Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (21): 4350-4357.doi: 10.3864/j.issn.0578-1752.2011.21.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transformation of Wheat with High Molecular Weight Glutenin Subunit Gene 1Bx14

 LIU  Xiang-Li, JIN  Wei-Bo, LIU  Jin, ZHAO  Hui-Xian, GUO  Ai-Guang   

  1. 1.西北农林科技大学生命科学学院，陕西杨陵712100
    2.陕西省农业分子生物学重点实验室，陕西杨陵712100
  • Received:2011-04-28 Online:2011-11-01 Published:2011-09-21

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Abstract: 【Objective】 The objective of this study is to use genetic transformation of high quality HMW-GS gene 1Bx14 to improve wheat bread-making quality. 【Method】 This investigation, based on the clone of HMW-GS gene 1Bx14 and construction of plant expression vectors, transformed the immature embryo of Mianyang19 by microprojectile bombardment. After selection with hygromycin and tested by PCR, the positive progenies were confirmed by PCR-Southern and Southern blotting. The compositions of high molecular weight glutenin subunit were analyzed by SDS-PAGE.【Result】Six transformed plantlet was obtained from 652 transformed callus, yielding a transformation frequency of 0.92%. The HMW-GS gene 1Bx14 was expressed in some seeds of transgenic plants with the expression of other subunits suppressed in varying degrees. 【Conclusion】The high molecular weight glutenin subunit gene 1Bx14 was transformed into wheat Mianyang 19 and was expressed in some transgenic progenies.

Key words: wheat, high molecular weight glutenin subunit, 1Bx14, transformation

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