Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (11): 2106-2115.doi: 10.3864/j.issn.0578-1752.2018.11.008

• PLANT PROTECTION • Previous Articles     Next Articles

Differential Expressed Genes and their Pathways of the  Resistance to Flubendiamide in Plutella xylostella

WANG ChengHua, SUN ShiQing, XU JuLong, ZHAO XiaoLong, XUE ChaoBin   

  1. College of Plant Protection, Shandong Agricultural University/Provincial Key Laboratory of Pesticide Toxicology and Application Technique, Taian 271018, Shandong
  • Received:2017-12-27 Online:2018-06-01 Published:2018-06-01

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Abstract: 【Objective】With the widespread use of diamide insecticides, such as flubendiamide, the resistance of Plutella xylostella to these insecticides became more and more prominent. The objective of this study is to research the resistant mechanism to flubendiamide in P. xylostella from the transcriptome perspective, identify the key genes and pathways that lead to resistance to flubendiamide in P. xylostella, and to lay a foundation for revealing the mechanism of resistance to flubendiamide in P. xylostella.【Method】 The resistance to flubendiamide in P. xylostella was determined by the RNA sequencing technique (RNA-Seq) using the flubendiamide-resistant strains (Rh28), the field resistant population (Rz36) and the susceptible strain (S). The differential expressed genes (DEGs) and significantly up-regulated resistant genes were obtained in the strains/populations. The enrichment analysis of Gene Ontology (GO) and the enrichment analysis of Kyoto Encyclopedia of Gene and Genome (KEGG) were used to identify DEGs. The major biological functions, the major biochemical metabolic and signaling pathways of the DEGs were elaborated. 【Result】The different numbers of DEGs were obtained by comparing RNA-Seq data of the different resistant strains/populations with susceptible strains. GO analysis of the biological process (BP) and pathway for differential gene enrichment showed that they were mainly focused on response to stimulus, catalytic activity and metabolic process items. KEGG analysis found that the differential genes were mainly concentrated in the metabolic pathway, with the highest number of genes (17.84%). the genes with significantly up-regulated expression were further obtained, and GO analysis was carried out on 218 of them, which still mainly focused on the metabolic process, response to stimulus and biological regulation items. KEGG analysis of significantly up-regulated expression genes found that the DEGs were still mainly in the metabolic pathway. The genes of glutathione-S-transferase, cytochrome P450 detoxification enzymes, and involved in a variety of insect physiological responses to heat shock protein (Hsp) family genes were significantly up-regulated compared with the susceptible strain. The results also showed that most of the genes with significant up-regulation were concentrated in protein processing in endoplasmic reticulum, tyrosine metabolism, caffeine metabolism and the Wnt signaling pathway by heat map and clustering analysis.【Conclusion】the DEGs and the significantly up-regulated expression genes were obtained in resistant strain of P. xylostella, which of them were mainly involved in the metabolic process, the response to stress and the response to stimulus. The synergistic regulation of these genes is an important mechanism of resistance to flubendiamide in P. xylostella.

Key words: RNA sequencing, differential expressed genes, flubendiamide, resistance, Plutella xylostella

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