Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1106-1115.doi: 10.3864/j.issn.0578-1752.2012.06.008

• PLANT PROTECTION • Previous Articles     Next Articles

Construction of SSH-cDNA Libraries and EST Analysis in Roots of Xiaoli Black Bean in Response to Heterodera glycines Parasitization

 WANG  Fang, DUAN  Yu-Xi, CHEN  Li-Jie, WANG  Yuan-Yuan, ZHU  Xiao-Feng, WANG  Dong   

  1. 1.沈阳农业大学植物保护学院，沈阳 110866
    2.黑龙江省农科院齐齐哈尔分院，黑龙江齐齐哈尔 161006
  • Received:2011-06-13 Online:2012-03-15 Published:2011-10-27

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Abstract: 【Objective】The objective of this study is to research into the early stage root gene expression profile of G. max induced by H. glycines and to explore the resistant mechanism to the pathogen at molecular level. 【Method】SSH-cDNA libraries enriched with differentially expressed ESTs were constructed from SCN-challenged root tissues at pre-penetration and early infection stages from Xiaoli black bean (G. max) by suppression subtractive hybridization and reverse Northern blot. 【Result】 One hundred and sixty-six unique ESTs were identified and analyzed with Blastx and Blastn by comparing sequences in the GenBank. One hundred and ninteen unigenes accounted for 83% of all the unigenes showed high homology with the function known genes or proteins. The function-known ESTs were annotated into functional categories including signal recognition and transduction, energy and material metabolism, stress responses, transcriptional regulation, protein synthesis and/or modification, transport functions, cellular architecture.【Conclusion】Discreet gene tag clusters primarily including catalase, ubiquitin, chitinase, lipoxygenase, aquaporin, ripening related protein, metallothionein, plasma membrane intrinsic protein, cytochrome P450, glyceraldehyde-3-phosphate dehydrogenase were abundant in the SCN-infected roots. It is speculated that those genes played an important function in the incompatible interaction between Xiaoli black bean and H. glycines.

Key words: Heterodera glycines, Glycine max, suppression subtractive hybridization, EST

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