
维生素B1 Vitamin B1 0.04±0.00c 0.15±0.00a 0.09±0.00b
维生素E Vitamin E 0.60±0.01a 0.38±0.00b 0.01±0.00c

Fig. 4

Macroelement (A) and microelement (B) of chicken breast, chicken thigh, and cultured meat"

Table 4

pH value and color of chicken breast, chicken thigh, and cultured meat"

CB CT CM
pH 5.76±0.02c 6.04±0.08b 6.63±0.08a
L* 50.34±0.26a 47.49±0.20b 45.06±0.38c
a* 2.19 ±0.08c 9.05 ±1.06b 13.91±0.91a
b* 13.82±0.25b 10.14±0.28c 38.98±0.42a

Fig. 5

Water-holding capacity of chicken breast, chicken thigh, and cultured meat"

Table 5

Texture of chicken breast, chicken thigh, and cultured meat"

CB CT CM
硬度Hardness (N) 85.78±6.88a 43.00±1.83b 1.44±0.18c
内聚性Cohesiveness (%) 0.61±0.08a 0.57±0.08a 0.37±0.12b
弹性Springiness (mm) 5.32±0.28a 5.61±0.79a 0.92±0.23b
胶粘性Gumminess (N) 42.48±3.22a 24.15±1.81b 0.52±0.12c
咀嚼性Chewiness (mJ) 281.72±10.63a 131.59±26.88b 0.46±0.02c

Fig. 6

SDS-PAGE electrophoresis profile of chicken breast, chicken thigh, and cultured meat"

Fig. 7

Distribution and number of peptides (A), Coverage of proteins by the identified peptides (B), PCA score plot of among CB, CT, and CM groups (C)"

Table 6

DAPs between CM/CB and CM/CT groups"

CM/CB CM/CT
总差异表达蛋白
Differential expressed protein (DAPs)
3779 3916
上调 Up-regulated 2400 2483
下调 Down-regulated 1379 1433

Fig. 8

Venn of differentially abundant proteins in the different comparison groups (A), Volcano plots of the CM versus CB comparison (B), CM versus CT comparison (C)"

Fig. 9

Hierarchical cluster analysis of differentially abundant proteins"

Fig. 10

GO classification (A) and functional enrichment analysis of DAPs (B)"

Fig. 11

(A) KEGG classification and (B) functional enrichment analysis of DAPs"

Table 7

Structure-related differentially abundant proteins"

GO术语Go terminology Accession Symbol 蛋白质名称 Protein name FC (CM/CB) FC (CM/CT)
肌肉系统过程
Muscular system process
Q05705 BRT-2 原肌球蛋白β链 Tropomyosin beta chain 5.1104 3.0844
P19352 TPM2 原肌球蛋白β链 Tropomyosin beta chain 0.0287 0.0060
A0A8V0Y7H5 TPM2 原肌球蛋白β链 Tropomyosin beta chain 6.6361 3.7284
Q8AWI4 TPM1 原肌球蛋白α链 Tropomyosin alpha chain 0.0238 0.0439
P02588 TNNC2 肌钙蛋白C Troponin C 0.0012 0.0013
A0A8V1A2M9 TNNI2 肌钙蛋白I Troponin I 0.0017 0.0020
P12620 TNNT3 肌钙蛋白T Troponin T 0.0061 0.0018
A0A8V1A1P8 TNNT3 肌钙蛋白T3 Troponin T3 0.0049 0.0053
A0A8V0ZYD0 TPM1 原肌球蛋白1 Tropomyosin 1 0.0526 0.0900
P02565 MYH1B 肌球蛋白1B Myosin 1B 0.0152 0.0094
Q8AY28 N127 肌球蛋白重链HCIII Myosin heavy chain HCIII 0.0090 0.0013
Q9DGM5 肌球蛋白重链亚型2 Myosin heavy chain isoform 2 0.0026 0.0071
A0A8V0WZV4 MYLPF 肌球蛋白轻链 Myosin light chain 0.0018 0.0021
A0A8V0YH72 MYLK2 肌球蛋白轻链激酶2 Myosin light chain kinase 2 0.0747 0.0680
A2NBE2 肌球蛋白轻链激酶2 Myosin light chain kinase 2 0.0033 0.0049
Q91006 原肌球调节蛋白 Tropomodulin 0.0736 0.0291
A0A8V1AHA1 TMOD4 原肌球调节蛋白4 Tropomodulin 4 0.0051 0.0067
P24797 ATP1A2 钠/钾转运ATP酶亚基α-2
Sodium/potassium-transporting ATPase subunit alpha-2
0.0781 0.0522
骨骼肌组织发育
Skeletal muscle tissue development
A0A8V0WZV4 MYLPF 肌球蛋白轻链 Myosin light chain 0.0018 0.0021
A0A088T337 MSTN 生长/分化因子8 Growth/differentiation factor 8 0.0017 0.0029
纤维组织
Fibril organization
A0A8V0Y4B8 KANK1 1401.1770 1401.1770
A0A8V1A0T5 VILL 1204.3600 1204.3600
A0A8V1ADR4 COL5A1 胶原蛋白V型α1链 Collagen type V alpha 1 chain 761.8900 761.8900
A0A8V1A093 含GAR结构域蛋白 GAR domain-containing protein 260.9450 260.9450
Q5ZKU6 CNN2 钙调理蛋白 Calponin 5.8619 10.1907
D5LPR1 FSCN1 肌动蛋白结合蛋白1 Actin-binding protein 1 9.4542 10.1858
A0A8V1AB53 MYO19 非常规肌球蛋白-XIX Unconventional myosin-XIX 3.9571 4.8010
P18359 DSTN 肌动蛋白解聚因子 Actin-depolymerizing factor 4.3293 4.7479
A0A1D5PM19 MYH9 肌球蛋白9 Myosin 9 4.2076 3.6968
A0A8V0ZKF9 MYL9 肌球蛋白轻链9 Myosin light chain 9 3.7640 3.3864
Q8AWI4 TPM1 原肌球蛋白2 Tropomyosin 2 0.0238 0.0439
Q9DEH4 伴肌动蛋白 Nebulin 0.0004 0.0019
A0A8V1AA47 MYO1G 肌球蛋白IG Myosin IG 0.0011 0.0007
A0A8V0WZP8 MYBPC2 肌球蛋白结合蛋白C Myosin-binding protein C 0.0028 0.0005
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