Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1646-1656.doi: 10.3864/j.issn.0578-1752.2013.08.014

• HORTICULTURE • Previous Articles     Next Articles

Effects of NaCl Stress on Seed Germination and DNA Methylation Status Detected by MSAP Analysis in Cucumber

 HUANG  Yun-Yu, ZHANG  Hai-Jun, XING  Yan-Xia, QI  Yan, SUN  Qian-Qian, ZHOU  Chun-Lei, ZHAO  Bing, GUO  Yang-Dong   

  1. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193
  • Received:2012-12-11 Online:2013-04-15 Published:2013-03-04

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Abstract: 【Objective】The objectives of this study were to analyze the cytosine methylation dynamics during cucumber seed germination and to assess the effect of NaCl treatment on seed germination and DNA methylation status. Moreover, the role of DNA methylation on seed germination and salt stress adaptation was studied at the same time.【Method】Methylation sensitive amplified polymorphism (MSAP) method was used to analyze the level and variation patterns of DNA methylation at different times (0, 1, 2, 4, 6, 8 d) during cucumber seed germination in different NaCl treatments (0, 150, 200 mmol•L-1).【Result】MSAP results showed that the methylation level in cucumber dry seeds was 15.25%, mainly in full methylation pattern. The level of global DNA methylation decreased during the whole germination process, with a slight increase at the early stages (1-2 d). NaCl treatment aggravated these changes, and made the final level of DNA methylation lower. Hypermethylation and hypomethylation happened simultaneously, and predominated at different germination stages. Among different change types, CG/CHG (H=A, T, C) simultaneous changes were the dominating types. DNA methylation changes were detected in both coding region and non-coding region on the cucumber genome.【Conclusion】DNA methylation changes during seed germination is a complex process. Methylation and demethylation happened at the same time in a temporal-spatial specific way. NaCl treatment reduced the cucumber genome stability and inhibited the seed germination.

Key words: cucumber , germination , DNA methylation , NaCl , methylation sensitive amplified polymorphism

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