Abstract: 【Objective】The aim of this study is to clone and analyze soybean protein gene GmSABP2, which is binded with salicylic acid, and transform Arabidopsis for analyzing salt tolerance and drought tolerance, and further understand the molecular mechanism of salt tolerance and drought-resistance of the gene. 【Method】 Using SABP2 as a probe, the soybean genome database was searched, and the highest sequence homology was picked out and named as GmSABP2. The gene GmSABP2 was cloned by using electronic cloning technology. The DNAMAN program was used to analyze the amino acid sequence alignment and the conserved domain amino acid by the CD-search conducted NCBI. The MEGA program was applied to make the phylogenetic analysis. The phenotypic variation of soybean seedlings under salt and drought stress was analyzed. The expression of the characteristics of GmSABP2 under salt and drought conditions was analyzed by Real time-PCR of soybean seedlings. Gateway technology was used to build plant expression vector pEarleyGate103-GmSABP2, shifted into Agrobacterium tumefaciens EHA105, infected Arabidopsis by utilizing flower dip method, then the homozygous transgenic plants were obtained by resistance screening and finally the salt and drought tolerance was analyzed. The wild-type plants and transgenic plants were treated under salt and drought stresses, and both the seed germination, root length and mature plants were counted under stress conditions. 【Result】The cDNA sequence of GmSABP2 was obtained and the open reading frame is 786 bp and total length of the sequence is 1 235 bp, encoding 261 amino acids. And molecular weight is 29.15 kD, an isoelectric point is 5.58. The amino acid sequence alignment and phylogenetic analysis showed that GmSABP2 and tobacco SABP2, Rauvolfia serpentina PNAE had the highest similarity. Using the CD-search of NCBI, it was found that the Abhydrolase_6 (pfam: 12697) as conserved domain hydrolases. Soybean SABP2 protein belongs to SABP2 α/β hydrolase superfamily. Using MEGA program to build a system of species multiple phylogenetic tree, it was found that SABP2 of soybeans, Theobroma cacao SABP2 and Solanum lycopersicum SABP2 have a close genetic relationship, but has a distant genetic relationship with Arabidopsis SABP2. The phenotype of soybean seedlings under salt and drought conditions was analyzed and it was found that there were significant stress effects. Real time-PCR analysis showed that GmSABP2 under salt and drought conditions were upregulated expression. Arabidopsis thaliana resistance analysis showed that under normal culture conditions, the wild-type plants and transgenic plants could germinate and grow. Under 150 mmol·L^-1 NaCl treatment conditions, seed germination rate of wild-type plants was 38%, seedling root length after 12 days was 0.4 cm and the survival rate of mature plants was 49%; The seed germination rate of transgenic lines was 67%, seedling root length after 12 days was 1.1 cm and mature plants survival was 78%. Under 20% PEG6000 treatment conditions, the seed germination rate of wild-type plants was 31%, seedling root length after 12 days was 0.5 cm and mature plants survival rate was 36%; The seed germination rate of transgenic lines was 57%, seedling root length after 12 days was 1.0 cm and mature plants survival rate was 66%.【Conclusion】 The GmSABP2 gene increases the resistance of Arabidopsis plants under salt and drought conditions.

Key words: soybean, GmSABP2, tissue expression analysis, transgenic Arabidopsis, functional analysis